Nanomaterials with unique characteristics exhibit favorable therapeutic and diagnostic properties,implying their enormous potential as biomedical candidates. C60 has been used in gene- and drug-delivery, as imaging ag...Nanomaterials with unique characteristics exhibit favorable therapeutic and diagnostic properties,implying their enormous potential as biomedical candidates. C60 has been used in gene- and drug-delivery, as imaging agents, and as photosensitizers in cancer therapy. In this study, the influences of a cationic functionalized fullerene on cellular behavior of human colorectal cancer cell line(HT-29) were investigated. Results indicated that HT-29 treated with the studied compound showed a lower sensitivity but a significant impairment in migration and invasion by interfering with the activities of matrix metalloproteinases(MMP-2 and9). The presence of fullerene also altered the capacity of adhesion-related proteins to perform their activity,thereby inducing dramatically adverse effects on the cell physiological functions such as cell adhesion. Thus,our study suggests that this compound is a new potential anti-metastatic effector and a therapeutic component for malignant colorectal cancer.展开更多
Carbon nanomaterials offer excellent prospects as therapeutic agents,and among them,graphene quantum dots(GQDs)have gained considerable interest thanks to their aqueous solubility and intrinsic fluorescence,which enab...Carbon nanomaterials offer excellent prospects as therapeutic agents,and among them,graphene quantum dots(GQDs)have gained considerable interest thanks to their aqueous solubility and intrinsic fluorescence,which enable their possible use in theranostic approaches,if their biocompatibility and favorable pharmacokinetic are confirmed.We prepared ultra-small GQDs using an alternative,reproducible,top-down synthesis starting from graphene oxide with a nearly 100%conversion.The materials were tested to assess their safety,demonstrating good biocompatibility and ability in passing the ultrafiltration barrier using an in vitro model.This leads to renal excretion without affecting the kidneys.Moreover,we studied the GQDs in vivo biodistribution confirming their efficient renal clearance,and we demonstrated that the internalization mechanism into podocytes is caveolae-mediated.Therefore,considering the reported characteristics,it appears possible to vehiculate compounds to kidneys by means of GQDs,overcoming problems related to lysosomal degradation.展开更多
基金funded within the research contracts Nanocancer Friuli Venezia Giulia, Fra-2011University of TriesteItalian Ministry of Education MIUR (FIRB RBAP11ETKA and PRIN 2010N3T9M4 001)
文摘Nanomaterials with unique characteristics exhibit favorable therapeutic and diagnostic properties,implying their enormous potential as biomedical candidates. C60 has been used in gene- and drug-delivery, as imaging agents, and as photosensitizers in cancer therapy. In this study, the influences of a cationic functionalized fullerene on cellular behavior of human colorectal cancer cell line(HT-29) were investigated. Results indicated that HT-29 treated with the studied compound showed a lower sensitivity but a significant impairment in migration and invasion by interfering with the activities of matrix metalloproteinases(MMP-2 and9). The presence of fullerene also altered the capacity of adhesion-related proteins to perform their activity,thereby inducing dramatically adverse effects on the cell physiological functions such as cell adhesion. Thus,our study suggests that this compound is a new potential anti-metastatic effector and a therapeutic component for malignant colorectal cancer.
基金J.M.G.-D.acknowledges Spanish Ministry of Science,Innovation and Universities for his Juan de la Cierva Incorporación research contract(No.IJCI-2016-27789)This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie Grant Agreement No.734834(INFUSION)and No.734381(CARBO-IMmap)from MIUR.ICN2 is supported by the Severo Ochoa program from Spanish MINECO(No.SEV-2017-0706).
文摘Carbon nanomaterials offer excellent prospects as therapeutic agents,and among them,graphene quantum dots(GQDs)have gained considerable interest thanks to their aqueous solubility and intrinsic fluorescence,which enable their possible use in theranostic approaches,if their biocompatibility and favorable pharmacokinetic are confirmed.We prepared ultra-small GQDs using an alternative,reproducible,top-down synthesis starting from graphene oxide with a nearly 100%conversion.The materials were tested to assess their safety,demonstrating good biocompatibility and ability in passing the ultrafiltration barrier using an in vitro model.This leads to renal excretion without affecting the kidneys.Moreover,we studied the GQDs in vivo biodistribution confirming their efficient renal clearance,and we demonstrated that the internalization mechanism into podocytes is caveolae-mediated.Therefore,considering the reported characteristics,it appears possible to vehiculate compounds to kidneys by means of GQDs,overcoming problems related to lysosomal degradation.
