On the basis of single-factor experiment, an orthogonal experiment was carried out with culture temperature, initial pH, inoculation amount and liquid loading volume as factors at three levels. With the mycelium bioma...On the basis of single-factor experiment, an orthogonal experiment was carried out with culture temperature, initial pH, inoculation amount and liquid loading volume as factors at three levels. With the mycelium biomass as an index, the optimal secondary liquid seed culture conditions were obtained： culture temperature 27 ℃, initial pH 6.5, inoculation amount 13%, liquid loading volume 100 ml. Under the optimized conditions, mycelium biomass reached the maximum.展开更多
A wide range of morphological and physiological traits have changed between cultivated rice Oryza sativa and wild rice Oryza rufipogon under domestication. Here, we report cloning of the An-2 gene, encoding the Lonely...A wide range of morphological and physiological traits have changed between cultivated rice Oryza sativa and wild rice Oryza rufipogon under domestication. Here, we report cloning of the An-2 gene, encoding the Lonely Guy Like protein 6 （OsLOGL6）, which catalyzes the final step of cytokinin synthesis in O. rufipogon. The near-isogenic line harboring a wild allele of An-2 in the genetic background of the awnless indica Guangluai 4 shows that An-2 promotes awn elongation by enhancing cell division, but decreases grain production by reducing grains per panicle and tillers per plant. We reveal that a genetic variation in the An-2 locus has a large impact on reducing awn length and increasing tiller and grain numbers in domesticated rice. Analysis of gene expression patterns suggests that An-1 reg- ulates the formation of awn primordial, and An-2 promotes awn elongation. Nucleotide diversity of the An-2 locus in cultivated rice was found to be significantly reduced compared with that of wild rice, suggesting that the An-2 locus was subjected to artificial selection. We therefore propose that the selection of genetic variation in An-2 was due to reduced awn length and increased grain yield in cultivated rice.展开更多
基金Supported by Science and Technology Project of Huanggang City"Study on High-yield Ecological Cultivation Techniques of Agaricus bisporus"(2013C1022134)
文摘On the basis of single-factor experiment, an orthogonal experiment was carried out with culture temperature, initial pH, inoculation amount and liquid loading volume as factors at three levels. With the mycelium biomass as an index, the optimal secondary liquid seed culture conditions were obtained： culture temperature 27 ℃, initial pH 6.5, inoculation amount 13%, liquid loading volume 100 ml. Under the optimized conditions, mycelium biomass reached the maximum.
基金This work was supported by the Ministry of Science and Technology of China （2013CBA01404 and 2012AA10A302）, the Chinese Academy of Sciences （XDA08020101）, the Ministry of Agriculture of China （2014ZX08001004-002-010）, the National Natural Science Foundation of China （31121063 and 31471159）, and the Science and Technology Commission of Shanghai Municipality （13QA1403900）.We thank Prof. Fangqing Guo and Dr. Xiaoling Shang for providing some cytokinin substances and Prof. Kang Chong for providing the vector pTCK 303. We thank Dr. Yining Liu for the LC/MS experiments, Jiqin Li and Xiaoyan Gao for the SEM experiments, and Xiaoshu Gao for the confocal microscopy experiments. No conflict of interest declared.
文摘A wide range of morphological and physiological traits have changed between cultivated rice Oryza sativa and wild rice Oryza rufipogon under domestication. Here, we report cloning of the An-2 gene, encoding the Lonely Guy Like protein 6 （OsLOGL6）, which catalyzes the final step of cytokinin synthesis in O. rufipogon. The near-isogenic line harboring a wild allele of An-2 in the genetic background of the awnless indica Guangluai 4 shows that An-2 promotes awn elongation by enhancing cell division, but decreases grain production by reducing grains per panicle and tillers per plant. We reveal that a genetic variation in the An-2 locus has a large impact on reducing awn length and increasing tiller and grain numbers in domesticated rice. Analysis of gene expression patterns suggests that An-1 reg- ulates the formation of awn primordial, and An-2 promotes awn elongation. Nucleotide diversity of the An-2 locus in cultivated rice was found to be significantly reduced compared with that of wild rice, suggesting that the An-2 locus was subjected to artificial selection. We therefore propose that the selection of genetic variation in An-2 was due to reduced awn length and increased grain yield in cultivated rice.
