Datura metel is one of psychoactive substances of great ethno-pharmacological significance often abused because of its unrestricted availability, yet, little is known about its mechanisms of action. This work was ther...Datura metel is one of psychoactive substances of great ethno-pharmacological significance often abused because of its unrestricted availability, yet, little is known about its mechanisms of action. This work was therefore aimed at assessing the activity of ethanolic seed extract of D. metel on Nissl substances, astrocytes, axonal and neuronal integrity of the medial prefrontal cortex (mPFC) of Wistar rats. Eighteen Wistar rats averaging 175 g were used and randomly assigned to three groups: group A rats (n = 6) were administered extract of 100 mg/kg bw, group B rats (n = 6) received extract of 200 mg/kg bw, and group C-control (n = 6) took distilled water only for 14 days. The results revealed that D. metel is deleterious to the health of Wistar rats at a dose-dependent rate as observed in its actions on the medial prefrontal cortex at 100 mg/kg bw and 200 mg/kg bw. The histological study of the treated Wistar rats exhibited features of disoriented neuronal integrity such as, chromatolysis, reduced protein synthesis due to loss of Nissl substances and nuclei, neuronal loss as well as axonal injuries.展开更多
Hippocampus plays a central role in the acquisition and recall of both episodic and spatial memory. Studies have shown that garlic has neuroprotective effects in various capacities and enhancement of different forms o...Hippocampus plays a central role in the acquisition and recall of both episodic and spatial memory. Studies have shown that garlic has neuroprotective effects in various capacities and enhancement of different forms of memory. However, the effect of garlic spatial memory and direct effect of garlic extract on the activities of membrane bound enzymes Na+/K+ ATPase and Ca2+ ATPase in the hippocampus of rat still remain elusive. Therefore, we studied the effect of ethanolic extract of garlic on spatial working memory using object location memory OLM test and the hippocampal Na+/K+ ATPase and Ca2+ ATPase activities. Sixteen male wistar rats weighted 120 - 150 g were used and divided into two groups of eight rats each. The control and experimental groups were treated 1ml of normal saline and 500 mg/kg body weight of ethanolic extract of garlic respectively orally for three weeks. OLM test was carried out on the two groups. Animals were sacrificed and the brains were removed, and hippocampi were carefully excised and homogenate was obtained. Homogenate was analyzed for Na+/K+ ATPase and Ca2+ ATPase activities. There was significant increase in the exploration time in experimental group when compared with control group (p +/K+ ATPase and Ca2+ ATPase activities in experimental group when compared with control group (p +/K+ ATPase and Ca2+ ATPase. Our findings provide potential mechanism and therapeutic target for memory deficit neurological disorders.展开更多
文摘Datura metel is one of psychoactive substances of great ethno-pharmacological significance often abused because of its unrestricted availability, yet, little is known about its mechanisms of action. This work was therefore aimed at assessing the activity of ethanolic seed extract of D. metel on Nissl substances, astrocytes, axonal and neuronal integrity of the medial prefrontal cortex (mPFC) of Wistar rats. Eighteen Wistar rats averaging 175 g were used and randomly assigned to three groups: group A rats (n = 6) were administered extract of 100 mg/kg bw, group B rats (n = 6) received extract of 200 mg/kg bw, and group C-control (n = 6) took distilled water only for 14 days. The results revealed that D. metel is deleterious to the health of Wistar rats at a dose-dependent rate as observed in its actions on the medial prefrontal cortex at 100 mg/kg bw and 200 mg/kg bw. The histological study of the treated Wistar rats exhibited features of disoriented neuronal integrity such as, chromatolysis, reduced protein synthesis due to loss of Nissl substances and nuclei, neuronal loss as well as axonal injuries.
文摘Hippocampus plays a central role in the acquisition and recall of both episodic and spatial memory. Studies have shown that garlic has neuroprotective effects in various capacities and enhancement of different forms of memory. However, the effect of garlic spatial memory and direct effect of garlic extract on the activities of membrane bound enzymes Na+/K+ ATPase and Ca2+ ATPase in the hippocampus of rat still remain elusive. Therefore, we studied the effect of ethanolic extract of garlic on spatial working memory using object location memory OLM test and the hippocampal Na+/K+ ATPase and Ca2+ ATPase activities. Sixteen male wistar rats weighted 120 - 150 g were used and divided into two groups of eight rats each. The control and experimental groups were treated 1ml of normal saline and 500 mg/kg body weight of ethanolic extract of garlic respectively orally for three weeks. OLM test was carried out on the two groups. Animals were sacrificed and the brains were removed, and hippocampi were carefully excised and homogenate was obtained. Homogenate was analyzed for Na+/K+ ATPase and Ca2+ ATPase activities. There was significant increase in the exploration time in experimental group when compared with control group (p +/K+ ATPase and Ca2+ ATPase activities in experimental group when compared with control group (p +/K+ ATPase and Ca2+ ATPase. Our findings provide potential mechanism and therapeutic target for memory deficit neurological disorders.
