The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Bot...The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Both high quality microtuber and synchronizing microtuber which have the same size were necessary for producing health potato artificial seeds. In our experiment, the optimum medium of adventitious shoot-inducing of potato Hutao, Kesi, and Favorita is DCR + 1.0 mg / kg BA+ 0.5 mg / kg IBA+ 500 mg/ kg LH; the optium medium of microtuber- inducing of Hutao and Kesi is DCR+5.0 mg/ kg BA+200 mg/ kg Lh+8% sugar, and the optium medium of microtuber -inducing of Favorita is DCR+ 5.0 mg/ kg BA+0.1 mg / kg NAA+200 mg/ kg LH+8% sugar. Microtubers of the same size were gained by selecting the synchronous microtubers. 2.0% sodium alginate was used to encapsulate the microtubers of potato to form the artificial seeds, and the concentration of Ca C12 is 4%. Phytohormone NAA promoted the germination of potato artificial seeds. These artificial seeds have more than 90% germinating frequency in plant hormone-free DCR medium.展开更多
文摘The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Both high quality microtuber and synchronizing microtuber which have the same size were necessary for producing health potato artificial seeds. In our experiment, the optimum medium of adventitious shoot-inducing of potato Hutao, Kesi, and Favorita is DCR + 1.0 mg / kg BA+ 0.5 mg / kg IBA+ 500 mg/ kg LH; the optium medium of microtuber- inducing of Hutao and Kesi is DCR+5.0 mg/ kg BA+200 mg/ kg Lh+8% sugar, and the optium medium of microtuber -inducing of Favorita is DCR+ 5.0 mg/ kg BA+0.1 mg / kg NAA+200 mg/ kg LH+8% sugar. Microtubers of the same size were gained by selecting the synchronous microtubers. 2.0% sodium alginate was used to encapsulate the microtubers of potato to form the artificial seeds, and the concentration of Ca C12 is 4%. Phytohormone NAA promoted the germination of potato artificial seeds. These artificial seeds have more than 90% germinating frequency in plant hormone-free DCR medium.
