Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem （iPS） cells from mouse mature adipocytes by forced expression of six transcription...Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem （iPS） cells from mouse mature adipocytes by forced expression of six transcription factors （Oct4, Sox2, c-Myc, Klf4, Rarγ, and Lrh1） with a piggyBac transposon-based strategy. The resulting iPS cells were pluripotent as evidenced by the fact that they stained positive for alkaline phosphatase, expressed high levels of key pluripotency markers including Oct4, Nanog, and SSEA1, and remained pluripotent on a 2i media. In vitro differen- tiation of the iPS cells showed that the cell derivatives of all three germ layers could be readily obtained through forma- tion of embryoid bodies. Most importantly, these adipocyte- derived iPS cells were capable of producing chimera with high frequencies when reintroduced into early-stage em- bryos and transmitted through the germ line. This study demonstrates that the new six-factor reprogramming tech- nology facilitates the reset of the terminally differentiated adipocytes to the ground state of pluripotency, enabling us to fully explore the potential of mature adipocytes as a viable cell source for regenerative medicine.展开更多
A novel graphene oxide doped poly（hydroxymethylated-3,4-ethylenedioxythiophene） （PEDOT-MeOH/GO） composite film was synthesized and utilized as an efficient electrode material for simultaneous detection ofrutin and...A novel graphene oxide doped poly（hydroxymethylated-3,4-ethylenedioxythiophene） （PEDOT-MeOH/GO） composite film was synthesized and utilized as an efficient electrode material for simultaneous detection ofrutin and ascorbic acid （AA）. PEDOT-MeOH/GO films were synthesized on glassy carbon electrode （GCE） by a facile one-step electrochemical approach and were characterized by scanning electron microscopy, UV-Vis spectroscopy, FTIR spectra and electrochemical methods. Then the PEDOT-MeOH/GO/GCE was applied successfully in the simultaneous detection of rutin and AA. The results showed that the oxidation peak currents of rutin and AA obtained at the PEDOT-MeOH/GO/GCE were much higher than those at the traditional conducting polymer PEDOT/GO/GCE, PEDOT-MeOH/GCE, PEDOT/GCE and bare GCE. Under optimized conditions, the linear ranges for rutin and AA are 20 nmol/L-10 p.mol/L and 8 μmol/L-1 mmol/L, respectively. The detection limit is 6 nmol/L for rutin and 2 μmol/L for AA （S/N = 3）, which are lower than those of the reported electrochemical sensors.展开更多
基金supported in part by the National Basic Research Program of China(2011CB504004,2010CB945500)the Strategic Program of Stem Cell(XDA01020303)+1 种基金the Knowledge Innovation Program of the Chinese Academy of Sciencesthe National Natural Science Foundation of China(90813033)as well as The Guangdong Key High-end Foreign Experts Program Fund
文摘Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem （iPS） cells from mouse mature adipocytes by forced expression of six transcription factors （Oct4, Sox2, c-Myc, Klf4, Rarγ, and Lrh1） with a piggyBac transposon-based strategy. The resulting iPS cells were pluripotent as evidenced by the fact that they stained positive for alkaline phosphatase, expressed high levels of key pluripotency markers including Oct4, Nanog, and SSEA1, and remained pluripotent on a 2i media. In vitro differen- tiation of the iPS cells showed that the cell derivatives of all three germ layers could be readily obtained through forma- tion of embryoid bodies. Most importantly, these adipocyte- derived iPS cells were capable of producing chimera with high frequencies when reintroduced into early-stage em- bryos and transmitted through the germ line. This study demonstrates that the new six-factor reprogramming tech- nology facilitates the reset of the terminally differentiated adipocytes to the ground state of pluripotency, enabling us to fully explore the potential of mature adipocytes as a viable cell source for regenerative medicine.
基金financially supported by the National Natural Science Foundation of China(Nos.50963002,51073074,51272906,51263010 and 51302117)Natural Science Foundation of Jiangxi Province(Nos.2010GZH0041,20122BAB213007)+1 种基金Jiangxi Provincial Department of Education(Nos.GJJ11590,GJJ10678 and GJJ13258)Jiangxi Science&Technology Normal University Innovation Fund of Postgraduates(YC2013-X25)
文摘A novel graphene oxide doped poly（hydroxymethylated-3,4-ethylenedioxythiophene） （PEDOT-MeOH/GO） composite film was synthesized and utilized as an efficient electrode material for simultaneous detection ofrutin and ascorbic acid （AA）. PEDOT-MeOH/GO films were synthesized on glassy carbon electrode （GCE） by a facile one-step electrochemical approach and were characterized by scanning electron microscopy, UV-Vis spectroscopy, FTIR spectra and electrochemical methods. Then the PEDOT-MeOH/GO/GCE was applied successfully in the simultaneous detection of rutin and AA. The results showed that the oxidation peak currents of rutin and AA obtained at the PEDOT-MeOH/GO/GCE were much higher than those at the traditional conducting polymer PEDOT/GO/GCE, PEDOT-MeOH/GCE, PEDOT/GCE and bare GCE. Under optimized conditions, the linear ranges for rutin and AA are 20 nmol/L-10 p.mol/L and 8 μmol/L-1 mmol/L, respectively. The detection limit is 6 nmol/L for rutin and 2 μmol/L for AA （S/N = 3）, which are lower than those of the reported electrochemical sensors.
