Sugarcane shoots and leaves consist of 35.2% cellulose, 23.43% hemicellulose, 12.6% lignin and 6.59% ash on dry solid （DS） basis and have the potential to serve as low cost feedstocks for ethanol production. To impr...Sugarcane shoots and leaves consist of 35.2% cellulose, 23.43% hemicellulose, 12.6% lignin and 6.59% ash on dry solid （DS） basis and have the potential to serve as low cost feedstocks for ethanol production. To improve the enzymatic digestibility of these biomass and bioethanol production, three pretreatment methods had been investigated and compared, including： （1） 2% w/v NaOH solution autoclaving pretreatment; （2） 2% w/v H2SO4 solution autoclaving pretreatment and （3） two steps of 2% w/v NaOH solution autoclaving followed by 2% w/v H2SO4 solution autoclaving pretreatment. Among them, the best result for ethanol production was obtained when 15 g DS of sugarcane shoots and leaves was pretreated by using two step of 2% w/v NaOH solution autoclaving followed by 2% w/v H2SO4 solution autoclaving. The highest ethanol concentration 30.40 g/L （92.65% in fermentation efficiency） was obtained from reducing sugar 89.25 g/L at 48 h. Moreover, the washing step of solid residue after pretreatment could reduce furfural and hydroxymethylfurfural （HMF） in all pretreatment methods when compared to unwashing solid residue after pretreatment.展开更多
文摘Sugarcane shoots and leaves consist of 35.2% cellulose, 23.43% hemicellulose, 12.6% lignin and 6.59% ash on dry solid （DS） basis and have the potential to serve as low cost feedstocks for ethanol production. To improve the enzymatic digestibility of these biomass and bioethanol production, three pretreatment methods had been investigated and compared, including： （1） 2% w/v NaOH solution autoclaving pretreatment; （2） 2% w/v H2SO4 solution autoclaving pretreatment and （3） two steps of 2% w/v NaOH solution autoclaving followed by 2% w/v H2SO4 solution autoclaving pretreatment. Among them, the best result for ethanol production was obtained when 15 g DS of sugarcane shoots and leaves was pretreated by using two step of 2% w/v NaOH solution autoclaving followed by 2% w/v H2SO4 solution autoclaving. The highest ethanol concentration 30.40 g/L （92.65% in fermentation efficiency） was obtained from reducing sugar 89.25 g/L at 48 h. Moreover, the washing step of solid residue after pretreatment could reduce furfural and hydroxymethylfurfural （HMF） in all pretreatment methods when compared to unwashing solid residue after pretreatment.
