During intraoperative fluorescence navigation to remove various neoplasms and during pharmacokinetic studies of photosensitizers in laboratory animals,in many cases,the ratio of photosensitizer accumulation in the tum...During intraoperative fluorescence navigation to remove various neoplasms and during pharmacokinetic studies of photosensitizers in laboratory animals,in many cases,the ratio of photosensitizer accumulation in the tumor and normal tissue can reach>10-fold,which inevitably changes their optical properties.At the same time,the tumor formation process causes various metabolic and structural changes at cellular and tissue levels,which lead to changes in optical properties.A hardwaresoftware complex for the spectral-fluorescence studies of the content of fluorochromes in biological tissues with significantly different optical properties was developed,and it was tested on optical phantoms with various concentrations of photosensitizers,absorbers,and scatterers.To correct the influence of optical properties on the photosensitizer concentration analysis by fluorescence spectroscopy,we propose the spectrum-processing algorithm,which combines empirical and theory-based approaches.展开更多
The brain has an exceptionally high requirement for energy metabolism,with glucose serving as the exclusive energy source.Cancers,including glioblastoma,have a high glucose uptake and rely on aerobic glycolysis for en...The brain has an exceptionally high requirement for energy metabolism,with glucose serving as the exclusive energy source.Cancers,including glioblastoma,have a high glucose uptake and rely on aerobic glycolysis for energy metabolism.The alternation of high-efficiency oxidative phosphorylation to a low-efficiency aerobic glycolysis pathway(Warburg effect)provides macromolecules for biosynthesis and proliferation.Current research indicates that the specific metabolism in the tumor tissue and nonnal brain tissue in the glioma allows the use of 5-aminolevulinic acid(5 ALA)-induced protoporphyrin IX(PpIX)and methylene blue(MB)to monitor and correct the development of the tumor.The focus is on the detection of the differences between tumor cells and tumorassociated macrophages/microglia using spectroscopic and microscopic methods,based on the fluorescent signals and the difference in the drug accumulation of photosensitizers(PSs).Since 5 ALA has long been used effectively in the clinic for fluorescent surgical navigation,it was employed as an agent to identify the localization of tumor tissue and study its composition,particularly tumor and immune cells(macrophages),which have also been shown to actively accumulate PpIX.However,since PpIX is photodynamically active,it can be considered effective as the main target of tumor tissue for further successful photodynamic therapy.MB was employed to visualize resident microglia,which is important for their activation/deactivation to prevent the reprogramming of the immune cells by the tumor.Thus,using two drugs,it is possible to prevent crosstalk between tumor cells and the immune cells of different geneses.展开更多
基金The reported study was funded by Russian Foundation for Basic Research(RFBR)according to the research project No.18-29-01062.
文摘During intraoperative fluorescence navigation to remove various neoplasms and during pharmacokinetic studies of photosensitizers in laboratory animals,in many cases,the ratio of photosensitizer accumulation in the tumor and normal tissue can reach>10-fold,which inevitably changes their optical properties.At the same time,the tumor formation process causes various metabolic and structural changes at cellular and tissue levels,which lead to changes in optical properties.A hardwaresoftware complex for the spectral-fluorescence studies of the content of fluorochromes in biological tissues with significantly different optical properties was developed,and it was tested on optical phantoms with various concentrations of photosensitizers,absorbers,and scatterers.To correct the influence of optical properties on the photosensitizer concentration analysis by fluorescence spectroscopy,we propose the spectrum-processing algorithm,which combines empirical and theory-based approaches.
基金The reported study was funded by Russian Foundation for Basic Research according to the research project No.18-29-01062.
文摘The brain has an exceptionally high requirement for energy metabolism,with glucose serving as the exclusive energy source.Cancers,including glioblastoma,have a high glucose uptake and rely on aerobic glycolysis for energy metabolism.The alternation of high-efficiency oxidative phosphorylation to a low-efficiency aerobic glycolysis pathway(Warburg effect)provides macromolecules for biosynthesis and proliferation.Current research indicates that the specific metabolism in the tumor tissue and nonnal brain tissue in the glioma allows the use of 5-aminolevulinic acid(5 ALA)-induced protoporphyrin IX(PpIX)and methylene blue(MB)to monitor and correct the development of the tumor.The focus is on the detection of the differences between tumor cells and tumorassociated macrophages/microglia using spectroscopic and microscopic methods,based on the fluorescent signals and the difference in the drug accumulation of photosensitizers(PSs).Since 5 ALA has long been used effectively in the clinic for fluorescent surgical navigation,it was employed as an agent to identify the localization of tumor tissue and study its composition,particularly tumor and immune cells(macrophages),which have also been shown to actively accumulate PpIX.However,since PpIX is photodynamically active,it can be considered effective as the main target of tumor tissue for further successful photodynamic therapy.MB was employed to visualize resident microglia,which is important for their activation/deactivation to prevent the reprogramming of the immune cells by the tumor.Thus,using two drugs,it is possible to prevent crosstalk between tumor cells and the immune cells of different geneses.
