In order to provide a scientific basis for the control of Aeromonas allosaccharophila from Procambarus clarkii , a dominant strain was isolated from moribund P. clarkii in some farm in Hubei Province, and designated X...In order to provide a scientific basis for the control of Aeromonas allosaccharophila from Procambarus clarkii , a dominant strain was isolated from moribund P. clarkii in some farm in Hubei Province, and designated X1. It was preliminarily identified to be A. allosaccharophila through physiological-biochemical test. Further sequence analysis showed that 16S rDNA of this bacterium shared 90% identity with 16S rDNA of A. allosaccharophila , suggesting that it is A. allosaccharophila . Drug sensitivity test showed that isolate X1 was sensitive to doxycycline, cefotaxime, norfloxacin and gentamicin. In animal regression test, the bacterium the same as that from naturally-diseased P. clarkii could be isolated, with the same disease symptoms as well.展开更多
基金Supported by Special Fund for Technological Innovation in Hubei Province(2018ABA103)National Natural Science Foundation of China(31402343)Innovation Project of Wuhan Academy of Agricultural Sciences(CX201810)
文摘In order to provide a scientific basis for the control of Aeromonas allosaccharophila from Procambarus clarkii , a dominant strain was isolated from moribund P. clarkii in some farm in Hubei Province, and designated X1. It was preliminarily identified to be A. allosaccharophila through physiological-biochemical test. Further sequence analysis showed that 16S rDNA of this bacterium shared 90% identity with 16S rDNA of A. allosaccharophila , suggesting that it is A. allosaccharophila . Drug sensitivity test showed that isolate X1 was sensitive to doxycycline, cefotaxime, norfloxacin and gentamicin. In animal regression test, the bacterium the same as that from naturally-diseased P. clarkii could be isolated, with the same disease symptoms as well.