DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique ...DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique of methylation-sensitive amplified fragment length polymorphism AFLP. Over 19% （42/220） bases were uniformly methy- lated in these tissues. The percentages of polymorphism resulting from varied methylation in mature leaf （L1）, young leaf （L2）, baby leaf （L3）, stem （S）, young root （R1） and lignified root （R2） were 29.5%, 29.0%, 27.1%, 30.7%, 63.0% and 28.3%, respectively. The numbers of poly- morphic loci detected in the leaves of three developmental stages were similar, ranging from 20 to 30. In contrast, roots at the two developmental stages differed greatly, with 145 polymorphic loci detected in R1 and 27 in R2. Our results suggest that the methylation level in leaves slightly increases with aging, while that in roots decreases dramatically with aging.展开更多
The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the conservative sequence D,D,D,QXXRW.One full-length cDNA of the cellulose synthase-like protein D(C...The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the conservative sequence D,D,D,QXXRW.One full-length cDNA of the cellulose synthase-like protein D(CslD) gene was cloned by reverse transcriptase(RT)-polymerase chain reaction(PCR) with 5’,3’ rapid amplification of cDNA ends(RACE) methods using degenerate primers designed from the homologous sequences of the CesA genes.A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence.Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids.The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity (over 71%) with the ClCslD genes from Populus tremuloides,Oryza sativa,and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants.展开更多
Forest genetic maps are an important component within the forest biologist’s toolkit, underpinning forest improvement programs.The estimation of forest genetic maps is a conceptually simple yet computationally comple...Forest genetic maps are an important component within the forest biologist’s toolkit, underpinning forest improvement programs.The estimation of forest genetic maps is a conceptually simple yet computationally complex problem,due to long growth period and high heterozygosis and genetic load of forest trees.At present,some genetic maps of trees have been constructed.This paper reviewed the process of forest genetic map construction,highlighting its uniqueness,and pointed out the problems that might rise in mapping.Finally, prospect was made for constructing the high density genetic map of trees.展开更多
基金supported by the Natural Science Foundation of China (30800879)
文摘DNA methylation plays a crucial role in regulating plant development and tissue differentiation. In this study, we compared the methylation levels in leaf, root, and stem in Chamaedorea elegans by using the technique of methylation-sensitive amplified fragment length polymorphism AFLP. Over 19% （42/220） bases were uniformly methy- lated in these tissues. The percentages of polymorphism resulting from varied methylation in mature leaf （L1）, young leaf （L2）, baby leaf （L3）, stem （S）, young root （R1） and lignified root （R2） were 29.5%, 29.0%, 27.1%, 30.7%, 63.0% and 28.3%, respectively. The numbers of poly- morphic loci detected in the leaves of three developmental stages were similar, ranging from 20 to 30. In contrast, roots at the two developmental stages differed greatly, with 145 polymorphic loci detected in R1 and 27 in R2. Our results suggest that the methylation level in leaves slightly increases with aging, while that in roots decreases dramatically with aging.
文摘The protein structure of the cellulose synthase-like protein(CSL) was similar to cellulose synthase(CesA),including the conservative sequence D,D,D,QXXRW.One full-length cDNA of the cellulose synthase-like protein D(CslD) gene was cloned by reverse transcriptase(RT)-polymerase chain reaction(PCR) with 5’,3’ rapid amplification of cDNA ends(RACE) methods using degenerate primers designed from the homologous sequences of the CesA genes.A multiple comparison sequence analysis was conducted concurrently with bioinformatic methods to analyze the obtained sequence.Results of the sequence analysis showed that this cDNA was 4 150 bp in length and contained a single open reading frame encoding a protein of 1 132 amino acids.The multiple comparison sequence analysis showed that the deduced amino acid sequence shared high similarity (over 71%) with the ClCslD genes from Populus tremuloides,Oryza sativa,and Arabidopsis thaliana. This work will help lay an important foundation for further molecular studies with cellulose synthesis of plants.
文摘Forest genetic maps are an important component within the forest biologist’s toolkit, underpinning forest improvement programs.The estimation of forest genetic maps is a conceptually simple yet computationally complex problem,due to long growth period and high heterozygosis and genetic load of forest trees.At present,some genetic maps of trees have been constructed.This paper reviewed the process of forest genetic map construction,highlighting its uniqueness,and pointed out the problems that might rise in mapping.Finally, prospect was made for constructing the high density genetic map of trees.
