Pyropia yezoensis(P.yezoensis)is a popular species of red algae that are commercially cultivated and consumed in East Asia,China,Japan,and Korea.The high protein content of P.yezoensis provides a source of multiple bi...Pyropia yezoensis(P.yezoensis)is a popular species of red algae that are commercially cultivated and consumed in East Asia,China,Japan,and Korea.The high protein content of P.yezoensis provides a source of multiple bioactive peptides exhibiting antioxidant,anti-inflammatory,antihypertensive,anticancer,tissue healing,immunomodulatory,and anticoagulant properties.Furthermore,many other biologically active substances in P.yezoensis,including carbohydrates,lipids,dietary fibers,and polyphenols,have shown potential health benefits and are important in both the food and agriculture industries.This review provides a detailed summary of researches over the last decade on the biological and medicinal properties of bioactive peptides.The information was extracted from various electronic resources,including Google Scholar,PubMed,MEDLINE,and Google Patents.展开更多
We extracted a glycoprotein from the brown alga Laminaria japonica (LJGP). We previously demonstrated that LJGP induced apop- tosis in HT-29 colon cancer cells via the Fas- and the mitochondrial signaling pathway, and...We extracted a glycoprotein from the brown alga Laminaria japonica (LJGP). We previously demonstrated that LJGP induced apop- tosis in HT-29 colon cancer cells via the Fas- and the mitochondrial signaling pathway, and cell-cycle arrest. However, its effect on the cell membrane remained unknown. In this study, we identified the involvement of matrix metalloproteinase (MMP), integrin, and Epi- thelial (E)-cadherin in LJGP-induced apoptosis in HT-29 cells. LJGP treatment increased the expression and activity of MMP-2 and MMP-9. Furthermore, LJGP decreased the expression of integrin αν, β3, β5, β6 and E- cadherin. Consistent with a decreased expression of E-cadherin, LJGP inhibited the Wnt signaling pathway. Moreover, activation of downstream molecules of integrin, including focal adhesion kinase (FAK), the Src family of protooncogenic tyrosine kinases, extracellular signal-related kinase (ERK), and phosphatidyl inositol 3 kinase (PI-3K) were also decreased. These findings suggest that LJGP-induced apoptosis of HT-29 cells involves possible ECM disruption and cell detachment, which are executed principally through the activation of MMPs and by a decrease of adhesion molecules, contributing to a down-regulation of the PI-3K, MAPK, and Wnt signaling pathways. Apoptosis induced by ECM disruption or cell detachment is also known as anoikis. We can say that LJGP induces anoikis in HT-29 cells.展开更多
基金the Ministry of Education(Grant No.2012R1A6A1028677),supported this research.
文摘Pyropia yezoensis(P.yezoensis)is a popular species of red algae that are commercially cultivated and consumed in East Asia,China,Japan,and Korea.The high protein content of P.yezoensis provides a source of multiple bioactive peptides exhibiting antioxidant,anti-inflammatory,antihypertensive,anticancer,tissue healing,immunomodulatory,and anticoagulant properties.Furthermore,many other biologically active substances in P.yezoensis,including carbohydrates,lipids,dietary fibers,and polyphenols,have shown potential health benefits and are important in both the food and agriculture industries.This review provides a detailed summary of researches over the last decade on the biological and medicinal properties of bioactive peptides.The information was extracted from various electronic resources,including Google Scholar,PubMed,MEDLINE,and Google Patents.
文摘We extracted a glycoprotein from the brown alga Laminaria japonica (LJGP). We previously demonstrated that LJGP induced apop- tosis in HT-29 colon cancer cells via the Fas- and the mitochondrial signaling pathway, and cell-cycle arrest. However, its effect on the cell membrane remained unknown. In this study, we identified the involvement of matrix metalloproteinase (MMP), integrin, and Epi- thelial (E)-cadherin in LJGP-induced apoptosis in HT-29 cells. LJGP treatment increased the expression and activity of MMP-2 and MMP-9. Furthermore, LJGP decreased the expression of integrin αν, β3, β5, β6 and E- cadherin. Consistent with a decreased expression of E-cadherin, LJGP inhibited the Wnt signaling pathway. Moreover, activation of downstream molecules of integrin, including focal adhesion kinase (FAK), the Src family of protooncogenic tyrosine kinases, extracellular signal-related kinase (ERK), and phosphatidyl inositol 3 kinase (PI-3K) were also decreased. These findings suggest that LJGP-induced apoptosis of HT-29 cells involves possible ECM disruption and cell detachment, which are executed principally through the activation of MMPs and by a decrease of adhesion molecules, contributing to a down-regulation of the PI-3K, MAPK, and Wnt signaling pathways. Apoptosis induced by ECM disruption or cell detachment is also known as anoikis. We can say that LJGP induces anoikis in HT-29 cells.
