The projection pursuit model is used to study the assessment of air pollution caused by vehicle emissions at intersections. Based on the analysis of the characteristics and regularities of vehicle emissions at interse...The projection pursuit model is used to study the assessment of air pollution caused by vehicle emissions at intersections. Based on the analysis of the characteristics and regularities of vehicle emissions at intersections, a vehicle emission model based on projection pursuit is established, and the bat algorithm is used to solve the optimization function. The research results show that the projection pursuit model can not only measure the air pollution of vehicle emissions at intersections, but also effectively evaluate the level of vehicle exhaust emissions at intersections. Taking the air pollution caused by vehicle emissions at intersections as the research object and considering the influence factors of vehicle emissions on air pollution comprehensively, the evaluation index system of vehicle emissions at intersections on air pollution is constructed. Based on large data analysis, a prediction model of air pollution caused by vehicle emissions at intersections is constructed, and an improved bat algorithm is used to realize the assessment process. The application results show that the prediction model of vehicle emissions at intersections can define the degree of air pollution caused by vehicle emissions, and it has good guiding significance and practical value for solving the problem of air pollution caused by vehicle emissions.展开更多
The Gram-positive bacterium Staphylococcus aureus(S.aureus)is a wide spread common opportunistic pathogen that causes a wide variety of infectious diseases,from benign skin infections to life-threatening diseases such...The Gram-positive bacterium Staphylococcus aureus(S.aureus)is a wide spread common opportunistic pathogen that causes a wide variety of infectious diseases,from benign skin infections to life-threatening diseases such as the methicillin-resistant Staphylococcus aureus(MRSA)infection.Although emerging evidence suggests that lysine acetylation may play critical roles in bacterial physiology,the atlas of acetylome in S.aureus has not been studied.To comprehensively profile protein lysine acetylation in S.aureus,we used an integrated approach that combined immune affinity peptide enrichment using anti-lysine acetylation antibody,high-pH HPLC fractionation,and HPLC/mass spectrometry analysis.This study led to the identification of 1361 non-redundant acetylation sites on 412 proteins found in a search of S.aureus protein database extracted from the Swiss-Prot database.We further performed bioinformatic analysis to characterize this modification,including gene ontology annotation,protein-protein interaction,and domain analysis of the acetylation sites.We found that the acetylated proteins were enriched in multiple biological pathways,such as ribosomal function and energy metabolism.Our data provides a rich source for functional studies of lysine acetylation in S.aureus.展开更多
基金The National Natural Science Foundation of China(No.51178157)High-Level Project of the Top Six Talents in Jiangsu Province(No.JXQC-021)+1 种基金Key Science and Technology Program in Henan Province(No.182102310004)the Humanities and Social Science Research Programs Foundation of the M inistry of Education of China(No.18YJAZH028)
文摘The projection pursuit model is used to study the assessment of air pollution caused by vehicle emissions at intersections. Based on the analysis of the characteristics and regularities of vehicle emissions at intersections, a vehicle emission model based on projection pursuit is established, and the bat algorithm is used to solve the optimization function. The research results show that the projection pursuit model can not only measure the air pollution of vehicle emissions at intersections, but also effectively evaluate the level of vehicle exhaust emissions at intersections. Taking the air pollution caused by vehicle emissions at intersections as the research object and considering the influence factors of vehicle emissions on air pollution comprehensively, the evaluation index system of vehicle emissions at intersections on air pollution is constructed. Based on large data analysis, a prediction model of air pollution caused by vehicle emissions at intersections is constructed, and an improved bat algorithm is used to realize the assessment process. The application results show that the prediction model of vehicle emissions at intersections can define the degree of air pollution caused by vehicle emissions, and it has good guiding significance and practical value for solving the problem of air pollution caused by vehicle emissions.
基金supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China(2012ZX09301001-007)the National Natural Science Foundation of China(31370814)+1 种基金the Shanghai Pujiang Program(13PJ1410300)the support of China Postdoctoral Science Foundation(2013M531236,2013M541567)
文摘The Gram-positive bacterium Staphylococcus aureus(S.aureus)is a wide spread common opportunistic pathogen that causes a wide variety of infectious diseases,from benign skin infections to life-threatening diseases such as the methicillin-resistant Staphylococcus aureus(MRSA)infection.Although emerging evidence suggests that lysine acetylation may play critical roles in bacterial physiology,the atlas of acetylome in S.aureus has not been studied.To comprehensively profile protein lysine acetylation in S.aureus,we used an integrated approach that combined immune affinity peptide enrichment using anti-lysine acetylation antibody,high-pH HPLC fractionation,and HPLC/mass spectrometry analysis.This study led to the identification of 1361 non-redundant acetylation sites on 412 proteins found in a search of S.aureus protein database extracted from the Swiss-Prot database.We further performed bioinformatic analysis to characterize this modification,including gene ontology annotation,protein-protein interaction,and domain analysis of the acetylation sites.We found that the acetylated proteins were enriched in multiple biological pathways,such as ribosomal function and energy metabolism.Our data provides a rich source for functional studies of lysine acetylation in S.aureus.
