Viral diseases are among the most critical damaging factors that impose a global threat to the cucurbit industry.China is the world’s leading country for the production and consumption of cucurbits.Guangdong,a provin...Viral diseases are among the most critical damaging factors that impose a global threat to the cucurbit industry.China is the world’s leading country for the production and consumption of cucurbits.Guangdong,a province in southern China dominated by the tropical and subtropical climate,favors the survival of different plant viruses and their vectors.Five main cucurbit crops showing various disease symptoms were surveyed and collected to identify viruses infecting cucurbits in Guangdong during 2018–2020.In the field,the incidence ranged from 5-30%,or even 60-100% in the case of severely infected cucurbits.A total of 357 symptomatic samples were collected and subsequently screened for cucurbit viruses by small RNA deep sequencing and assembly(sRSA).Seventeen virus species belonging to 10 genera were identified in the five main cucurbit crops.The most common viruses were papaya ringspot virus(PRSV;Potyvirus),zucchini tigre mosaic virus(ZTMV;Potyvirus),zucchini yellow mosaic virus(ZYMV;Potyvirus),and watermelon silver mottle virus(WSMoV;Orthotospovirus),with infection rates of 24.4,19.0,17.1,and 14.3%,respectively.Notably,the most prevalent viruses were melon yellow spot orthotospovirus(MYSV)in cucumber,PRSV in squash,cucumber green mottle mosaic virus(CGMMV;Tobamovirus)in bottle gourd,WSMoV in white gourd,and ZYMV in luffa.Mixed infections were prevalent,and the types of mixed infections varied substantially in different cucurbit crops.Moreover,the full-length nucleotide sequences of watermelon green mottle mosaic virus(WGMMV),CGMMV,and watermelon virus A(WVA;Wamavirus)identified in bottle gourd were cloned and analyzed.This study is the first reporting WGMMV infecting bottle gourd in China mainland.In summary,the results demonstrate that in Guangdong,the most prevalent viruses belong to potyviruses,orthotospoviruses,and tobamoviruses groups.The findings will facilitate agricultural researchers and farmers to plan and implement effective disease control strategies aiming at timely detection and management of cucurbit-infecting viral pathogens.展开更多
The China-Pakistan Economic Corridor(CPEC)stands as a testament to the enduring friendship between Pakistan and China.It is a comprehensive development project that promises to usher in a new era of economic growth,in...The China-Pakistan Economic Corridor(CPEC)stands as a testament to the enduring friendship between Pakistan and China.It is a comprehensive development project that promises to usher in a new era of economic growth,infrastructure development and regional connectivity for Pakistan.Spanning across various sectors,the CPEC has the potential to positively change the socioeconomic landscape of Pakistan.展开更多
China has achieved tremendous progress in the last 72 years,something every nation may desire.The Chinese nation has struggled collectively to reach the highest level of progress and prosperity.It did not turn a keen ...China has achieved tremendous progress in the last 72 years,something every nation may desire.The Chinese nation has struggled collectively to reach the highest level of progress and prosperity.It did not turn a keen ear to what the others in the world were saying.展开更多
基金supported by the grants from the National Natural Science Foundation of China(31801712)the Key Research and Development Program of Guangdong Province,China(2018B020202006)+1 种基金the Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences(202103TD and 202105TD)the Development Program for Guangdong Province Modern Agricultural Science and Technology Innovation Alliance(2020KJ113)。
文摘Viral diseases are among the most critical damaging factors that impose a global threat to the cucurbit industry.China is the world’s leading country for the production and consumption of cucurbits.Guangdong,a province in southern China dominated by the tropical and subtropical climate,favors the survival of different plant viruses and their vectors.Five main cucurbit crops showing various disease symptoms were surveyed and collected to identify viruses infecting cucurbits in Guangdong during 2018–2020.In the field,the incidence ranged from 5-30%,or even 60-100% in the case of severely infected cucurbits.A total of 357 symptomatic samples were collected and subsequently screened for cucurbit viruses by small RNA deep sequencing and assembly(sRSA).Seventeen virus species belonging to 10 genera were identified in the five main cucurbit crops.The most common viruses were papaya ringspot virus(PRSV;Potyvirus),zucchini tigre mosaic virus(ZTMV;Potyvirus),zucchini yellow mosaic virus(ZYMV;Potyvirus),and watermelon silver mottle virus(WSMoV;Orthotospovirus),with infection rates of 24.4,19.0,17.1,and 14.3%,respectively.Notably,the most prevalent viruses were melon yellow spot orthotospovirus(MYSV)in cucumber,PRSV in squash,cucumber green mottle mosaic virus(CGMMV;Tobamovirus)in bottle gourd,WSMoV in white gourd,and ZYMV in luffa.Mixed infections were prevalent,and the types of mixed infections varied substantially in different cucurbit crops.Moreover,the full-length nucleotide sequences of watermelon green mottle mosaic virus(WGMMV),CGMMV,and watermelon virus A(WVA;Wamavirus)identified in bottle gourd were cloned and analyzed.This study is the first reporting WGMMV infecting bottle gourd in China mainland.In summary,the results demonstrate that in Guangdong,the most prevalent viruses belong to potyviruses,orthotospoviruses,and tobamoviruses groups.The findings will facilitate agricultural researchers and farmers to plan and implement effective disease control strategies aiming at timely detection and management of cucurbit-infecting viral pathogens.
文摘The China-Pakistan Economic Corridor(CPEC)stands as a testament to the enduring friendship between Pakistan and China.It is a comprehensive development project that promises to usher in a new era of economic growth,infrastructure development and regional connectivity for Pakistan.Spanning across various sectors,the CPEC has the potential to positively change the socioeconomic landscape of Pakistan.
文摘China has achieved tremendous progress in the last 72 years,something every nation may desire.The Chinese nation has struggled collectively to reach the highest level of progress and prosperity.It did not turn a keen ear to what the others in the world were saying.
