Objective To identify new genes that correlate with prognosis of clear-cell renal cell carcinoma(ccRCC)via bioinformatics analysis.Methods The gene expression profiles of 62 ccRCC and 54 normal kidney tissues were ava...Objective To identify new genes that correlate with prognosis of clear-cell renal cell carcinoma(ccRCC)via bioinformatics analysis.Methods The gene expression profiles of 62 ccRCC and 54 normal kidney tissues were available from the Gene Expression Omnibus database:GSE12606,GSE36895 and GSE66272.The differentially expressed genes were screened with GEO2R and J Venn online tools.Functional annotation including Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)was applied to identify the possible function of the hub genes involved in prognosis of ccRCC.In protein protein interaction network(PPI network),the STRING online tool was used to visualize the network of the differentially expressed genes,and the core gene was selected by MCODE App in Cytoscape software.Finally,GEPIA Survival Plot was performed to assess genes associated with worse survival.Results We totally found 648 diflerentially expressed genes,including 222 up-regulated genes and 426 down-regulated genes.PPI network showed that in 28 up-regulated genes 7(CCNE2,CDK1,CDC6,CCNB2,BUB1,TTK and PTTG1)enriched in cell cycle and 4 genes(CCNE2,CDK1,CCNB2 and RRM2)enriched in p53 signaling pathway.GEPIA Survival Plot assay revealed that ccRCC patients carrying CDK1,CCNB2,RRM2t BUB1,and PTTG1 had a worse survival.GEPIA Box Plot showed that BUB1,CCNB2,PTTG1,and RRM2 were over expressed in the ccRCC tissues in contrast to the normal tissues(P<O.OS).Conclusion ccRCC patients with the four up-regulated differentially expressed genes including BUB1,CCNB2,PTTG1,and RRM2 might manifest a poor prognosis.展开更多
研究黄精(Polygonatumsibiricum,PS)对CCl_(4)诱导的大鼠肝损伤的保护作用及其抗氧化机制。采用一次性腹腔注射50%四氯化碳油溶液建立大鼠急性肝损伤模型,用黄精水提物(PSAE)灌胃治疗,水飞蓟素作为阳性对照药物,连续7天。PSAE显著降低...研究黄精(Polygonatumsibiricum,PS)对CCl_(4)诱导的大鼠肝损伤的保护作用及其抗氧化机制。采用一次性腹腔注射50%四氯化碳油溶液建立大鼠急性肝损伤模型,用黄精水提物(PSAE)灌胃治疗,水飞蓟素作为阳性对照药物,连续7天。PSAE显著降低了血清丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶(AST、ALT和ALP)的水平(P<0.05),增加了肝组织谷胱甘肽(GSH)和超氧化物歧化酶(T-SOD)的水平(P<0.05),降低了肝组织中丙二醛的活性(P<0.05),并显著降低了肝细胞中的活性氧水平(P<0.05)。在肝组织中,核因子类红细胞2相关因子2(Nrf2),NAD(P)H醌脱氢酶1(NQO-1),血红素加氧酶1(HO-1)mRNA的表达水平显著升高,p53 mRNA表达显著降低(P<0.05),Bcl-2(P<0.05)和Bcl-x L mRNA的表达升高,HO-1蛋白表达升高,Keap-1 mRNA表达无明显差异(P>0.05)。因此,黄精对CCl_(4)诱导的急性肝损伤有较好的保护作用,其机制与有效调节Nrf2-Keap1-ARE通路相关基因和蛋白质的表达、抑制p53途径介导的肝细胞凋亡有关。展开更多
An efficient and catalytic protocol for highly stereoselective construction ofβ-mannopyranosylation has been developed.Glycosylation of 2,6-lactone-bridged mannopyranosyl ortho-hexynylbenzoate with various acceptors ...An efficient and catalytic protocol for highly stereoselective construction ofβ-mannopyranosylation has been developed.Glycosylation of 2,6-lactone-bridged mannopyranosyl ortho-hexynylbenzoate with various acceptors proceeded smoothly in the presence of 5%Hg(Ⅱ)at room temperature,resulting in the correspondingβ-mannosides in high yield and exclusiveβ-stereoselectivity.展开更多
基金the National Technical system of Chinese Medicinal Materials Industry(No.CARS-21)the Key Natural Science Projects of West Anhui University in China(No.WXZR201932)+4 种基金the Training Program of Innovate and Entrepreneurship of National College students in China(No.201810376058,201810376061)Anhui Provincial Natural Science Foundation in China(No.2017A030311022)Anhui Provincial University Natural Science Project in China(No.KJ2018A0413,KJ2017a407)Anhui Provincial Quality of Undergraduate project in China(No.2018zygc075,2018jyxm1153,2018jyxm1155)the Teaching and Research Projects of West Anhui University in China(No.wxxy2018026).
文摘Objective To identify new genes that correlate with prognosis of clear-cell renal cell carcinoma(ccRCC)via bioinformatics analysis.Methods The gene expression profiles of 62 ccRCC and 54 normal kidney tissues were available from the Gene Expression Omnibus database:GSE12606,GSE36895 and GSE66272.The differentially expressed genes were screened with GEO2R and J Venn online tools.Functional annotation including Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)was applied to identify the possible function of the hub genes involved in prognosis of ccRCC.In protein protein interaction network(PPI network),the STRING online tool was used to visualize the network of the differentially expressed genes,and the core gene was selected by MCODE App in Cytoscape software.Finally,GEPIA Survival Plot was performed to assess genes associated with worse survival.Results We totally found 648 diflerentially expressed genes,including 222 up-regulated genes and 426 down-regulated genes.PPI network showed that in 28 up-regulated genes 7(CCNE2,CDK1,CDC6,CCNB2,BUB1,TTK and PTTG1)enriched in cell cycle and 4 genes(CCNE2,CDK1,CCNB2 and RRM2)enriched in p53 signaling pathway.GEPIA Survival Plot assay revealed that ccRCC patients carrying CDK1,CCNB2,RRM2t BUB1,and PTTG1 had a worse survival.GEPIA Box Plot showed that BUB1,CCNB2,PTTG1,and RRM2 were over expressed in the ccRCC tissues in contrast to the normal tissues(P<O.OS).Conclusion ccRCC patients with the four up-regulated differentially expressed genes including BUB1,CCNB2,PTTG1,and RRM2 might manifest a poor prognosis.
基金Natural Science Foundation of the Anhui Higher Education Institutions of China(Grant No.KJ2019A0166)the National Natural Science Foundation of China(Grant No.31772786)。
文摘研究黄精(Polygonatumsibiricum,PS)对CCl_(4)诱导的大鼠肝损伤的保护作用及其抗氧化机制。采用一次性腹腔注射50%四氯化碳油溶液建立大鼠急性肝损伤模型,用黄精水提物(PSAE)灌胃治疗,水飞蓟素作为阳性对照药物,连续7天。PSAE显著降低了血清丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶(AST、ALT和ALP)的水平(P<0.05),增加了肝组织谷胱甘肽(GSH)和超氧化物歧化酶(T-SOD)的水平(P<0.05),降低了肝组织中丙二醛的活性(P<0.05),并显著降低了肝细胞中的活性氧水平(P<0.05)。在肝组织中,核因子类红细胞2相关因子2(Nrf2),NAD(P)H醌脱氢酶1(NQO-1),血红素加氧酶1(HO-1)mRNA的表达水平显著升高,p53 mRNA表达显著降低(P<0.05),Bcl-2(P<0.05)和Bcl-x L mRNA的表达升高,HO-1蛋白表达升高,Keap-1 mRNA表达无明显差异(P>0.05)。因此,黄精对CCl_(4)诱导的急性肝损伤有较好的保护作用,其机制与有效调节Nrf2-Keap1-ARE通路相关基因和蛋白质的表达、抑制p53途径介导的肝细胞凋亡有关。
基金supported by National Natural Science Foundation of China(Nos.21472119,22107061)the Science and Technology Program of Shaanxi Province,China(No.2018ZDXM-GY-152)。
文摘An efficient and catalytic protocol for highly stereoselective construction ofβ-mannopyranosylation has been developed.Glycosylation of 2,6-lactone-bridged mannopyranosyl ortho-hexynylbenzoate with various acceptors proceeded smoothly in the presence of 5%Hg(Ⅱ)at room temperature,resulting in the correspondingβ-mannosides in high yield and exclusiveβ-stereoselectivity.
