Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death.China has the largest population of patients with traumatic spinal cord injury.Previous studies of traumatic ...Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death.China has the largest population of patients with traumatic spinal cord injury.Previous studies of traumatic spinal cord injury in China have mostly been regional in scope;national-level studies have been rare.To the best of our knowledge,no national-level study of treatment status and economic burden has been performed.This retrospective study aimed to examine the epidemiological and clinical features,treatment status,and economic burden of traumatic spinal cord injury in China at the national level.We included 13,465 traumatic spinal cord injury patients who were injured between January 2013 and December 2018 and treated in 30 hospitals in 11 provinces/municipalities representing all geographical divisions of China.Patient epidemiological and clinical features,treatment status,and total and daily costs were recorded.Trends in the percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department and cost of care were assessed by annual percentage change using the Joinpoint Regression Program.The percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department did not significantly change overall(annual percentage change,-0.5%and 2.1%,respectively).A total of 10,053(74.7%)patients underwent surgery.Only 2.8%of patients who underwent surgery did so within 24 hours of injury.A total of 2005(14.9%)patients were treated with high-dose(≥500 mg)methylprednisolone sodium succinate/methylprednisolone(MPSS/MP);615(4.6%)received it within 8 hours.The total cost for acute traumatic spinal cord injury decreased over the study period(-4.7%),while daily cost did not significantly change(1.0%increase).Our findings indicate that public health initiatives should aim at improving hospitals’ability to complete early surgery within 24 hours,which is associated with improved sensorimotor recovery,increasing the awareness rate of clinical guidelines related to high-dose MPSS/MP to reduce the use of the treatment with insufficient evidence.展开更多
Endometriosis is a common chronic gynecological disease with endometrial cell implantation outside the uterus.Angiogenesis is a major pathophysiology in endometriosis.Our previous studies have demonstrated that the pr...Endometriosis is a common chronic gynecological disease with endometrial cell implantation outside the uterus.Angiogenesis is a major pathophysiology in endometriosis.Our previous studies have demonstrated that the prodrug of epigallocatechin gallate(ProEGCG)exhibits superior anti-endometriotic and anti-angiogenic effects compared to epigallocatechin gallate(EGCG).However,their direct binding targets and underlying mechanisms for the differential effects remain unknown.In this study,we demonstrated that oral ProEGCG can be effective in preventing and treating endometriosis.Additionally,1D and 2D Proteome Integral Solubility Alteration assay-based chemical proteomics identified metadherin(MTDH)and PX domain containing serine/threonine kinase-like(PXK)as novel binding targets of EGCG and ProEGCG,respectively.Computational simulation and BioLayer interferometry were used to confirm their binding affinity.Our results showed that MTDH-EGCG inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEGCG inhibited epidermal growth factor(EGF)-mediated angiogenesis via the EGF/hypoxia-inducible factor(HIF-1a)/vascular endothelial growth factor(VEGF)pathway.In vitro and in vivo knockdown assays and microvascular network imaging further confirmed the involvement of these signaling pathways.Moreover,our study demonstrated that ProEGCG has superior therapeutic effects than EGCG by targeting distinct signal transduction pathways and may act as a novel antiangiogenic therapy for endometriosis.展开更多
Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the domi...Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.展开更多
This paper presents an attempt at assimilating clear-sky FY-4A Advanced Geosynchronous Radiation Imager(AGRI)radiances from two water vapor channels for the prediction of three landfalling typhoon events over the West...This paper presents an attempt at assimilating clear-sky FY-4A Advanced Geosynchronous Radiation Imager(AGRI)radiances from two water vapor channels for the prediction of three landfalling typhoon events over the West Pacific Ocean using the 3DVar data assimilation(DA)method along with the WRF model.A channel-sensitive cloud detection scheme based on the particle filter(PF)algorithm is developed and examined against a cloud detection scheme using the multivariate and minimum residual(MMR)algorithm and another traditional cloud mask–dependent cloud detection scheme.Results show that both channel-sensitive cloud detection schemes are effective,while the PF scheme is able to reserve more pixels than the MMR scheme for the same channel.In general,the added value of AGRI radiances is confirmed when comparing with the control experiment without AGRI radiances.Moreover,it is found that the analysis fields of the PF experiment are mostly improved in terms of better depicting the typhoon,including the temperature,moisture,and dynamical conditions.The typhoon track forecast skill is improved with AGRI radiance DA,which could be explained by better simulating the upper trough.The impact of assimilating AGRI radiances on typhoon intensity forecasts is small.On the other hand,improved rainfall forecasts from AGRI DA experiments are found along with reduced errors for both the thermodynamic and moisture fields,albeit the improvements are limited.展开更多
Silicon-containing aryl acetylene resin(PSA)is a new type of high-temperature resistant resin with excellent oxidation resistance,whereas antioxidant reaction mechanism of PSA resin under ultra-high temperatures still...Silicon-containing aryl acetylene resin(PSA)is a new type of high-temperature resistant resin with excellent oxidation resistance,whereas antioxidant reaction mechanism of PSA resin under ultra-high temperatures still remains unclear.Herein,the oxidation behavior and mechanisms of PSA resin are systematically investigated combining kinetic analysis and Reax FF molecular dynamics(MD)simulations.Thermogravimetric analysis indicates that the oxidation process of PSA resin undergoes two main steps:oxidative mass gain and oxidative degradation.The distributed activation energy model(DAEM)is employed for describing oxidation processes and the best-fit one is obtained using genetic algorithms and differential evolution.DAEM model demonstrates that the oxidative weight gain stage is dominated by two virtual reactants and the oxidative degradation stage consists of three virtual reactants.Correspondingly,the observation of MD reaction pathways indicates that oxygen oxidation of unsaturated structures occurs in the initial stage,which results in the formation of PSA resin oxides.Furthermore,cracked pieces react with O_(2)to generate CO and other chemicals in the second step.The resin matrix's great antioxidation resilience is illustrated by the formation of SiO_(2).The analysis based on MD simulations exhibits an efficient computational proof with the experiments and DAEM methods.Based on the results,a two-stage reaction mechanism is proposed,which provides important theoretical support for the subsequent study of the oxidation behavior of silica-based resins.展开更多
Traditional expert-designed branching rules in branch-and-bound(B&B) are static, often failing to adapt to diverse and evolving problem instances. Crafting these rules is labor-intensive, and may not scale well wi...Traditional expert-designed branching rules in branch-and-bound(B&B) are static, often failing to adapt to diverse and evolving problem instances. Crafting these rules is labor-intensive, and may not scale well with complex problems.Given the frequent need to solve varied combinatorial optimization problems, leveraging statistical learning to auto-tune B&B algorithms for specific problem classes becomes attractive. This paper proposes a graph pointer network model to learn the branch rules. Graph features, global features and historical features are designated to represent the solver state. The graph neural network processes graph features, while the pointer mechanism assimilates the global and historical features to finally determine the variable on which to branch. The model is trained to imitate the expert strong branching rule by a tailored top-k Kullback-Leibler divergence loss function. Experiments on a series of benchmark problems demonstrate that the proposed approach significantly outperforms the widely used expert-designed branching rules. It also outperforms state-of-the-art machine-learning-based branch-and-bound methods in terms of solving speed and search tree size on all the test instances. In addition, the model can generalize to unseen instances and scale to larger instances.展开更多
Microwave reflectometry is a powerful diagnostic that can measure the density profile and localized turbulence with high spatial and temporal resolution and will be used in ITER,so understanding the influence of plasm...Microwave reflectometry is a powerful diagnostic that can measure the density profile and localized turbulence with high spatial and temporal resolution and will be used in ITER,so understanding the influence of plasma perturbations on the reflect signal is important.The characteristics of the reflect signal from profile reflectometry,the time-of-flight(TOF)signal associated with the MHD instabilities,are investigated in EAST.Using a 1D full-wave simulation code by the Finite-DifferenceTime-Domain(FDTD)method,it is well validated that the local density flattening could induce the discontinuity of the simulated TOF signal and an obvious change of reflect amplitude.Experimental TOF signals under different types of MHD instabilities(sawtooth,sawtooth precursors and tearing mode)are studied in detail and show agreement with the simulation.Two new improved algorithms for detecting and localizing the radial positions of the low-order rational surface,the cross-correlation and gradient threshold(CGT)method and the 2D convolutional neural network approach(CNN)are presented for the first time.It is concluded that TOF signal analysis from profile reflectometry can provide a straightforward and localized measurement of the plasma perturbation from the edge to the core simultaneously and may be a complement or correction to the q-profile control,which will be beneficial for the advanced tokamak operation.展开更多
Phosphogypsum has often been used as an effective and environmentally friendly binder for partial replacement of cement,improving the engineering properties of slurries with high water content.However,the influence of...Phosphogypsum has often been used as an effective and environmentally friendly binder for partial replacement of cement,improving the engineering properties of slurries with high water content.However,the influence of phosphogypsum on the physicomechnical properties of stabilized soil subjected to wettingedrying cycles is not well understood to date.In this study,the effect of phosphogypsum on the durability of stabilized soil was studied by conducting a series of laboratory experiments,illustrating the changes in mass loss,pH value and unconfined compressive strength(qu)with wettingdrying cycles.The test results showed that the presence of phosphogypsum significantly restrained the mass loss in the early stage(lower than the 4th cycle),which in turn led to a higher qu of stabilized soil than that without phosphogypsum.After the 4th cycle,a sudden increase in mass loss was observed for stabilized soil with phosphogypsum,resulting in a significant drop in qu to a value lower than those without phosphogypsum at the 6th cycle.In addition,the qu of stabilized soils correlated well with the measured soil pH irrespective of phosphogypsum content for all wettingedrying tests.According to the microstructure observation via scanning electron microscope(SEM)and X-ray diffraction(XRD)tests,the mechanisms relating the sudden loss of qu for the stabilized soils with phosphogypsum after the 4th wetting-drying cycle are summarized as follows:(i)the disappearance of ettringite weakening the cementation bonding effect,(ii)the generation of a larger extent of microcrack,and(iii)a lower pH value,in comparison with the stabilized soil without phosphogypsum.展开更多
BACKGROUND Increasing evidence has demonstrated that N6-methyladenosine(m6A)RNA modification plays an essential role in a wide range of pathological conditions.Impaired autophagy is a critical hallmark of acute pancre...BACKGROUND Increasing evidence has demonstrated that N6-methyladenosine(m6A)RNA modification plays an essential role in a wide range of pathological conditions.Impaired autophagy is a critical hallmark of acute pancreatitis(AP).AIM To explore the role of the m6A modification of ZKSCAN3 in the regulation of autophagy in AP.METHODS The AP mouse cell model was established by cerulein-treated mouse pancreatic acinar cells(MPC-83),and the results were confirmed by the levels of amylase and inflammatory factors.Autophagy activity was evaluated by specific identification of the autophagy-related microstructure and the expression of autophagy-related genes.ZKSCAN3 and ALKBH5 were knocked down to study the function in AP.A m6A RNA binding protein immunoprecipitation assay was used to study how the m6A modification of ZKSCAN3 mRNA is regulated by ALKBH.RESULTS The increased expression of amylase and inflammatory factors in the supernatant and the accumulation of autophagic vacuoles verified that the AP mouse cell model was established.The downregulation of LAMP2 and upregulation of LC3-II/I and SQSTM1 demonstrated that autophagy was impaired in AP.The expression of ZKSCAN3 was upregulated in AP.Inhibition of ZKSCAN3 increased the expression of LAMP2 and decreased the expression of the inflammatory factors,LC3-II/I and SQSTM1.Furthermore,ALKBH5 was upregulated in AP.Knockdown of ALKBH5 downregulated ZKSCAN3 expression and restored decreased autophagic flux in AP.Notably,the bioinformatic analysis revealed 23 potential m6A modification sites on ZKSCAN3 mRNA.The m6A modification of ZKSCAN3 mRNA was significantly decreased in AP.Knockdown of ALKBH5 increased the modification of ZKSCAN3 mRNA,which confirmed that ALKBH5 upregulated ZKSCAN3 expression in a m6A-dependent manner.CONCLUSION ALKBH5 inhibits autophagic flux through m6A demethylation of ZKSCAN3 mRNA in AP,thereby aggravating the severity of the disease.展开更多
Hyperuricemia is a metabolic disorder caused by abnormal purine metabolism,resulting in abnormally high serum uric acid.In this study,a novel Levilactobacillus brevis PDD-5 isolated from salty vegetables was verified ...Hyperuricemia is a metabolic disorder caused by abnormal purine metabolism,resulting in abnormally high serum uric acid.In this study,a novel Levilactobacillus brevis PDD-5 isolated from salty vegetables was verified with the function of alleviating hyperuricemia.The relevant effects of L.brevis PDD-5 in lowering uric acid were analyzed by in vitro and in vivo experiments.The results showed that the L.brevis PDD-5 has(68.86±15.46)%of inosine uptake capacity and(95.75±3.30)%of guanosine uptake capacity in vitro.Oral administration of L.brevis PDD-5 to hyperuricemia rats reduced uric acid,creatinine,and urea nitrogen in serum,as well as decreased inosine and guanosine levels in the intestinal contents of rats.Analysis of relevant markers in the kidney by ELISA kits revealed that L.brevis PDD-5 alleviated oxidative stress and inflammation.Moreover,the gene expression of uric acid transporter 1(URAT1)and glucose transporter 9(GLUT9)was down-regulated,and the gene expression of organic anion transporter 1(OAT1)was up-regulated after treatment with L.brevis PDD-5.Western blot analysis showed that L.brevis PDD-5 alleviated hyperuricemia-induced kidney injury through the NLRP3 pathway.The se findings suggest that L.brevis PDD-5 can lower uric acid,repair kidney damage,and also has the potential to prevent uric acid nephropathy.展开更多
Dear Editor,In this letter, a finite-time convergent analysis of continuous action iterated dilemma(CAID) is proposed. In traditional evolutionary game theory, the strategy of the player is binary(cooperation or defec...Dear Editor,In this letter, a finite-time convergent analysis of continuous action iterated dilemma(CAID) is proposed. In traditional evolutionary game theory, the strategy of the player is binary(cooperation or defection), which limits the number of strategies a player can choose from.展开更多
Visual near-infrared imaging equipment has broad applications in various fields such as venipuncture,facial injections,and safety verification due to its noncontact,compact,and portable design.Currently,most studies u...Visual near-infrared imaging equipment has broad applications in various fields such as venipuncture,facial injections,and safety verification due to its noncontact,compact,and portable design.Currently,most studies utilize near-infrared single-wavelength for image acquisition of veins.However,many substances in the skin,including water,protein,and melanin can create significant background noise,which hinders accurate detection.In this paper,we developed a dual-wavelength imaging system with phase-locked denoising technology to acquire vein image.The signals in the effective region are compared by using the absorption valley and peak of hemoglobin at 700nm and 940nm,respectively.The phase-locked denoising algorithm is applied to decrease the noise and interference of complex surroundings from the images.The imaging results of the vein are successfully extracted in complex noise environment.It is demonstrated that the denoising effect on hand veins imaging can be improved with 57.3%by using our dual-wavelength phase-locked denoising technology.Consequently,this work proposes a novel approach for venous imaging with dual-wavelengths and phase-locked denoising algorithm to extract venous imaging results in complex noisy environment better.展开更多
In this paper we present a new experimental observation using a conventional reflectometry technique,poloidal correlation reflectometry(PCR),in the Experimental Advanced Superconducting Tokamak(EAST).The turbulence sp...In this paper we present a new experimental observation using a conventional reflectometry technique,poloidal correlation reflectometry(PCR),in the Experimental Advanced Superconducting Tokamak(EAST).The turbulence spectrum detected by the PCR system exhibits an asymmetry and induced Doppler shift f_(D)during the internal kink mode(IKM)rotation phase.This Doppler shift f_(D)is the target measurement of Doppler reflectometry,but captured by conventional reflectometry.Results show that the Doppler shift f_(D)is modulated by the periodic changes in the effective angle between the probing wave and cutoff layer normal,but not by plasma turbulence.The fishbone mode and saturated long-lived mode are typical IKMs,and this modulation phenomenon is observed in both cases.Moreover,the value of the Doppler shift f_(D)is positively correlated with the amplitude of the IKM,even when the latter is small.However,the positive and negative frequency components of the Doppler shift f_(D)can be asymmetric,which is related to the plasma configuration.A simulated analysis is performed by ray tracing to verify these observations.These results establish a clear link between f_(D)and IKM rotation,and are helpful for studying the characteristics of IKM and related physical phenomena.展开更多
Building energy consumption and building carbon emissions both account for more than 20%of their total national values in China.Building employing phase change material(PCM)for passive temperature control shows a prom...Building energy consumption and building carbon emissions both account for more than 20%of their total national values in China.Building employing phase change material(PCM)for passive temperature control shows a promising prospect in meeting the comfort demand and reducing energy consumption simultaneously.However,there is a lack of more detailed research on the interaction between the location and thickness of PCM and indoor natural convection,as well as indoor temperature distribution.In this study,the numerical model of a passive temperature-controlled building integrating the developed PCM module is established with the help of ANSYS.In which,the actual weather condition of Beijing city is set as the boundary conditions and the indoor natural convection is simulated with the consideration of radiation model.The effects of PCM’s thickness and location on the internal temperature field are analyzed and discussed.The results show that the room could maintain within the human comfort temperature range with the longest ratio of 94.10%and the shortest ratio of 51.04%as integrating PCM.In comparison,the value is only 26.70%without PCM.The room’s maximum temperature fluctuation can also be improved;it could be lowered by 64.4%compared to the normal condition.When the quantity of PCM is sufficient,further increasing the PCM amount results in a temperature fluctuation reduction of less than 0.1°C and does not increase the comfort time.Placing PCM on the wall induces an apparent variation in indoor temperature along the vertical direction.Conversely,placing PCM on the roof can lead to a heat transfer rate difference of up to seven times.The optimal placement of PCM depends on the difference between the environmental and phase change temperatures.If the difference is positive,placing PCM on the roof is more effective;conversely,the opposite holds.According to the results over the entire cycle,PCM application on vertical walls yields better performance.The significant difference in natural convection caused by the same thickness of PCM but different application positions,coupled with the influence of air movement on the melting and solidification of PCM,further impacts indoor temperature fluctuations and comfort.This study can provide guidance for the application location and thickness of PCM,especially for scenarios where temperature regulation is required at a specific time.展开更多
Embedded system design is the core course of the telecommunication major in engineering universities,which combines software and hardware through embedded development boards.Aiming at the problems existing in traditio...Embedded system design is the core course of the telecommunication major in engineering universities,which combines software and hardware through embedded development boards.Aiming at the problems existing in traditional teaching,this paper proposes curriculum teaching reform based on the outcome-based education(OBE)concept,including determining course objectives,reforming teaching modes and methods,and improving the curriculum assessment and evaluation system.After two semesters of practice,this method not only enhances students’learning initiative but also improves teaching quality.展开更多
基金supported by the National Natural Science Foundation of China (No. 51975596)the Fundamental Research Funds for the Central Universities of Central South University, China (No. CX20220285)。
基金supported by the National Key Research and Development Project,No.2019YFA0112100(to SF).
文摘Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death.China has the largest population of patients with traumatic spinal cord injury.Previous studies of traumatic spinal cord injury in China have mostly been regional in scope;national-level studies have been rare.To the best of our knowledge,no national-level study of treatment status and economic burden has been performed.This retrospective study aimed to examine the epidemiological and clinical features,treatment status,and economic burden of traumatic spinal cord injury in China at the national level.We included 13,465 traumatic spinal cord injury patients who were injured between January 2013 and December 2018 and treated in 30 hospitals in 11 provinces/municipalities representing all geographical divisions of China.Patient epidemiological and clinical features,treatment status,and total and daily costs were recorded.Trends in the percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department and cost of care were assessed by annual percentage change using the Joinpoint Regression Program.The percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department did not significantly change overall(annual percentage change,-0.5%and 2.1%,respectively).A total of 10,053(74.7%)patients underwent surgery.Only 2.8%of patients who underwent surgery did so within 24 hours of injury.A total of 2005(14.9%)patients were treated with high-dose(≥500 mg)methylprednisolone sodium succinate/methylprednisolone(MPSS/MP);615(4.6%)received it within 8 hours.The total cost for acute traumatic spinal cord injury decreased over the study period(-4.7%),while daily cost did not significantly change(1.0%increase).Our findings indicate that public health initiatives should aim at improving hospitals’ability to complete early surgery within 24 hours,which is associated with improved sensorimotor recovery,increasing the awareness rate of clinical guidelines related to high-dose MPSS/MP to reduce the use of the treatment with insufficient evidence.
基金supported by the GRF RGC&CRF,Hong Kong(Grant Nos.:475012 and C5045-20 EF)HMRF,Hong Kong(Grant No.:03141386)+3 种基金ITF,Hong Kong(Grant No.:ITS/209/12)UGC Direct Grant 2011,2012,2021.032HKOG Trust Fund 2011,2014,2019the National Natural Science Foundation of China(Grant Nos.:81974225 and 82201823)。
文摘Endometriosis is a common chronic gynecological disease with endometrial cell implantation outside the uterus.Angiogenesis is a major pathophysiology in endometriosis.Our previous studies have demonstrated that the prodrug of epigallocatechin gallate(ProEGCG)exhibits superior anti-endometriotic and anti-angiogenic effects compared to epigallocatechin gallate(EGCG).However,their direct binding targets and underlying mechanisms for the differential effects remain unknown.In this study,we demonstrated that oral ProEGCG can be effective in preventing and treating endometriosis.Additionally,1D and 2D Proteome Integral Solubility Alteration assay-based chemical proteomics identified metadherin(MTDH)and PX domain containing serine/threonine kinase-like(PXK)as novel binding targets of EGCG and ProEGCG,respectively.Computational simulation and BioLayer interferometry were used to confirm their binding affinity.Our results showed that MTDH-EGCG inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEGCG inhibited epidermal growth factor(EGF)-mediated angiogenesis via the EGF/hypoxia-inducible factor(HIF-1a)/vascular endothelial growth factor(VEGF)pathway.In vitro and in vivo knockdown assays and microvascular network imaging further confirmed the involvement of these signaling pathways.Moreover,our study demonstrated that ProEGCG has superior therapeutic effects than EGCG by targeting distinct signal transduction pathways and may act as a novel antiangiogenic therapy for endometriosis.
基金supported by the National Key Research and Development Program of China (2022YFD1800604)the China Agricultural Research System (CARS-41)the Heilongjiang Touyan Innovation Team Program of China
文摘Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.
基金primarily supported by the Chinese National Natural Science Foundation of China(Grant No. G42192553)Open Fund of Fujian Key Laboratory ofSevere Weather and Key Laboratory of Straits Severe Weather(Grant No. 2023KFKT03)+6 种基金the Open Project Fund of China Meteorological Administration Basin Heavy Rainfall Key Laboratory(Grant No. 2023BHR-Y20)the Open Fund of the State Key Laboratory of Remote Sensing Science (Grant No. OFSLRSS202321)the Program of Shanghai Academic/Technology Research Leader(Grant No. 21XD1404500)the Shanghai Typhoon Research Foundation (Grant No. TFJJ202107)the Chinese National Natural Science Foundation of China (Grant No. G41805016)the National Meteorological Center Foundation (Grant No. FY-APP-2021.0207)the High Performance Computing Center of Nanjing University of Information Science&Technology for their support of this work
文摘This paper presents an attempt at assimilating clear-sky FY-4A Advanced Geosynchronous Radiation Imager(AGRI)radiances from two water vapor channels for the prediction of three landfalling typhoon events over the West Pacific Ocean using the 3DVar data assimilation(DA)method along with the WRF model.A channel-sensitive cloud detection scheme based on the particle filter(PF)algorithm is developed and examined against a cloud detection scheme using the multivariate and minimum residual(MMR)algorithm and another traditional cloud mask–dependent cloud detection scheme.Results show that both channel-sensitive cloud detection schemes are effective,while the PF scheme is able to reserve more pixels than the MMR scheme for the same channel.In general,the added value of AGRI radiances is confirmed when comparing with the control experiment without AGRI radiances.Moreover,it is found that the analysis fields of the PF experiment are mostly improved in terms of better depicting the typhoon,including the temperature,moisture,and dynamical conditions.The typhoon track forecast skill is improved with AGRI radiance DA,which could be explained by better simulating the upper trough.The impact of assimilating AGRI radiances on typhoon intensity forecasts is small.On the other hand,improved rainfall forecasts from AGRI DA experiments are found along with reduced errors for both the thermodynamic and moisture fields,albeit the improvements are limited.
基金financially supported by National Natural Science Foundation of China(22008073,22078100,21878091)Shanghai Sailing Program(20YF1410600)。
文摘Silicon-containing aryl acetylene resin(PSA)is a new type of high-temperature resistant resin with excellent oxidation resistance,whereas antioxidant reaction mechanism of PSA resin under ultra-high temperatures still remains unclear.Herein,the oxidation behavior and mechanisms of PSA resin are systematically investigated combining kinetic analysis and Reax FF molecular dynamics(MD)simulations.Thermogravimetric analysis indicates that the oxidation process of PSA resin undergoes two main steps:oxidative mass gain and oxidative degradation.The distributed activation energy model(DAEM)is employed for describing oxidation processes and the best-fit one is obtained using genetic algorithms and differential evolution.DAEM model demonstrates that the oxidative weight gain stage is dominated by two virtual reactants and the oxidative degradation stage consists of three virtual reactants.Correspondingly,the observation of MD reaction pathways indicates that oxygen oxidation of unsaturated structures occurs in the initial stage,which results in the formation of PSA resin oxides.Furthermore,cracked pieces react with O_(2)to generate CO and other chemicals in the second step.The resin matrix's great antioxidation resilience is illustrated by the formation of SiO_(2).The analysis based on MD simulations exhibits an efficient computational proof with the experiments and DAEM methods.Based on the results,a two-stage reaction mechanism is proposed,which provides important theoretical support for the subsequent study of the oxidation behavior of silica-based resins.
基金supported by the Open Project of Xiangjiang Laboratory (22XJ02003)Scientific Project of the National University of Defense Technology (NUDT)(ZK21-07, 23-ZZCX-JDZ-28)+1 种基金the National Science Fund for Outstanding Young Scholars (62122093)the National Natural Science Foundation of China (72071205)。
文摘Traditional expert-designed branching rules in branch-and-bound(B&B) are static, often failing to adapt to diverse and evolving problem instances. Crafting these rules is labor-intensive, and may not scale well with complex problems.Given the frequent need to solve varied combinatorial optimization problems, leveraging statistical learning to auto-tune B&B algorithms for specific problem classes becomes attractive. This paper proposes a graph pointer network model to learn the branch rules. Graph features, global features and historical features are designated to represent the solver state. The graph neural network processes graph features, while the pointer mechanism assimilates the global and historical features to finally determine the variable on which to branch. The model is trained to imitate the expert strong branching rule by a tailored top-k Kullback-Leibler divergence loss function. Experiments on a series of benchmark problems demonstrate that the proposed approach significantly outperforms the widely used expert-designed branching rules. It also outperforms state-of-the-art machine-learning-based branch-and-bound methods in terms of solving speed and search tree size on all the test instances. In addition, the model can generalize to unseen instances and scale to larger instances.
基金supported by the Open Fund of Magnetic Confinement Laboratory of Anhui Province(No.2023 AMF03005)the China Postdoctoral Science Foundation(No.2021M703256)+4 种基金the Director Funding of Hefei Institutes of Physical Science,Chinese Academy of Sciences(No.YZJJ2022QN16)the National Key R&D Program of China(Nos.2022YFE03050003,2019YFE03080200,2019Y FE03040002,and 2022YFE03070004)National Natural Science Foundation of China(Nos.12075284,12175277,12275315 and 12275311)the National Magnetic Confinement Fusion Science Program of China(No.2022YFE03040001)the Science Foundation of the Institute of Plasma Physics,Chinese Academy of Sciences(No.DSJJ-2021-08)。
文摘Microwave reflectometry is a powerful diagnostic that can measure the density profile and localized turbulence with high spatial and temporal resolution and will be used in ITER,so understanding the influence of plasma perturbations on the reflect signal is important.The characteristics of the reflect signal from profile reflectometry,the time-of-flight(TOF)signal associated with the MHD instabilities,are investigated in EAST.Using a 1D full-wave simulation code by the Finite-DifferenceTime-Domain(FDTD)method,it is well validated that the local density flattening could induce the discontinuity of the simulated TOF signal and an obvious change of reflect amplitude.Experimental TOF signals under different types of MHD instabilities(sawtooth,sawtooth precursors and tearing mode)are studied in detail and show agreement with the simulation.Two new improved algorithms for detecting and localizing the radial positions of the low-order rational surface,the cross-correlation and gradient threshold(CGT)method and the 2D convolutional neural network approach(CNN)are presented for the first time.It is concluded that TOF signal analysis from profile reflectometry can provide a straightforward and localized measurement of the plasma perturbation from the edge to the core simultaneously and may be a complement or correction to the q-profile control,which will be beneficial for the advanced tokamak operation.
基金This research was financially supported by the National Natural Science Foundation of China(Grant Nos.52178328 and 52178361)the Fundamental Research Funds for the Open-end Research Fund of State Key Laboratory for Geomechanics and Deep Underground Engineering(Grant No.SKLGDUEK2114).
文摘Phosphogypsum has often been used as an effective and environmentally friendly binder for partial replacement of cement,improving the engineering properties of slurries with high water content.However,the influence of phosphogypsum on the physicomechnical properties of stabilized soil subjected to wettingedrying cycles is not well understood to date.In this study,the effect of phosphogypsum on the durability of stabilized soil was studied by conducting a series of laboratory experiments,illustrating the changes in mass loss,pH value and unconfined compressive strength(qu)with wettingdrying cycles.The test results showed that the presence of phosphogypsum significantly restrained the mass loss in the early stage(lower than the 4th cycle),which in turn led to a higher qu of stabilized soil than that without phosphogypsum.After the 4th cycle,a sudden increase in mass loss was observed for stabilized soil with phosphogypsum,resulting in a significant drop in qu to a value lower than those without phosphogypsum at the 6th cycle.In addition,the qu of stabilized soils correlated well with the measured soil pH irrespective of phosphogypsum content for all wettingedrying tests.According to the microstructure observation via scanning electron microscope(SEM)and X-ray diffraction(XRD)tests,the mechanisms relating the sudden loss of qu for the stabilized soils with phosphogypsum after the 4th wetting-drying cycle are summarized as follows:(i)the disappearance of ettringite weakening the cementation bonding effect,(ii)the generation of a larger extent of microcrack,and(iii)a lower pH value,in comparison with the stabilized soil without phosphogypsum.
基金Supported by National Natural Science Foundation of China,No.81802450and Natural Science Foundation of Hunan Province,No.2020JJ4133 and No.2021JJ31135.
文摘BACKGROUND Increasing evidence has demonstrated that N6-methyladenosine(m6A)RNA modification plays an essential role in a wide range of pathological conditions.Impaired autophagy is a critical hallmark of acute pancreatitis(AP).AIM To explore the role of the m6A modification of ZKSCAN3 in the regulation of autophagy in AP.METHODS The AP mouse cell model was established by cerulein-treated mouse pancreatic acinar cells(MPC-83),and the results were confirmed by the levels of amylase and inflammatory factors.Autophagy activity was evaluated by specific identification of the autophagy-related microstructure and the expression of autophagy-related genes.ZKSCAN3 and ALKBH5 were knocked down to study the function in AP.A m6A RNA binding protein immunoprecipitation assay was used to study how the m6A modification of ZKSCAN3 mRNA is regulated by ALKBH.RESULTS The increased expression of amylase and inflammatory factors in the supernatant and the accumulation of autophagic vacuoles verified that the AP mouse cell model was established.The downregulation of LAMP2 and upregulation of LC3-II/I and SQSTM1 demonstrated that autophagy was impaired in AP.The expression of ZKSCAN3 was upregulated in AP.Inhibition of ZKSCAN3 increased the expression of LAMP2 and decreased the expression of the inflammatory factors,LC3-II/I and SQSTM1.Furthermore,ALKBH5 was upregulated in AP.Knockdown of ALKBH5 downregulated ZKSCAN3 expression and restored decreased autophagic flux in AP.Notably,the bioinformatic analysis revealed 23 potential m6A modification sites on ZKSCAN3 mRNA.The m6A modification of ZKSCAN3 mRNA was significantly decreased in AP.Knockdown of ALKBH5 increased the modification of ZKSCAN3 mRNA,which confirmed that ALKBH5 upregulated ZKSCAN3 expression in a m6A-dependent manner.CONCLUSION ALKBH5 inhibits autophagic flux through m6A demethylation of ZKSCAN3 mRNA in AP,thereby aggravating the severity of the disease.
基金the National Natural Science Foundation of China(31972048,32272339)the National Key R&D Program of China(2021YFD2100104)for financial support。
文摘Hyperuricemia is a metabolic disorder caused by abnormal purine metabolism,resulting in abnormally high serum uric acid.In this study,a novel Levilactobacillus brevis PDD-5 isolated from salty vegetables was verified with the function of alleviating hyperuricemia.The relevant effects of L.brevis PDD-5 in lowering uric acid were analyzed by in vitro and in vivo experiments.The results showed that the L.brevis PDD-5 has(68.86±15.46)%of inosine uptake capacity and(95.75±3.30)%of guanosine uptake capacity in vitro.Oral administration of L.brevis PDD-5 to hyperuricemia rats reduced uric acid,creatinine,and urea nitrogen in serum,as well as decreased inosine and guanosine levels in the intestinal contents of rats.Analysis of relevant markers in the kidney by ELISA kits revealed that L.brevis PDD-5 alleviated oxidative stress and inflammation.Moreover,the gene expression of uric acid transporter 1(URAT1)and glucose transporter 9(GLUT9)was down-regulated,and the gene expression of organic anion transporter 1(OAT1)was up-regulated after treatment with L.brevis PDD-5.Western blot analysis showed that L.brevis PDD-5 alleviated hyperuricemia-induced kidney injury through the NLRP3 pathway.The se findings suggest that L.brevis PDD-5 can lower uric acid,repair kidney damage,and also has the potential to prevent uric acid nephropathy.
基金supported in part by the National Science Fund for Distinguished Young Scholarship of China (62025602)the National Natural Science Foundation of China (11931915, U22B2036)+2 种基金Fok Ying-Tong Education Foundationm China (171105)Technological lmnovation Team of Shaanxi Province (2020TD013)the Tencent Foundation and XPLORER PRIZE。
文摘Dear Editor,In this letter, a finite-time convergent analysis of continuous action iterated dilemma(CAID) is proposed. In traditional evolutionary game theory, the strategy of the player is binary(cooperation or defection), which limits the number of strategies a player can choose from.
基金funded by National Key R&D Pro-gram of China(2021YFC2103300)National Key R&D Program of China(2021YFA0715500)+2 种基金National Natural Science Foundation of China(NSFC)(12227901)Strategic Priority Research Program(B)of the Chinese Academy of Sciences(XDB0580000)Chinese Academy of Sciences President's International Fellowship Initiative(2021PT0007).
文摘Visual near-infrared imaging equipment has broad applications in various fields such as venipuncture,facial injections,and safety verification due to its noncontact,compact,and portable design.Currently,most studies utilize near-infrared single-wavelength for image acquisition of veins.However,many substances in the skin,including water,protein,and melanin can create significant background noise,which hinders accurate detection.In this paper,we developed a dual-wavelength imaging system with phase-locked denoising technology to acquire vein image.The signals in the effective region are compared by using the absorption valley and peak of hemoglobin at 700nm and 940nm,respectively.The phase-locked denoising algorithm is applied to decrease the noise and interference of complex surroundings from the images.The imaging results of the vein are successfully extracted in complex noise environment.It is demonstrated that the denoising effect on hand veins imaging can be improved with 57.3%by using our dual-wavelength phase-locked denoising technology.Consequently,this work proposes a novel approach for venous imaging with dual-wavelengths and phase-locked denoising algorithm to extract venous imaging results in complex noisy environment better.
基金supported by the National Key R&D Program of China(Nos.2022YFE03050003,2022YFE03020004,2019YFE03080200 and 2022YFE03070004)National Natural Science Foundation of China(Nos.12275315,11875289,12175277 and 11975271)+3 种基金partly supported by the Youth Science and Technology Talents Support Program(2020)by Anhui Association for Science and Technology(No.RCTJ202009)the Science Foundation of Institute of Plasma Physics,Chinese Academy of Sciences(No.DSJJ2021-08)the China Postdoctoral Science Foundation(No.2021M703256)the Director Funding of Hefei Institutes of Physical Science,Chinese Academy of Sciences(No.YZJJ2022QN16)。
文摘In this paper we present a new experimental observation using a conventional reflectometry technique,poloidal correlation reflectometry(PCR),in the Experimental Advanced Superconducting Tokamak(EAST).The turbulence spectrum detected by the PCR system exhibits an asymmetry and induced Doppler shift f_(D)during the internal kink mode(IKM)rotation phase.This Doppler shift f_(D)is the target measurement of Doppler reflectometry,but captured by conventional reflectometry.Results show that the Doppler shift f_(D)is modulated by the periodic changes in the effective angle between the probing wave and cutoff layer normal,but not by plasma turbulence.The fishbone mode and saturated long-lived mode are typical IKMs,and this modulation phenomenon is observed in both cases.Moreover,the value of the Doppler shift f_(D)is positively correlated with the amplitude of the IKM,even when the latter is small.However,the positive and negative frequency components of the Doppler shift f_(D)can be asymmetric,which is related to the plasma configuration.A simulated analysis is performed by ray tracing to verify these observations.These results establish a clear link between f_(D)and IKM rotation,and are helpful for studying the characteristics of IKM and related physical phenomena.
基金supported by National Innovation Talent Promotion Program(G2022013028L).
文摘Building energy consumption and building carbon emissions both account for more than 20%of their total national values in China.Building employing phase change material(PCM)for passive temperature control shows a promising prospect in meeting the comfort demand and reducing energy consumption simultaneously.However,there is a lack of more detailed research on the interaction between the location and thickness of PCM and indoor natural convection,as well as indoor temperature distribution.In this study,the numerical model of a passive temperature-controlled building integrating the developed PCM module is established with the help of ANSYS.In which,the actual weather condition of Beijing city is set as the boundary conditions and the indoor natural convection is simulated with the consideration of radiation model.The effects of PCM’s thickness and location on the internal temperature field are analyzed and discussed.The results show that the room could maintain within the human comfort temperature range with the longest ratio of 94.10%and the shortest ratio of 51.04%as integrating PCM.In comparison,the value is only 26.70%without PCM.The room’s maximum temperature fluctuation can also be improved;it could be lowered by 64.4%compared to the normal condition.When the quantity of PCM is sufficient,further increasing the PCM amount results in a temperature fluctuation reduction of less than 0.1°C and does not increase the comfort time.Placing PCM on the wall induces an apparent variation in indoor temperature along the vertical direction.Conversely,placing PCM on the roof can lead to a heat transfer rate difference of up to seven times.The optimal placement of PCM depends on the difference between the environmental and phase change temperatures.If the difference is positive,placing PCM on the roof is more effective;conversely,the opposite holds.According to the results over the entire cycle,PCM application on vertical walls yields better performance.The significant difference in natural convection caused by the same thickness of PCM but different application positions,coupled with the influence of air movement on the melting and solidification of PCM,further impacts indoor temperature fluctuations and comfort.This study can provide guidance for the application location and thickness of PCM,especially for scenarios where temperature regulation is required at a specific time.
基金This paper is one of the phased achievements of the Education and Teaching Reform Project of Guangdong University of Petrochemical Engineering in 2022(71013413080)the Research and Practice Project of Teaching and Teaching Reform of University-Level Higher Vocational Education in 2023(JY202353).
文摘Embedded system design is the core course of the telecommunication major in engineering universities,which combines software and hardware through embedded development boards.Aiming at the problems existing in traditional teaching,this paper proposes curriculum teaching reform based on the outcome-based education(OBE)concept,including determining course objectives,reforming teaching modes and methods,and improving the curriculum assessment and evaluation system.After two semesters of practice,this method not only enhances students’learning initiative but also improves teaching quality.