Nyquist Folding Receiver(NYFR)is a perceptron structure that realizes a low probability of intercept(LPI)signal analog to information.Aiming at the problem of LPI radar signal receiving,the time domain,frequency domai...Nyquist Folding Receiver(NYFR)is a perceptron structure that realizes a low probability of intercept(LPI)signal analog to information.Aiming at the problem of LPI radar signal receiving,the time domain,frequency domain,and time-frequency domain problems of signals intercepted by NYFR structure are studied.Combined with the time-frequency analysis(TFA)method,a radar recognition scheme based on deep learning(DL)is introduced,which can reliably classify common LPI radar signals.First,the structure of NYFR and its characteristics in the time domain,frequency domain,and time and frequency domain are analyzed.Then,the received signal is then converted into a time-frequency image(TFI).Finally,four kinds of DL algorithms are used to classify LPI radar signals.Simulation results demonstrate the correctness of the NYFR structure,and the effectiveness of the proposed recognition method is verified by comparison experiments.展开更多
The development of wearable electronic systems has generated increasing demand for flexible power sources.Alkaline zinc(Zn)-based batteries,as one of the most mature energy storage technologies,have been considered as...The development of wearable electronic systems has generated increasing demand for flexible power sources.Alkaline zinc(Zn)-based batteries,as one of the most mature energy storage technologies,have been considered as a promising power source owing to their exceptional safety,low costs,and outstanding electrochemical performance.However,the conventional alkaline Zn-based battery systems face many challenges associated with electrodes and electrolytes,causing low capacity,poor cycle life,and inferior mechanical performance.Recent advances in materials and structure design have enabled the revisitation of the alkaline Zn-based battery technology for applications in flexible electronics.Herein,we summarize the up-to-date works in flexible alkaline Zn-based batteries and analyze the strategies employed to improve battery performance.Firstly,we introduce the three most reported cathode materials(including Ag-based,Ni-based,and Co-based materials)for flexible alkaline Zn-based batteries.Then,challenges and modifications in battery anodes are investigated.Thirdly,the recently advanced gel electrolytes are introduced from their properties,functions as well as advanced fabrications.Finally,recent works and the advantages of sandwich-type,fiber-type and thin film-type flexible batteries are summarized and compared.This review provides insights and guidance for the design of high-performance flexible Zn-based batteries for next-generation electronics.展开更多
AIM To analyze the risk factors for pancreatic fistula after pancreaticoduodenectomy.METHODS We conducted a retrospective analysis of 539 successive cases of pancreaticoduodenectomy performed at our hospital from Marc...AIM To analyze the risk factors for pancreatic fistula after pancreaticoduodenectomy.METHODS We conducted a retrospective analysis of 539 successive cases of pancreaticoduodenectomy performed at our hospital from March 2012 to October 2015. Pancreatic fistula was diagnosed in strict accordance with the definition of pancreatic fistula from the International Study Group on Pancreatic Fistula. The risk factors for pancreatic fistula were analyzed by univariate analysis and multivariate logistic regression analysis.RESULTS A total of 269(49.9%) cases of pancreatic fistula occurred after pancreaticoduodenectomy,including 71(13.17%) cases of grade A pancreatic fistula,178(33.02%) cases of grade B,and 20(3.71%) cases of grade C. Univariate analysis showed no significant correlation between postoperative pancreatic fistula(POPF) and the following factors: age,hypertension,alcohol consumption,smoking,history of upper abdominal surgery,preoperative jaundice management,preoperative bilirubin,preoperative albumin,pancreatic duct drainage,intraoperative blood loss,operative time,intraoperative blood transfusion,Braun anastomosis,and pancreaticoduodenectomy(with or without pylorus preservation). Conversely,a significant correlation was observed between POPF and the following factors: gender(male vs female: 54.23% vs 42.35%,P = 0.008),diabetes(non-diabetic vs diabetic: 51.61% vs 39.19%,P = 0.047),body mass index(BMI)(≤ 25 vs > 25: 46.94% vs 57.82%,P = 0.024),blood glucose level(≤ 6.0 mmol/L vs > 6.0 mmol/L: 54.75% vs 41.14%,P = 0.002),pancreaticojejunal anastomosis technique(pancreatic duct-jejunum double-layer mucosa-to-mucosa pancreaticojejunal anastomosis vs pancreatic-jejunum single-layer mucosa-tomucosa anastomosis: 57.54% vs 35.46%,P = 0.000),diameter of the pancreatic duct(≤ 3 mm vs > 3 mm: 57.81% vs 38.36%,P = 0.000),and pancreatic texture(soft vs hard: 56.72% vs 29.93%,P = 0.000). Multivariate logistic regression analysis showed that gender(male),BMI > 25,pancreatic duct-jejunum double-layer mucosa-to-mucosa pancreaticojejunal anastomosis,pancreatic duct diameter ≤ 3 mm,and soft pancreas were risk factors for pancreatic fistula after pancreaticoduodenectomy.CONCLUSION Gender(male),BMI > 25,pancreatic duct-jejunum double-layer mucosa-to-mucosa pancreaticojejunal anastomosis,pancreatic duct diameter ≤ 3 mm,and soft pancreas were risk factors for pancreatic fistula after pancreaticoduodenectomy.展开更多
AIM:To explore the morbidity and risk factors of postoperative pancreatic fistula(POPF)following pancreaticoduodenectomy.METHODS:The data from 196 consecutive patients who underwent pancreaticoduodenectomy,performed b...AIM:To explore the morbidity and risk factors of postoperative pancreatic fistula(POPF)following pancreaticoduodenectomy.METHODS:The data from 196 consecutive patients who underwent pancreaticoduodenectomy,performed by different surgeons,in the General Hospital of the People’s Liberation Army between January 1st,2013and December 31st,2013 were retrospectively collected for analysis.The diagnoses of POPF and clinically relevant(CR)-POPF following pancreaticoduodenectomy were judged strictly by the International Study Group on Pancreatic Fistula Definition.Univariate analysis was performed to analyze the following factors:patient age,sex,body mass index(BMI),hypertension,diabetes mellitus,serum CA19-9 level,history of jaundice,serum albumin level,blood loss volume,pancreatic duct diameter,pylorus preserving pancreaticoduodenectomy,pancreatic drainage and pancreaticojejunostomy.Multivariate logistic regression analysis was used to determine the main independent risk factors for POPF.RESULTS:POPF occurred in 126(64.3%)of the patients,and the incidence of CR-POPF was 32.7%(64/196).Patient characteristics of age,sex,BMI,hypertension,diabetes mellitus,serum CA19-9 level,history of jaundice,serum albumin level,blood loss volume,pylorus preserving pancreaticoduodenectomy and pancreaticojejunostomy showed no statistical difference related to the morbidity of POPF or CR-POPF.Pancreatic duct diameter was found to be significantly correlated with POPF rates by univariate analysis and multivariate regression analysis,with a pancreatic duct diameter≤3 mm being an independent risk factor for POPF(OR=0.291;P=0.000)and CR-POPF(OR=0.399;P=0.004).The CR-POPF rate was higher in patients without external pancreatic stenting,which was found to be an independent risk factor for CR-POPF(OR=0.394;P=0.012).Among the entire patient series,there were three postoperative deaths,giving a total mortality rate of 1.5%(3/196),and the mortality associated with pancreatic fistula was 2.4%(3/126).CONCLUSION:A pancreatic duct diameter≤3 mm is an independent risk factor for POPF.External stent drainage of pancreatic secretion may reduce CR-POPF mortality and POPF severity.展开更多
Living gymnosperms comprise four major groups:cycads,Ginkgo,conifers,and gnetophytes.Relationships among/within these lineages have not been fully resolved.Next generation sequencing has made available a large number ...Living gymnosperms comprise four major groups:cycads,Ginkgo,conifers,and gnetophytes.Relationships among/within these lineages have not been fully resolved.Next generation sequencing has made available a large number of sequences,including both plastomes and single-copy nuclear genes,for reconstruction of solid phylogenetic trees.Recent advances in gymnosperm phylogenomic studies have updated our knowledge of gymnosperm systematics.Here,we review major advances of gymnosperm phylogeny over the past 10 years and propose an updated classification of extant gymnosperms.This new classification includes three classes(Cycadopsida,Ginkgoopsida,and Pinopsida),five subclasses(Cycadidae,Ginkgoidae,Cupressidae,Pinidae,and Gnetidae),eight orders(Cycadales,Ginkgoales,Araucariales,Cupressales,Pinales,Ephedrales,Gnetales,and Welwitschiales),13 families,and 86 genera.We also described six new tribes including Acmopyleae Y.Yang,Austrocedreae Y.Yang,Chamaecyparideae Y.Yang,Microcachrydeae Y.Yang,Papuacedreae Y.Yang,and Prumnopityeae Y.Yang,and made 27 new combinations in the genus Sabina.展开更多
Background: Hepatic angiosarcoma is a rare malignant vascular tumor presenting unique treatment challenges.The aim of the present study was to determine the treatment and prognosis of this entity.Data sources: A sys...Background: Hepatic angiosarcoma is a rare malignant vascular tumor presenting unique treatment challenges.The aim of the present study was to determine the treatment and prognosis of this entity.Data sources: A systematic literature search was conducted using PubMed, Embase and Chinese BiomedicalLiterature database, to identify articles published from January 1980 to July 2017. Search termswere “hepatic angiosarcoma” and “liver angiosarcoma”. Additional articles were retrieved through manualsearch of bibliographies of the relevant articles. Pooled individual data concerning the prognosis followingvarious therapeutic modalities were analyzed.展开更多
AIM:To observe the curative effect of galactosylated chitosan(GC)/5-fluorouracil(5-FU) nanoparticles in liver caner mice and its side effects.METHODS:The GC/5-FU nanoparticle is a nanomaterial made by coupling GC and ...AIM:To observe the curative effect of galactosylated chitosan(GC)/5-fluorouracil(5-FU) nanoparticles in liver caner mice and its side effects.METHODS:The GC/5-FU nanoparticle is a nanomaterial made by coupling GC and 5-FU.The release experiment was performed in vitro.The orthotropic liver cancer mouse models were established and divided into control,GC,5-FU and GC/5-FU groups.Mice in the control and GC group received an intravenous injection of 200 L saline and GC,respectively.Mice in the 5-FU and GC/5-FU groups received 200 L(containing 0.371 mg 5-FU) 5-FU and GC/5-FU,respectively.The tumor weight and survival time were observed.The cell cycle and apoptosis in tumor tissues were monitored by flow cytometry.The expression of p53,Bax,Bcl-2,caspase-3 and poly adenosine 50-diphosphate-ribose polymerase 1(PARP-1) was detected by immunohistochemistry,reverse transcription-polymerase chain reaction and Western blot.The serum blood biochemical parameters and cytotoxic activity of natural killer(NK) cell and cytotoxicity T lymphocyte(CTL) were measured.RESULTS:The GC/5-FU nanoparticle is a sustained release system.The drug loading was 6.12% ± 1.36%,the encapsulation efficiency was 81.82% ± 5.32%,and the Zeta potential was 10.34 ± 1.43 mV.The tumor weight in the GC/5-FU group(0.4361 ± 0.1153 g vs 1.5801 ± 0.2821 g,P < 0.001) and the 5-FU(0.7932 ± 0.1283 g vs 1.5801 ± 0.2821 g,P < 0.001) was significantly lower than that in the control group;GC/5FU treatment can significantly lower the tumor weight(0.4361 ± 0.1153 g vs 0.7932 ± 0.1283 g,P < 0.001),and the longest median survival time was seen in the GC/5-FU group,compared with the control(12 d vs 30 d,P < 0.001),GC(13 d vs 30 d,P < 0.001) and 5-FU groups(17 d vs 30 d,P < 0.001).Flow cytometry revealed that compared with the control,GC/5FU caused a higher rate of G0-G1 arrest(52.79% ± 13.42% vs 23.92% ± 9.09%,P = 0.014) and apoptosis(2.55% ± 1.10% vs 11.13% ± 11.73%,P < 0.001) in hepatic cancer cells.Analysis of the apoptosis pathways showed that GC/5-FU upregulated the expression of p53 at both the protein and the mRNA levels,which in turn lowered the ratio of Bcl-2/Bax expression;this led to the release of cytochrome C into the cytosol from the mitochondria and the subsequent activation of caspase-3.Upregulation of caspase-3 expression decreased the PARP-1 at both the mRNA and the protein levels,which contributed to apoptosis.5-FU increased the levels of aspartate aminotransferase and alanine aminotransferase,and decreased the numbers of platelet,white blood cell and lymphocyte and cytotoxic activities of CTL and NK cells,however,there were no such side effects in the GC/5-FU group.CONCLUSION:GC/5-FU nanoparticles can significantly inhibit the growth of liver cancer in mice via the p53 apoptosis pathway,and relieve the side effects and immunosuppression of 5-FU.展开更多
Six main mitochondrial DNA (mtDNA) lineages have been described in minnow (Zacco platypus) samples obtained from northern, western and southern China Perdices et al. (2004) predicted that further sampling of oth...Six main mitochondrial DNA (mtDNA) lineages have been described in minnow (Zacco platypus) samples obtained from northern, western and southern China Perdices et al. (2004) predicted that further sampling of other tributaries might discover more lineages of this species. In this study, we collected 26 Zacco platypus individuals in the Huangshan area of eastern China and determined the cytochrome b (cytb) sequence variations. Combined with reported data in GenBank, we identified ten matrilines (Zacco A-J) in a total of 169 samples, with relatively high molecular divergence found among them. The Huangshan population had the greatest genetic variation among all sampled regions and hosted six of the ten matrilines. Our results highlight the significance of the Huangshan area for the conservation of Zacco platypus.展开更多
With shale oil reservoir pressure depletion and recovery of hydrocarbons from formations, the fracture apertures and conductivity are subject to reduction due to the interaction between stress effects and proppants. S...With shale oil reservoir pressure depletion and recovery of hydrocarbons from formations, the fracture apertures and conductivity are subject to reduction due to the interaction between stress effects and proppants. Suppose most of the proppants were concentrated in dominant fractures rather than sparsely allocated in the fracture network, the fracture conductivity would be less influenced by the induced stress effect. However, the merit of uniformly distributed proppants in the fracture network is that it increases the contact area for the injection gas with the shale matrix. In this paper, we address the question whether we should exploit or confine the fracture complexity for CO2-EOR in shale oil reservoirs. Two proppant transport scenarios were simulated in this paper: Case 1-the proppant is uniformly distributed in the complex fracture system, propagating a partially propped or un-propped fracture network; Case 2-the proppant primarily settles in simple planar fractures. A series of sensitivity studies of the fracture conductivity were performed to investigate the conductivity requirements in order to more efficiently produce from the shale reservoirs. Our simulation results in this paper show the potential of CO2 huff-n-puff to improve oil recovery in shale oil reservoirs. Simulation results indicate that the ultra-low permeability shales require an interconnected fracture network to maximize shale oil recovery in a reasonable time period. The well productivity of a fracture network with a conductivity of 4 mD ft achieves a better performance than that of planar fractures with an infinite conductivity. However, when the conductivity of fracture networks is inadequate,the planar fracture treatment design maybe a favorable choice. The available literature provides limited information on the relationship between fracture treatment design and the applicability of CO2 huff-n-puff in very low permeability shale formations. Very limited field test or laboratory data are available on the investigation of conductivity requirements for cyclic CO2 injection in shale oil reservoirs. In the context of CO2 huff-n-puff EOR, the effect of fracture complexity on well productivity was examined by simulation approaches.展开更多
The effects of postthermal treatment and irradiation time on the structure and thermal stability of TiO2/polyacrylate nanocomposites by a sol-gel process in reverse micelles and subsequent rapid photopolymerization we...The effects of postthermal treatment and irradiation time on the structure and thermal stability of TiO2/polyacrylate nanocomposites by a sol-gel process in reverse micelles and subsequent rapid photopolymerization were investigated, and the hybrid films were characterized by thermal gravimetry analysis (TGA), X-ray photoelectron spectrum (XPS), and atomic force microscopy (AFM). XPS data suggested that the prolongation of irradiation time and the postthermal treatment promoted titania formation, with the former affecting more remarkably. TGA data showed that TiO2-hybrid films could upgrade the decomposition onset temperature (Tonset) as well as the temperature at which there is a maximum mass loss rate (Tmax). AFM data demonstrated that the inorganic titania particles with a mean diameter of 25.26-28.84 nm were homogeneously distributed in the organic matrix.展开更多
AIM: To investigate the simplicity, reliability, andsafety of the application of single-layer mucosa-tomucosa pancreaticojejunal anastomosis in pancreaticoduodenectomy. METHODS: A retrospective analysis was performed ...AIM: To investigate the simplicity, reliability, andsafety of the application of single-layer mucosa-tomucosa pancreaticojejunal anastomosis in pancreaticoduodenectomy. METHODS: A retrospective analysis was performed on the data of patients who received pancreaticoduodenectomy completed by the same surgical group between January 2011 and April 2014 in the General Hospital of the People's Liberation Army. In total, 51 cases received single-layer mucosa-to-mucosa pancreaticojejunal anastomosis and 51 cases received double-layer pancreaticojejunal anastomosis. The diagnoses of pancreatic fistula and clinically relevant pancreatic fistula after pancreaticoduodenectomy were judged strictly by the International Study Group on pancreatic fistula definition. The preoperative and intraoperative data of these two groups were compared. χ2 test and Fisher's exact test were used to analyze the incidences of pancreatic fistula, peritoneal catheterization, abdominal infection and overall complications between the single-layer anastomosis group and double-layer anastomosis group. Rank sum test were used to analyze the difference in operation time, pancreaticojejunal anastomosis time, postoperative hospitalization time, total hospitalization time and hospitalization expenses between the single-layer anastomosis group and double-layer anastomosis group.RESULTS: Patients with grade A pancreatic fistula accounted for 15.69%(8/51) vs 15.69%(8/51)(P = 1.0000), and patients with grades B and C pancreatic fistula accounted for 9.80%(5/51) vs 52.94%(27/51)(P = 0.0000) in the single-layer and double-layer anastomosis groups. Although there was no significant difference in the percentage of patients with grade A pancreatic fistula, there was a significant difference in the percentage of patients with grades B and C pancreatic fistula between the two groups. Theoperation time(220.059 ± 60.602 min vs 379.412 ± 90.761 min, P = 0.000), pancreaticojejunal anastomosis time(17.922 ± 5.145 min vs 31.333 ± 7.776 min, P = 0.000), postoperative hospitalization time(18.588 ± 5.285 d vs 26.373 ± 15.815 d, P = 0.003), total hospitalization time(25.627 ± 6.551 d vs 33.706 ± 15.899 d, P = 0.002), hospitalization expenses(116787.667 ± 31900.927 yuan vs 162788.608 ± 129732.500 yuan, P = 0.001), as well as the incidences of pancreatic fistula [13/51(25.49%) vs 35/51(68.63%), P = 0.0000], peritoneal catheterization [0/51(0%) vs 6/51(11.76%), P = 0.0354], abdominal infection [1/51(1.96%) vs 11/51(21.57%), P = 0.0021], and overall complications [21/51(41.18%) vs 37/51(72.55%), P = 0.0014] in the single-layer anastomosis group were all lower than those in the double-layer anastomosis group. CONCLUSION: Single-layer mucosa-to-mucosa pancreaticojejunal anastomosis appears to be a simple, reliable, and safe method. Use of this method could reduce the postoperative incidence of complications.展开更多
Ginger(Zingiber officinale),the type species of Zingiberaceae,is one of the most widespread medicinal plants and spices.Here,we report a high-quality,chromosome-scale reference genome of ginger‘Zhugen’,a traditional...Ginger(Zingiber officinale),the type species of Zingiberaceae,is one of the most widespread medicinal plants and spices.Here,we report a high-quality,chromosome-scale reference genome of ginger‘Zhugen’,a traditionally cultivated ginger in Southwest China used as a fresh vegetable,assembled from PacBio long reads,Illumina short reads,and high-throughput chromosome conformation capture(Hi-C)reads.The ginger genome was phased into two haplotypes,haplotype 1(1.53 Gb with a contig N50 of 4.68 M)and haplotype 0(1.51 Gb with a contig N50 of 5.28 M).Homologous ginger chromosomes maintained excellent gene pair collinearity.In 17,226 pairs of allelic genes,11.9%exhibited differential expression between alleles.Based on the results of ginger genome sequencing,transcriptome analysis,and metabolomic analysis,we proposed a backbone biosynthetic pathway of gingerol analogs,which consists of 12 enzymatic gene families,PAL,C4H,4CL,CST,C3’H,C3OMT,CCOMT,CSE,PKS,AOR,DHN,and DHT.These analyses also identified the likely transcription factor networks that regulate the synthesis of gingerol analogs.Overall,this study serves as an excellent resource for further research on ginger biology and breeding,lays a foundation for a better understanding of ginger evolution,and presents an intact biosynthetic pathway for species-specific gingerol biosynthesis.展开更多
基金supported by the National Defence Pre-research Foundation of China。
文摘Nyquist Folding Receiver(NYFR)is a perceptron structure that realizes a low probability of intercept(LPI)signal analog to information.Aiming at the problem of LPI radar signal receiving,the time domain,frequency domain,and time-frequency domain problems of signals intercepted by NYFR structure are studied.Combined with the time-frequency analysis(TFA)method,a radar recognition scheme based on deep learning(DL)is introduced,which can reliably classify common LPI radar signals.First,the structure of NYFR and its characteristics in the time domain,frequency domain,and time and frequency domain are analyzed.Then,the received signal is then converted into a time-frequency image(TFI).Finally,four kinds of DL algorithms are used to classify LPI radar signals.Simulation results demonstrate the correctness of the NYFR structure,and the effectiveness of the proposed recognition method is verified by comparison experiments.
基金financial support from the Australian Research Council(LP1900113)。
文摘The development of wearable electronic systems has generated increasing demand for flexible power sources.Alkaline zinc(Zn)-based batteries,as one of the most mature energy storage technologies,have been considered as a promising power source owing to their exceptional safety,low costs,and outstanding electrochemical performance.However,the conventional alkaline Zn-based battery systems face many challenges associated with electrodes and electrolytes,causing low capacity,poor cycle life,and inferior mechanical performance.Recent advances in materials and structure design have enabled the revisitation of the alkaline Zn-based battery technology for applications in flexible electronics.Herein,we summarize the up-to-date works in flexible alkaline Zn-based batteries and analyze the strategies employed to improve battery performance.Firstly,we introduce the three most reported cathode materials(including Ag-based,Ni-based,and Co-based materials)for flexible alkaline Zn-based batteries.Then,challenges and modifications in battery anodes are investigated.Thirdly,the recently advanced gel electrolytes are introduced from their properties,functions as well as advanced fabrications.Finally,recent works and the advantages of sandwich-type,fiber-type and thin film-type flexible batteries are summarized and compared.This review provides insights and guidance for the design of high-performance flexible Zn-based batteries for next-generation electronics.
文摘AIM To analyze the risk factors for pancreatic fistula after pancreaticoduodenectomy.METHODS We conducted a retrospective analysis of 539 successive cases of pancreaticoduodenectomy performed at our hospital from March 2012 to October 2015. Pancreatic fistula was diagnosed in strict accordance with the definition of pancreatic fistula from the International Study Group on Pancreatic Fistula. The risk factors for pancreatic fistula were analyzed by univariate analysis and multivariate logistic regression analysis.RESULTS A total of 269(49.9%) cases of pancreatic fistula occurred after pancreaticoduodenectomy,including 71(13.17%) cases of grade A pancreatic fistula,178(33.02%) cases of grade B,and 20(3.71%) cases of grade C. Univariate analysis showed no significant correlation between postoperative pancreatic fistula(POPF) and the following factors: age,hypertension,alcohol consumption,smoking,history of upper abdominal surgery,preoperative jaundice management,preoperative bilirubin,preoperative albumin,pancreatic duct drainage,intraoperative blood loss,operative time,intraoperative blood transfusion,Braun anastomosis,and pancreaticoduodenectomy(with or without pylorus preservation). Conversely,a significant correlation was observed between POPF and the following factors: gender(male vs female: 54.23% vs 42.35%,P = 0.008),diabetes(non-diabetic vs diabetic: 51.61% vs 39.19%,P = 0.047),body mass index(BMI)(≤ 25 vs > 25: 46.94% vs 57.82%,P = 0.024),blood glucose level(≤ 6.0 mmol/L vs > 6.0 mmol/L: 54.75% vs 41.14%,P = 0.002),pancreaticojejunal anastomosis technique(pancreatic duct-jejunum double-layer mucosa-to-mucosa pancreaticojejunal anastomosis vs pancreatic-jejunum single-layer mucosa-tomucosa anastomosis: 57.54% vs 35.46%,P = 0.000),diameter of the pancreatic duct(≤ 3 mm vs > 3 mm: 57.81% vs 38.36%,P = 0.000),and pancreatic texture(soft vs hard: 56.72% vs 29.93%,P = 0.000). Multivariate logistic regression analysis showed that gender(male),BMI > 25,pancreatic duct-jejunum double-layer mucosa-to-mucosa pancreaticojejunal anastomosis,pancreatic duct diameter ≤ 3 mm,and soft pancreas were risk factors for pancreatic fistula after pancreaticoduodenectomy.CONCLUSION Gender(male),BMI > 25,pancreatic duct-jejunum double-layer mucosa-to-mucosa pancreaticojejunal anastomosis,pancreatic duct diameter ≤ 3 mm,and soft pancreas were risk factors for pancreatic fistula after pancreaticoduodenectomy.
文摘AIM:To explore the morbidity and risk factors of postoperative pancreatic fistula(POPF)following pancreaticoduodenectomy.METHODS:The data from 196 consecutive patients who underwent pancreaticoduodenectomy,performed by different surgeons,in the General Hospital of the People’s Liberation Army between January 1st,2013and December 31st,2013 were retrospectively collected for analysis.The diagnoses of POPF and clinically relevant(CR)-POPF following pancreaticoduodenectomy were judged strictly by the International Study Group on Pancreatic Fistula Definition.Univariate analysis was performed to analyze the following factors:patient age,sex,body mass index(BMI),hypertension,diabetes mellitus,serum CA19-9 level,history of jaundice,serum albumin level,blood loss volume,pancreatic duct diameter,pylorus preserving pancreaticoduodenectomy,pancreatic drainage and pancreaticojejunostomy.Multivariate logistic regression analysis was used to determine the main independent risk factors for POPF.RESULTS:POPF occurred in 126(64.3%)of the patients,and the incidence of CR-POPF was 32.7%(64/196).Patient characteristics of age,sex,BMI,hypertension,diabetes mellitus,serum CA19-9 level,history of jaundice,serum albumin level,blood loss volume,pylorus preserving pancreaticoduodenectomy and pancreaticojejunostomy showed no statistical difference related to the morbidity of POPF or CR-POPF.Pancreatic duct diameter was found to be significantly correlated with POPF rates by univariate analysis and multivariate regression analysis,with a pancreatic duct diameter≤3 mm being an independent risk factor for POPF(OR=0.291;P=0.000)and CR-POPF(OR=0.399;P=0.004).The CR-POPF rate was higher in patients without external pancreatic stenting,which was found to be an independent risk factor for CR-POPF(OR=0.394;P=0.012).Among the entire patient series,there were three postoperative deaths,giving a total mortality rate of 1.5%(3/196),and the mortality associated with pancreatic fistula was 2.4%(3/126).CONCLUSION:A pancreatic duct diameter≤3 mm is an independent risk factor for POPF.External stent drainage of pancreatic secretion may reduce CR-POPF mortality and POPF severity.
基金supported by the National Natural Science Foundation of China(31970205,31870206)the Metasequoia funding of the Nanjing Forestry University,China。
文摘Living gymnosperms comprise four major groups:cycads,Ginkgo,conifers,and gnetophytes.Relationships among/within these lineages have not been fully resolved.Next generation sequencing has made available a large number of sequences,including both plastomes and single-copy nuclear genes,for reconstruction of solid phylogenetic trees.Recent advances in gymnosperm phylogenomic studies have updated our knowledge of gymnosperm systematics.Here,we review major advances of gymnosperm phylogeny over the past 10 years and propose an updated classification of extant gymnosperms.This new classification includes three classes(Cycadopsida,Ginkgoopsida,and Pinopsida),five subclasses(Cycadidae,Ginkgoidae,Cupressidae,Pinidae,and Gnetidae),eight orders(Cycadales,Ginkgoales,Araucariales,Cupressales,Pinales,Ephedrales,Gnetales,and Welwitschiales),13 families,and 86 genera.We also described six new tribes including Acmopyleae Y.Yang,Austrocedreae Y.Yang,Chamaecyparideae Y.Yang,Microcachrydeae Y.Yang,Papuacedreae Y.Yang,and Prumnopityeae Y.Yang,and made 27 new combinations in the genus Sabina.
文摘Background: Hepatic angiosarcoma is a rare malignant vascular tumor presenting unique treatment challenges.The aim of the present study was to determine the treatment and prognosis of this entity.Data sources: A systematic literature search was conducted using PubMed, Embase and Chinese BiomedicalLiterature database, to identify articles published from January 1980 to July 2017. Search termswere “hepatic angiosarcoma” and “liver angiosarcoma”. Additional articles were retrieved through manualsearch of bibliographies of the relevant articles. Pooled individual data concerning the prognosis followingvarious therapeutic modalities were analyzed.
基金Supported by Natural Science Foundation of Shanghai,No.09ZR1424700 and 114119a4700Minhang District Natural Science Foundation of Shanghai,No. 2009MHZ085grants from Minhang District Public Health Bureau of Shanghai,No.2009MW28
文摘AIM:To observe the curative effect of galactosylated chitosan(GC)/5-fluorouracil(5-FU) nanoparticles in liver caner mice and its side effects.METHODS:The GC/5-FU nanoparticle is a nanomaterial made by coupling GC and 5-FU.The release experiment was performed in vitro.The orthotropic liver cancer mouse models were established and divided into control,GC,5-FU and GC/5-FU groups.Mice in the control and GC group received an intravenous injection of 200 L saline and GC,respectively.Mice in the 5-FU and GC/5-FU groups received 200 L(containing 0.371 mg 5-FU) 5-FU and GC/5-FU,respectively.The tumor weight and survival time were observed.The cell cycle and apoptosis in tumor tissues were monitored by flow cytometry.The expression of p53,Bax,Bcl-2,caspase-3 and poly adenosine 50-diphosphate-ribose polymerase 1(PARP-1) was detected by immunohistochemistry,reverse transcription-polymerase chain reaction and Western blot.The serum blood biochemical parameters and cytotoxic activity of natural killer(NK) cell and cytotoxicity T lymphocyte(CTL) were measured.RESULTS:The GC/5-FU nanoparticle is a sustained release system.The drug loading was 6.12% ± 1.36%,the encapsulation efficiency was 81.82% ± 5.32%,and the Zeta potential was 10.34 ± 1.43 mV.The tumor weight in the GC/5-FU group(0.4361 ± 0.1153 g vs 1.5801 ± 0.2821 g,P < 0.001) and the 5-FU(0.7932 ± 0.1283 g vs 1.5801 ± 0.2821 g,P < 0.001) was significantly lower than that in the control group;GC/5FU treatment can significantly lower the tumor weight(0.4361 ± 0.1153 g vs 0.7932 ± 0.1283 g,P < 0.001),and the longest median survival time was seen in the GC/5-FU group,compared with the control(12 d vs 30 d,P < 0.001),GC(13 d vs 30 d,P < 0.001) and 5-FU groups(17 d vs 30 d,P < 0.001).Flow cytometry revealed that compared with the control,GC/5FU caused a higher rate of G0-G1 arrest(52.79% ± 13.42% vs 23.92% ± 9.09%,P = 0.014) and apoptosis(2.55% ± 1.10% vs 11.13% ± 11.73%,P < 0.001) in hepatic cancer cells.Analysis of the apoptosis pathways showed that GC/5-FU upregulated the expression of p53 at both the protein and the mRNA levels,which in turn lowered the ratio of Bcl-2/Bax expression;this led to the release of cytochrome C into the cytosol from the mitochondria and the subsequent activation of caspase-3.Upregulation of caspase-3 expression decreased the PARP-1 at both the mRNA and the protein levels,which contributed to apoptosis.5-FU increased the levels of aspartate aminotransferase and alanine aminotransferase,and decreased the numbers of platelet,white blood cell and lymphocyte and cytotoxic activities of CTL and NK cells,however,there were no such side effects in the GC/5-FU group.CONCLUSION:GC/5-FU nanoparticles can significantly inhibit the growth of liver cancer in mice via the p53 apoptosis pathway,and relieve the side effects and immunosuppression of 5-FU.
基金Foundation items: This research was funded by the National Natural Science Foundation of China (NSFC 30870290, 31071891 and 31471968)ACKNOWLEDGEMENTS We extend our thanks to Dr. Robert W. MURPHY for his valuable comments on this manuscript. We thank Jin-Min CHEN (Yunnan University), Bao-Lin ZHANG (Kunming Institute of Zoology, CAS) and Li-Fang PENG (Nanjing Forestry University) for their help in data processing. We further thank Dian- Cheng YANG (Nanjing Forestry University) and Jun-Sheng CUI (Anhui Agricultural University) for sampling.
文摘Six main mitochondrial DNA (mtDNA) lineages have been described in minnow (Zacco platypus) samples obtained from northern, western and southern China Perdices et al. (2004) predicted that further sampling of other tributaries might discover more lineages of this species. In this study, we collected 26 Zacco platypus individuals in the Huangshan area of eastern China and determined the cytochrome b (cytb) sequence variations. Combined with reported data in GenBank, we identified ten matrilines (Zacco A-J) in a total of 169 samples, with relatively high molecular divergence found among them. The Huangshan population had the greatest genetic variation among all sampled regions and hosted six of the ten matrilines. Our results highlight the significance of the Huangshan area for the conservation of Zacco platypus.
基金supported by the Science Foundation of China University of Petroleum-Beijing at Karamay (No. KL01JB201700003)Xinjiang Uygur Autonomous Region Tianchi 100 Talent Plan
文摘With shale oil reservoir pressure depletion and recovery of hydrocarbons from formations, the fracture apertures and conductivity are subject to reduction due to the interaction between stress effects and proppants. Suppose most of the proppants were concentrated in dominant fractures rather than sparsely allocated in the fracture network, the fracture conductivity would be less influenced by the induced stress effect. However, the merit of uniformly distributed proppants in the fracture network is that it increases the contact area for the injection gas with the shale matrix. In this paper, we address the question whether we should exploit or confine the fracture complexity for CO2-EOR in shale oil reservoirs. Two proppant transport scenarios were simulated in this paper: Case 1-the proppant is uniformly distributed in the complex fracture system, propagating a partially propped or un-propped fracture network; Case 2-the proppant primarily settles in simple planar fractures. A series of sensitivity studies of the fracture conductivity were performed to investigate the conductivity requirements in order to more efficiently produce from the shale reservoirs. Our simulation results in this paper show the potential of CO2 huff-n-puff to improve oil recovery in shale oil reservoirs. Simulation results indicate that the ultra-low permeability shales require an interconnected fracture network to maximize shale oil recovery in a reasonable time period. The well productivity of a fracture network with a conductivity of 4 mD ft achieves a better performance than that of planar fractures with an infinite conductivity. However, when the conductivity of fracture networks is inadequate,the planar fracture treatment design maybe a favorable choice. The available literature provides limited information on the relationship between fracture treatment design and the applicability of CO2 huff-n-puff in very low permeability shale formations. Very limited field test or laboratory data are available on the investigation of conductivity requirements for cyclic CO2 injection in shale oil reservoirs. In the context of CO2 huff-n-puff EOR, the effect of fracture complexity on well productivity was examined by simulation approaches.
文摘The effects of postthermal treatment and irradiation time on the structure and thermal stability of TiO2/polyacrylate nanocomposites by a sol-gel process in reverse micelles and subsequent rapid photopolymerization were investigated, and the hybrid films were characterized by thermal gravimetry analysis (TGA), X-ray photoelectron spectrum (XPS), and atomic force microscopy (AFM). XPS data suggested that the prolongation of irradiation time and the postthermal treatment promoted titania formation, with the former affecting more remarkably. TGA data showed that TiO2-hybrid films could upgrade the decomposition onset temperature (Tonset) as well as the temperature at which there is a maximum mass loss rate (Tmax). AFM data demonstrated that the inorganic titania particles with a mean diameter of 25.26-28.84 nm were homogeneously distributed in the organic matrix.
文摘AIM: To investigate the simplicity, reliability, andsafety of the application of single-layer mucosa-tomucosa pancreaticojejunal anastomosis in pancreaticoduodenectomy. METHODS: A retrospective analysis was performed on the data of patients who received pancreaticoduodenectomy completed by the same surgical group between January 2011 and April 2014 in the General Hospital of the People's Liberation Army. In total, 51 cases received single-layer mucosa-to-mucosa pancreaticojejunal anastomosis and 51 cases received double-layer pancreaticojejunal anastomosis. The diagnoses of pancreatic fistula and clinically relevant pancreatic fistula after pancreaticoduodenectomy were judged strictly by the International Study Group on pancreatic fistula definition. The preoperative and intraoperative data of these two groups were compared. χ2 test and Fisher's exact test were used to analyze the incidences of pancreatic fistula, peritoneal catheterization, abdominal infection and overall complications between the single-layer anastomosis group and double-layer anastomosis group. Rank sum test were used to analyze the difference in operation time, pancreaticojejunal anastomosis time, postoperative hospitalization time, total hospitalization time and hospitalization expenses between the single-layer anastomosis group and double-layer anastomosis group.RESULTS: Patients with grade A pancreatic fistula accounted for 15.69%(8/51) vs 15.69%(8/51)(P = 1.0000), and patients with grades B and C pancreatic fistula accounted for 9.80%(5/51) vs 52.94%(27/51)(P = 0.0000) in the single-layer and double-layer anastomosis groups. Although there was no significant difference in the percentage of patients with grade A pancreatic fistula, there was a significant difference in the percentage of patients with grades B and C pancreatic fistula between the two groups. Theoperation time(220.059 ± 60.602 min vs 379.412 ± 90.761 min, P = 0.000), pancreaticojejunal anastomosis time(17.922 ± 5.145 min vs 31.333 ± 7.776 min, P = 0.000), postoperative hospitalization time(18.588 ± 5.285 d vs 26.373 ± 15.815 d, P = 0.003), total hospitalization time(25.627 ± 6.551 d vs 33.706 ± 15.899 d, P = 0.002), hospitalization expenses(116787.667 ± 31900.927 yuan vs 162788.608 ± 129732.500 yuan, P = 0.001), as well as the incidences of pancreatic fistula [13/51(25.49%) vs 35/51(68.63%), P = 0.0000], peritoneal catheterization [0/51(0%) vs 6/51(11.76%), P = 0.0354], abdominal infection [1/51(1.96%) vs 11/51(21.57%), P = 0.0021], and overall complications [21/51(41.18%) vs 37/51(72.55%), P = 0.0014] in the single-layer anastomosis group were all lower than those in the double-layer anastomosis group. CONCLUSION: Single-layer mucosa-to-mucosa pancreaticojejunal anastomosis appears to be a simple, reliable, and safe method. Use of this method could reduce the postoperative incidence of complications.
基金This work was supported by funding from the Ginger Genome Project of Chongqing University of Arts and Sciences(2018)the Natural Science Foundation of Chongqing(cstc2019jcyj-msxmX0300,cstc2019jcyj-msxmX0697,CQYC201903201,cstc2019jscx-dxwtBX0028)+4 种基金the Foundation for High-level Talents of Chongqing University of Arts and Science(2017RTZ21,P2018TZ05)the Scientific and Technological Research Program of Chongqing Municipal Education Commission(KJZD-K202001304,KJQN201801339,KJQN201801330,KJQN201801335)the Foundation of Hubei Rural Science and Technology(2020BBA037)the State Key Research and Development Program of Hubei(2020BBA037)the Foundation of Laiwu Experimental Station of the National Characteristic Vegetable Industry System.
文摘Ginger(Zingiber officinale),the type species of Zingiberaceae,is one of the most widespread medicinal plants and spices.Here,we report a high-quality,chromosome-scale reference genome of ginger‘Zhugen’,a traditionally cultivated ginger in Southwest China used as a fresh vegetable,assembled from PacBio long reads,Illumina short reads,and high-throughput chromosome conformation capture(Hi-C)reads.The ginger genome was phased into two haplotypes,haplotype 1(1.53 Gb with a contig N50 of 4.68 M)and haplotype 0(1.51 Gb with a contig N50 of 5.28 M).Homologous ginger chromosomes maintained excellent gene pair collinearity.In 17,226 pairs of allelic genes,11.9%exhibited differential expression between alleles.Based on the results of ginger genome sequencing,transcriptome analysis,and metabolomic analysis,we proposed a backbone biosynthetic pathway of gingerol analogs,which consists of 12 enzymatic gene families,PAL,C4H,4CL,CST,C3’H,C3OMT,CCOMT,CSE,PKS,AOR,DHN,and DHT.These analyses also identified the likely transcription factor networks that regulate the synthesis of gingerol analogs.Overall,this study serves as an excellent resource for further research on ginger biology and breeding,lays a foundation for a better understanding of ginger evolution,and presents an intact biosynthetic pathway for species-specific gingerol biosynthesis.