Aims: The aim of the study was to assess the correlation between the number of CD34+ , CD117+ , c-met+ , CXCR4+ stem cells mobilized into peripheral blood, left ventricular ejection fraction(LVEF), NT-proBNP levels, a...Aims: The aim of the study was to assess the correlation between the number of CD34+ , CD117+ , c-met+ , CXCR4+ stem cells mobilized into peripheral blood, left ventricular ejection fraction(LVEF), NT-proBNP levels, and myocardial necrosis markers in patients with acute myocardial infarction(AMI). Methods and results: 43 patients with STEMI were enrolled. Stem cells number was measured using flow-cytometer and concentrations of NT-proBNP, SDF-1, G-CSF, VEGF, IL-6, and HGF were measured using ELISA kits. The number of stem cells mobilized early(<12 h) in AMI was significantly, positively correlated with LVEF: r=0.49(P=0.0012) for CD34+ cells, r=0.48(P=0.0018) for CXCR4+ cells, r=0.45(P=0.0043) for CD117+ cells, and r=0.41(P=0.01) for c-met+ cells and negatively correlated with NT-proBNP levels on admission r=- 0.35(P=0.024) for CD34+ cells, r=- 0.42(P=0.007) for CXCR4+ cells. In patients with LVEF ≤ 40% , the peak number of CD34+ , CXCR4+ , CD117+ , and c-met+ stem cells was significantly lower when compared patients with LVEF >40% . The number of CXCR4+ cells on admission and after 24 h was negatively correlated with respective cardiac Troponin I levels(r=- 0.37; P=0.029 and r=- 0.45, P=0.02) and maximum activity of CK-MB(r=- 0.37; P=0.021). No significant correlations between levels of haematopoietic cytokines and LVEF were found. Conclusion: The mobilization of CD34+ , CD117+ , CXCR4+ , c-met+ stem cells into peripheral blood early in STEMI is positively correlated with LVEF and negatively correlated with NT-proBNP levels and myocardial necrosis markers.展开更多
文摘Aims: The aim of the study was to assess the correlation between the number of CD34+ , CD117+ , c-met+ , CXCR4+ stem cells mobilized into peripheral blood, left ventricular ejection fraction(LVEF), NT-proBNP levels, and myocardial necrosis markers in patients with acute myocardial infarction(AMI). Methods and results: 43 patients with STEMI were enrolled. Stem cells number was measured using flow-cytometer and concentrations of NT-proBNP, SDF-1, G-CSF, VEGF, IL-6, and HGF were measured using ELISA kits. The number of stem cells mobilized early(<12 h) in AMI was significantly, positively correlated with LVEF: r=0.49(P=0.0012) for CD34+ cells, r=0.48(P=0.0018) for CXCR4+ cells, r=0.45(P=0.0043) for CD117+ cells, and r=0.41(P=0.01) for c-met+ cells and negatively correlated with NT-proBNP levels on admission r=- 0.35(P=0.024) for CD34+ cells, r=- 0.42(P=0.007) for CXCR4+ cells. In patients with LVEF ≤ 40% , the peak number of CD34+ , CXCR4+ , CD117+ , and c-met+ stem cells was significantly lower when compared patients with LVEF >40% . The number of CXCR4+ cells on admission and after 24 h was negatively correlated with respective cardiac Troponin I levels(r=- 0.37; P=0.029 and r=- 0.45, P=0.02) and maximum activity of CK-MB(r=- 0.37; P=0.021). No significant correlations between levels of haematopoietic cytokines and LVEF were found. Conclusion: The mobilization of CD34+ , CD117+ , CXCR4+ , c-met+ stem cells into peripheral blood early in STEMI is positively correlated with LVEF and negatively correlated with NT-proBNP levels and myocardial necrosis markers.
