Matrix vesicles(MVs)have shown strong effects in diseases such as vascular ectopic calcification and pathological calcified osteoarthritis and in wound repair of the skeletal system due to their membranous vesicle cha...Matrix vesicles(MVs)have shown strong effects in diseases such as vascular ectopic calcification and pathological calcified osteoarthritis and in wound repair of the skeletal system due to their membranous vesicle characteristics and abundant calcium and phosphorus content.However,the role of MVs in the progression of osteoporosis is poorly understood.Here,we report that annexin A5,an important component of the matrix vesicle membrane,plays a vital role in bone matrix homeostasis in the deterioration of osteoporosis.We first identified annexin A5 from adherent MVs but not dissociative MVs of osteoblasts and found that it could be sharply decreased in the bone matrix during the occurrence of osteoporosis based on ovariectomized mice.We then confirmed its potential in mediating the mineralization of the precursor osteoblast lineage via its initial binding with collagen type I to achieve MV adhesion and the subsequent activation of cellular autophagy.Finally,we proved its protective role in resisting bone loss by applying it to osteoporotic mice.Taken together,these data revealed the importance of annexin A5,originating from adherent MVs of osteoblasts,in bone matrix remodeling of osteoporosis and provided a new strategy for the treatment and intervention of bone loss.展开更多
目的通过比较危重症患者膀胱温与直肠温、耳温的差异性及相关性,探讨膀胱温应用于危重症患者体温监测的可行性。方法2018年1月至2019年5月,便利抽样法选取广州市某三级甲等医院重症监护室(intensive care unit,ICU)收治的47例重症患者...目的通过比较危重症患者膀胱温与直肠温、耳温的差异性及相关性,探讨膀胱温应用于危重症患者体温监测的可行性。方法2018年1月至2019年5月,便利抽样法选取广州市某三级甲等医院重症监护室(intensive care unit,ICU)收治的47例重症患者为研究对象,分别测量其入科后24h内6个时间点(2:00、6:00、10:00、14:00、18:00、22:00)的膀胱温与直肠温、耳温。采用重复测量方差分析及组内相关系数,比较三种体温测量结果间的差异性及相关性。结果膀胱温与直肠温、耳温的差异均有统计学意义(均P<0.001),与测量时间无交互作用。直肠温(r=0.91,P<0.05)、耳温(r=0.70,P<0.05)与膀胱温呈正相关。结论膀胱温与直肠温、耳温相关性较好,是ICU重症患者监测体温的可靠方法之一;且膀胱温能够持续监测,有助于减轻护士工作量,降低交叉感染发生率,值得在ICU中推广。展开更多
目的分析近10年衰弱领域文献,了解衰弱领域发展现状及国际研究趋势。方法以2008-2018年期间在Web of Science数据库收录的10 686篇衰弱相关文献为数据来源,分别以关键词、被引文献为分析对象,应用CiteSpace软件进行关键词共现分析和文...目的分析近10年衰弱领域文献,了解衰弱领域发展现状及国际研究趋势。方法以2008-2018年期间在Web of Science数据库收录的10 686篇衰弱相关文献为数据来源,分别以关键词、被引文献为分析对象,应用CiteSpace软件进行关键词共现分析和文献共被引分析。结果近10年衰弱领域研究热点集中于衰弱流行病学调查、不同衰弱状态下老年患者疾病治疗方案的制定、运动训练对老年人身体功能的影响;认知衰弱和衰弱联合干预是衰弱领域现阶段的研究前沿。结论衰弱领域的研究仍处于发展阶段,选择并确定高效的、合适临床环境的衰弱评估工具意义重大,在国内不同地区、不同场所开展衰弱的流行病学调查很有必要,联合衰弱干预措施是否具有更好的效果仍需进一步探究。临床上,应根据衰弱状态为老年患者提供个性化的医疗护理服务。展开更多
目的了解专业型护理硕士研究生职业同一性水平,并分析其影响因素。方法2018年12月至2019年1月,便利抽样法选取全国33所普通高校的185名护理硕士专业学位(master of nursing specialist,MNS)研究生为研究对象,采用一般资料问卷、大学生...目的了解专业型护理硕士研究生职业同一性水平,并分析其影响因素。方法2018年12月至2019年1月,便利抽样法选取全国33所普通高校的185名护理硕士专业学位(master of nursing specialist,MNS)研究生为研究对象,采用一般资料问卷、大学生职业同一性问卷、中文版护理专业学生专业态度量表、大学生生涯社会支持量表及护生职业自我效能问卷对其进行调查。结果专业型护理硕士研究生职业同一性得分的中位数(四分位间距)为94.00(33.00)分。多元线性回归结果显示,性别、第一就业意向、职业自我效能感、专业态度以及生涯社会支持为MNS研究生职业同一性影响因素,可解释度为66.1%。结论专业型护理硕士研究生职业同一性处于中等偏上水平,学校及医院等相关培养机构应重视专业型护理硕士研究生择业问题,制定和实行有针对性的改进措施。展开更多
Tomato leaf mold is a common disease in tomato production and severely impacts the growth,fruit quality and yield of tomato plants.Research on tomato leaf mold has a long history and has focused mainly on the differen...Tomato leaf mold is a common disease in tomato production and severely impacts the growth,fruit quality and yield of tomato plants.Research on tomato leaf mold has a long history and has focused mainly on the differentiation of pathogen races,the structure and function of pathogen Avr gene products,the cloning of Cf resistance genes and the analysis of disease resistance mechanisms.Interactions between Cf and Avr are in accordance with the"gene-for-gene"hypothesis and typical Cf/Avr interactions are part of effector-triggered immunity(ETI).However,an increasing number of studies have proven that pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)is involved in the disease resistance response system mediated by Cf genes.In addition,different Cf genes have both similar and different roles in the disease resistance response,indicating that the disease resistance mechanism of Cf genes is complex.In this paper,progress in tomato leaf mold research was reviewed,and the regulatory mechanism underlying the Cf-mediated disease resistance response was thoroughly explored.We hope this summary will lay a foundation for research on tomato leaf mold disease resistance mechanisms and is applicable to breeding for disease resistance.展开更多
目的评价多维生命意义感量表中文版(the Chinese version of multidimensional existential meaningscale,MEMS-C)在大学生中的适用性。方法将多维生命意义感量表翻译成中文,采用便利抽样法利用问卷星平台抽取453名大学生进行问卷调查,...目的评价多维生命意义感量表中文版(the Chinese version of multidimensional existential meaningscale,MEMS-C)在大学生中的适用性。方法将多维生命意义感量表翻译成中文,采用便利抽样法利用问卷星平台抽取453名大学生进行问卷调查,分析量表的信度和效度。采用多维生命意义感量表对18名大学生进行间隔两周的前后两次调查,以分析量表的重测信度。结果量表验证性因子分析的拟合指标为χ2=450.510,df=74,P<0.01,χ2/df=6.088,GFI=0.859,CFI=0.930,RMSEA=0.106,RMR=0.074,SRMR=0.0494。量表的效标关联效度系数为0.617(P<0.01)。总量表及人生理解、生命目的、生命重要性维度的内部一致性信度系数(Cronbachα)分别为0.935、0.893、0.936、0.733。量表的重测信度为0.908(P<0.01)。结论多维生命意义感量表中文版(MEMS-C)具有良好的信、效度,适用于检验大学生的生命意义感。展开更多
基金supported by the National Natural Science Foundation of China (Grant numbers 11932014 and 31971239)the Sichuan Science and Technology Program (Grant numbers 2022NSFSC0765 and 2022ZYD0079)。
文摘Matrix vesicles(MVs)have shown strong effects in diseases such as vascular ectopic calcification and pathological calcified osteoarthritis and in wound repair of the skeletal system due to their membranous vesicle characteristics and abundant calcium and phosphorus content.However,the role of MVs in the progression of osteoporosis is poorly understood.Here,we report that annexin A5,an important component of the matrix vesicle membrane,plays a vital role in bone matrix homeostasis in the deterioration of osteoporosis.We first identified annexin A5 from adherent MVs but not dissociative MVs of osteoblasts and found that it could be sharply decreased in the bone matrix during the occurrence of osteoporosis based on ovariectomized mice.We then confirmed its potential in mediating the mineralization of the precursor osteoblast lineage via its initial binding with collagen type I to achieve MV adhesion and the subsequent activation of cellular autophagy.Finally,we proved its protective role in resisting bone loss by applying it to osteoporotic mice.Taken together,these data revealed the importance of annexin A5,originating from adherent MVs of osteoblasts,in bone matrix remodeling of osteoporosis and provided a new strategy for the treatment and intervention of bone loss.
文摘目的通过比较危重症患者膀胱温与直肠温、耳温的差异性及相关性,探讨膀胱温应用于危重症患者体温监测的可行性。方法2018年1月至2019年5月,便利抽样法选取广州市某三级甲等医院重症监护室(intensive care unit,ICU)收治的47例重症患者为研究对象,分别测量其入科后24h内6个时间点(2:00、6:00、10:00、14:00、18:00、22:00)的膀胱温与直肠温、耳温。采用重复测量方差分析及组内相关系数,比较三种体温测量结果间的差异性及相关性。结果膀胱温与直肠温、耳温的差异均有统计学意义(均P<0.001),与测量时间无交互作用。直肠温(r=0.91,P<0.05)、耳温(r=0.70,P<0.05)与膀胱温呈正相关。结论膀胱温与直肠温、耳温相关性较好,是ICU重症患者监测体温的可靠方法之一;且膀胱温能够持续监测,有助于减轻护士工作量,降低交叉感染发生率,值得在ICU中推广。
文摘目的分析近10年衰弱领域文献,了解衰弱领域发展现状及国际研究趋势。方法以2008-2018年期间在Web of Science数据库收录的10 686篇衰弱相关文献为数据来源,分别以关键词、被引文献为分析对象,应用CiteSpace软件进行关键词共现分析和文献共被引分析。结果近10年衰弱领域研究热点集中于衰弱流行病学调查、不同衰弱状态下老年患者疾病治疗方案的制定、运动训练对老年人身体功能的影响;认知衰弱和衰弱联合干预是衰弱领域现阶段的研究前沿。结论衰弱领域的研究仍处于发展阶段,选择并确定高效的、合适临床环境的衰弱评估工具意义重大,在国内不同地区、不同场所开展衰弱的流行病学调查很有必要,联合衰弱干预措施是否具有更好的效果仍需进一步探究。临床上,应根据衰弱状态为老年患者提供个性化的医疗护理服务。
文摘目的了解专业型护理硕士研究生职业同一性水平,并分析其影响因素。方法2018年12月至2019年1月,便利抽样法选取全国33所普通高校的185名护理硕士专业学位(master of nursing specialist,MNS)研究生为研究对象,采用一般资料问卷、大学生职业同一性问卷、中文版护理专业学生专业态度量表、大学生生涯社会支持量表及护生职业自我效能问卷对其进行调查。结果专业型护理硕士研究生职业同一性得分的中位数(四分位间距)为94.00(33.00)分。多元线性回归结果显示,性别、第一就业意向、职业自我效能感、专业态度以及生涯社会支持为MNS研究生职业同一性影响因素,可解释度为66.1%。结论专业型护理硕士研究生职业同一性处于中等偏上水平,学校及医院等相关培养机构应重视专业型护理硕士研究生择业问题,制定和实行有针对性的改进措施。
基金supported by grants from the National Natural Science Foundation of China (Grant No.32072589)the Heilongjiang Provincial Natural Science Foundation of China (Grant No.YQ2021C013)。
文摘Tomato leaf mold is a common disease in tomato production and severely impacts the growth,fruit quality and yield of tomato plants.Research on tomato leaf mold has a long history and has focused mainly on the differentiation of pathogen races,the structure and function of pathogen Avr gene products,the cloning of Cf resistance genes and the analysis of disease resistance mechanisms.Interactions between Cf and Avr are in accordance with the"gene-for-gene"hypothesis and typical Cf/Avr interactions are part of effector-triggered immunity(ETI).However,an increasing number of studies have proven that pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)is involved in the disease resistance response system mediated by Cf genes.In addition,different Cf genes have both similar and different roles in the disease resistance response,indicating that the disease resistance mechanism of Cf genes is complex.In this paper,progress in tomato leaf mold research was reviewed,and the regulatory mechanism underlying the Cf-mediated disease resistance response was thoroughly explored.We hope this summary will lay a foundation for research on tomato leaf mold disease resistance mechanisms and is applicable to breeding for disease resistance.
文摘目的评价多维生命意义感量表中文版(the Chinese version of multidimensional existential meaningscale,MEMS-C)在大学生中的适用性。方法将多维生命意义感量表翻译成中文,采用便利抽样法利用问卷星平台抽取453名大学生进行问卷调查,分析量表的信度和效度。采用多维生命意义感量表对18名大学生进行间隔两周的前后两次调查,以分析量表的重测信度。结果量表验证性因子分析的拟合指标为χ2=450.510,df=74,P<0.01,χ2/df=6.088,GFI=0.859,CFI=0.930,RMSEA=0.106,RMR=0.074,SRMR=0.0494。量表的效标关联效度系数为0.617(P<0.01)。总量表及人生理解、生命目的、生命重要性维度的内部一致性信度系数(Cronbachα)分别为0.935、0.893、0.936、0.733。量表的重测信度为0.908(P<0.01)。结论多维生命意义感量表中文版(MEMS-C)具有良好的信、效度,适用于检验大学生的生命意义感。
基金supported by the National Natural Science Foundation of China(No.81600001BJ6)the Jiangsu Province’s Key Provincial Talents Program(No.ZDRCA2016005)+3 种基金the Natural Science Foundation of Jiangsu ProvinceChina(No.BK20141026 DA14)the Jiangsu Science&Technology ProgramChina(No.BE2016002-4)
文摘本研究旨在探讨S100钙结合蛋白A16 (S100 calcium binding protein A16, S100A16)在肝细胞脂质代谢中的作用及可能的生物学机制。用脂肪酸培养HepG2细胞(人肝癌细胞系)以建立脂肪酸培养模型,对照模型不加脂肪酸培养,每一模型分成3组细胞,分别用S100a16过表达质粒、shRNA质粒、Vector质粒转染。用试剂盒检测细胞甘油三酯浓度,用油红O染色观察脂滴聚集情况,用免疫沉淀和质谱分析寻找和S100A16相互作用的兴趣蛋白,并用免疫共沉淀验证,用Western blot和qRTPCR进行相关机制研究。结果显示,和对照模型相比,脂肪酸培养模型细胞内脂肪和甘油三酯浓度显著增加。S100a16过表达组细胞内脂肪积累显著高于Vector质粒转染组。热休克蛋白A5 (heat shock protein A5, HSPA5)与S100A16之间存在相互作用。S100a16过表达可上调内质网应激的HSPA5/肌醇依赖酶1α-X结合蛋白1 (inositol-requiring enzyme 1α-X binding protein 1,IRE1α-XBP1)通路相关蛋白(HSPA5、IRE1α和pIREα1)表达水平,并上调脂肪合成相关基因Srebp1c、Acc和Fas mRNA表达水平,而转染S100A16 shRNA质粒可使上述蛋白和mRNA水平低于Vector质粒转染组。以上结果提示,S100A16可能通过内质网应激HSPA5/IRE1α-XBP1通路促进HepG2细胞脂质合成。
