Background: A deletion of 32 bp in the nucleotide sequence of CCR5 gene results in a defective CCR5 which confers protection from HIV infection in the homozygous state, while reducing the rate of disease progression t...Background: A deletion of 32 bp in the nucleotide sequence of CCR5 gene results in a defective CCR5 which confers protection from HIV infection in the homozygous state, while reducing the rate of disease progression to AIDS and death in the heterozygous state. The status of the CCR5Δ32 gene has not been reported in Nigeria. Aim: This study was aimed at analyzing single nucleotide polymorphism of CCR5 gene among the Ijaws resident in Yenagoa, Nigeria. Methods: 100 subjects (75 HIV negative and 25 HIV positive control) were recruited for this study. The CCR5 genes were amplified by 2 Stage PCR reaction using GeneAmp 9700 PCR system utilizing specific primers that would flank 32 bp deletion, followed by agarose gel electrophoresis, DNA sequencing of 20 subjects was done followed by phylogenetic and polymorphism analysis. Results: The results showed that 75 (100%) of the HIV negative subjects had 189 base pair in their CCR5 gene. Nucleotide of the 20 (100%) of the sequenced samples were conservatively same and no SNP was observed. Conclusion: This study documented no SNPs in CCR5 gene of the study population hence;the study population has no protection from HIV infection.展开更多
Hypertension (HTN) is a major health problem worldwide. It can lead to cardiovascular disease and functional disturbances including haematological parameters. Full blood count (FBC) parameters of a total of 60 consecu...Hypertension (HTN) is a major health problem worldwide. It can lead to cardiovascular disease and functional disturbances including haematological parameters. Full blood count (FBC) parameters of a total of 60 consecutively-recruited hypertensive subjects and 30 age-matched non-hypertensive controls were studied between June and September, 2018. Ethylene Diamine Tetra Acetic Acid (EDTA) anticoagulated blood and serum from subjects and control participants were analyzed for FBC. The total white cell count (WBC), packed cell volume (PCV) haemoglobin (Hb), neutrophils and eosinophils were significantly lower among hypertensive subjects compared to non-hypertensive controls (p 40-year-old compared to those <40 years of age (p = 0.02 and 0.01) respectively. The RBC count was significantly higher among female compared to male hypertensives (p = 0.05). The haemoglobin and monocyte count were significantly higher among male compared to female hypertensives (p = 0.02 and 0.04) respectively. The monocyte count was significantly higher among hypertensive subjects of Hausa ethnic group compared to Fulani (t = 2.79, p < 0.01). The findings from this study indicates that the WBC, PCV, Hb, neutrophils and eosinophils were significantly lower among hypertensive subjects compared to non-hypertensive controls. Stage of the disease, age, gender and ethnicity had a significant effect on the FBC parameters of the hypertensive patients. There is need to step up public enlightenment programme to educate hypertensive patients on the need for attending clinic regularly for effective follow up. It is recommended that facilities for the routine monitoring of FBC parameters among hypertensive in the area be provided.展开更多
文摘Background: A deletion of 32 bp in the nucleotide sequence of CCR5 gene results in a defective CCR5 which confers protection from HIV infection in the homozygous state, while reducing the rate of disease progression to AIDS and death in the heterozygous state. The status of the CCR5Δ32 gene has not been reported in Nigeria. Aim: This study was aimed at analyzing single nucleotide polymorphism of CCR5 gene among the Ijaws resident in Yenagoa, Nigeria. Methods: 100 subjects (75 HIV negative and 25 HIV positive control) were recruited for this study. The CCR5 genes were amplified by 2 Stage PCR reaction using GeneAmp 9700 PCR system utilizing specific primers that would flank 32 bp deletion, followed by agarose gel electrophoresis, DNA sequencing of 20 subjects was done followed by phylogenetic and polymorphism analysis. Results: The results showed that 75 (100%) of the HIV negative subjects had 189 base pair in their CCR5 gene. Nucleotide of the 20 (100%) of the sequenced samples were conservatively same and no SNP was observed. Conclusion: This study documented no SNPs in CCR5 gene of the study population hence;the study population has no protection from HIV infection.
文摘Hypertension (HTN) is a major health problem worldwide. It can lead to cardiovascular disease and functional disturbances including haematological parameters. Full blood count (FBC) parameters of a total of 60 consecutively-recruited hypertensive subjects and 30 age-matched non-hypertensive controls were studied between June and September, 2018. Ethylene Diamine Tetra Acetic Acid (EDTA) anticoagulated blood and serum from subjects and control participants were analyzed for FBC. The total white cell count (WBC), packed cell volume (PCV) haemoglobin (Hb), neutrophils and eosinophils were significantly lower among hypertensive subjects compared to non-hypertensive controls (p 40-year-old compared to those <40 years of age (p = 0.02 and 0.01) respectively. The RBC count was significantly higher among female compared to male hypertensives (p = 0.05). The haemoglobin and monocyte count were significantly higher among male compared to female hypertensives (p = 0.02 and 0.04) respectively. The monocyte count was significantly higher among hypertensive subjects of Hausa ethnic group compared to Fulani (t = 2.79, p < 0.01). The findings from this study indicates that the WBC, PCV, Hb, neutrophils and eosinophils were significantly lower among hypertensive subjects compared to non-hypertensive controls. Stage of the disease, age, gender and ethnicity had a significant effect on the FBC parameters of the hypertensive patients. There is need to step up public enlightenment programme to educate hypertensive patients on the need for attending clinic regularly for effective follow up. It is recommended that facilities for the routine monitoring of FBC parameters among hypertensive in the area be provided.
