Tannic acid (TA) and TA containing beverage have been proved to inhibit Ca2+-activated Cl- channel located apical membrane of the secretory cells. However, their effect on salivary fluid secretion is not well investig...Tannic acid (TA) and TA containing beverage have been proved to inhibit Ca2+-activated Cl- channel located apical membrane of the secretory cells. However, their effect on salivary fluid secretion is not well investigated. We used mouse ex Vivo submandibular gland perfusion technique to identify the general effect of TA and related beverage samples on muscarinic agonist carbachol induced fluid secretion. Green tea inhibited fluid secretion by 64% from the control, where oolong tea was by 53%, and red wine by 43% which was linked with their TA concentration. On the other hand, though TA was contained at 4.7 μM in white wine sample and 33 μM in coffee extract, no adverse effect was observed. In addition, coffee induced salivation in the absence of carbachol. TA had a negative effect on fluid secretion with a concentration dependent manner. The effects of TA on carbachol induced calcium increase showed identical as fluid secretion, which was initially no effect, and then gradually decreased over the time. These results demonstrate that TA directly inhibits the salivary fluid secretion and it affects not only Ca2+-activated Cl- channel but also intracellular Ca2+ increasing mechanisms.展开更多
The physiological and pharmacological responses of an ex vivo mouse submandibular gland were used to study fluid secretion and cell signaling in response to muscarinic stimulation at increasing temperatures. Saliva pr...The physiological and pharmacological responses of an ex vivo mouse submandibular gland were used to study fluid secretion and cell signaling in response to muscarinic stimulation at increasing temperatures. Saliva production at 37°C was 5.5-fold that at 25°C with pilocarpine stimulation and 9.8-fold that at 25°C with cevimeline stimulation. Both of these muscarinic agonists are used clinically. With the experimental agonist carbachol (CCh), saliva secretion was increased with an increase in temperature, but the CCh concentration producing the peak flow was the same in both dose-response curves, suggesting that the muscarinic receptor itself is not responsible for the temperature dependence. Purinergic agonists also induced temperature-dependent saliva production ex vivo. The calcium ionophore A23187 failed to have a significant effect on saliva production. The CCh-induced increase in intracellular Ca2+ also upregulated the initial increase and sustained the plateau phase of saliva flow. Thus, muscarinic receptor stimulation of saliva production is temperature sensitive due to an increase in intracellular Ca2+.展开更多
Purpose: In implant treatment, the abutments and superstructures form a transmucosal area. In addition to providing appropriate cleaning instructions to patients, its material properties must be carefully considered. ...Purpose: In implant treatment, the abutments and superstructures form a transmucosal area. In addition to providing appropriate cleaning instructions to patients, its material properties must be carefully considered. It is not clear how much plaque adhesion occurs at each site during treatment process, and the effect of materials on plaque adhesion. Methods: In this study, 33 patients, 13 maxillary cases and 20 mandibular cases, who had implants placed in the missing molars were evaluated for the plaque adhesion of provisional restorations made of acrylic resin and monolithic zirconia final restoration after a period of more than 4 weeks. In addition, oral hygiene instructions were thoroughly given for 12 cases at the time of staining after the provisional restorations, and re-evaluated in 4 weeks. Results: About 20% of the buccal-lingual area of the prosthetic device fabricated with acrylic resin showed plaque staining, whereas about 40% of the area of the mesial and distal. On the other hand, for the zirconia final restorations, the percentage of staining was about 5% for buccal and lingual surface and 10% for mesial and distal surface. The stained area ratio of the provisional restoration correlated with the zirconia superstructure area ratio. Even with careful oral hygiene, it became clear that the amount of plaque adhesion due to the difference in materials could not be overcome. Conclusion: The zirconia superstructure is extremely good from the viewpoint of plaque adhesion, but cases with a large stained area at the time of provisional restoration should be carefully followed up.展开更多
Purpose: Surgical templates produced by digital simulation and CAD/CAM allow for three-dimensional control of implant placement. However, due to clinical limitations, there are complications during the use of the temp...Purpose: Surgical templates produced by digital simulation and CAD/CAM allow for three-dimensional control of implant placement. However, due to clinical limitations, there are complications during the use of the template. The purpose of this study was to summarize the complications associated with the use of surgical templates for static computer-aided implant surgery. Methods: Complications were collected during the observation period, and then their implant sites were reanalyzed with simulation software. Results: There were 104 cases during the observation period, 5 cases had complications. Mechanical complications were observed in four cases, including three cases in which the frame of the template fractured during implant placement surgery and one case in which the sleeve fell off the surgical template. In one case, there was an error in the planned position. All cases were mandibular molar cases, and all cases of frame fracture were at the free end defect site. All cases had a Hounsfield unit of more than 700 at the implant site, and some of them had a significantly small jaw opening. Conclusion: Although the spread of CAD/CAM surgical templates has made it possible to avoid problems caused by the position of the implant, it has been difficult to avoid fractures in cases of mandibular free end defects with high Hounsfield unit.展开更多
文摘Tannic acid (TA) and TA containing beverage have been proved to inhibit Ca2+-activated Cl- channel located apical membrane of the secretory cells. However, their effect on salivary fluid secretion is not well investigated. We used mouse ex Vivo submandibular gland perfusion technique to identify the general effect of TA and related beverage samples on muscarinic agonist carbachol induced fluid secretion. Green tea inhibited fluid secretion by 64% from the control, where oolong tea was by 53%, and red wine by 43% which was linked with their TA concentration. On the other hand, though TA was contained at 4.7 μM in white wine sample and 33 μM in coffee extract, no adverse effect was observed. In addition, coffee induced salivation in the absence of carbachol. TA had a negative effect on fluid secretion with a concentration dependent manner. The effects of TA on carbachol induced calcium increase showed identical as fluid secretion, which was initially no effect, and then gradually decreased over the time. These results demonstrate that TA directly inhibits the salivary fluid secretion and it affects not only Ca2+-activated Cl- channel but also intracellular Ca2+ increasing mechanisms.
文摘The physiological and pharmacological responses of an ex vivo mouse submandibular gland were used to study fluid secretion and cell signaling in response to muscarinic stimulation at increasing temperatures. Saliva production at 37°C was 5.5-fold that at 25°C with pilocarpine stimulation and 9.8-fold that at 25°C with cevimeline stimulation. Both of these muscarinic agonists are used clinically. With the experimental agonist carbachol (CCh), saliva secretion was increased with an increase in temperature, but the CCh concentration producing the peak flow was the same in both dose-response curves, suggesting that the muscarinic receptor itself is not responsible for the temperature dependence. Purinergic agonists also induced temperature-dependent saliva production ex vivo. The calcium ionophore A23187 failed to have a significant effect on saliva production. The CCh-induced increase in intracellular Ca2+ also upregulated the initial increase and sustained the plateau phase of saliva flow. Thus, muscarinic receptor stimulation of saliva production is temperature sensitive due to an increase in intracellular Ca2+.
文摘Purpose: In implant treatment, the abutments and superstructures form a transmucosal area. In addition to providing appropriate cleaning instructions to patients, its material properties must be carefully considered. It is not clear how much plaque adhesion occurs at each site during treatment process, and the effect of materials on plaque adhesion. Methods: In this study, 33 patients, 13 maxillary cases and 20 mandibular cases, who had implants placed in the missing molars were evaluated for the plaque adhesion of provisional restorations made of acrylic resin and monolithic zirconia final restoration after a period of more than 4 weeks. In addition, oral hygiene instructions were thoroughly given for 12 cases at the time of staining after the provisional restorations, and re-evaluated in 4 weeks. Results: About 20% of the buccal-lingual area of the prosthetic device fabricated with acrylic resin showed plaque staining, whereas about 40% of the area of the mesial and distal. On the other hand, for the zirconia final restorations, the percentage of staining was about 5% for buccal and lingual surface and 10% for mesial and distal surface. The stained area ratio of the provisional restoration correlated with the zirconia superstructure area ratio. Even with careful oral hygiene, it became clear that the amount of plaque adhesion due to the difference in materials could not be overcome. Conclusion: The zirconia superstructure is extremely good from the viewpoint of plaque adhesion, but cases with a large stained area at the time of provisional restoration should be carefully followed up.
文摘Purpose: Surgical templates produced by digital simulation and CAD/CAM allow for three-dimensional control of implant placement. However, due to clinical limitations, there are complications during the use of the template. The purpose of this study was to summarize the complications associated with the use of surgical templates for static computer-aided implant surgery. Methods: Complications were collected during the observation period, and then their implant sites were reanalyzed with simulation software. Results: There were 104 cases during the observation period, 5 cases had complications. Mechanical complications were observed in four cases, including three cases in which the frame of the template fractured during implant placement surgery and one case in which the sleeve fell off the surgical template. In one case, there was an error in the planned position. All cases were mandibular molar cases, and all cases of frame fracture were at the free end defect site. All cases had a Hounsfield unit of more than 700 at the implant site, and some of them had a significantly small jaw opening. Conclusion: Although the spread of CAD/CAM surgical templates has made it possible to avoid problems caused by the position of the implant, it has been difficult to avoid fractures in cases of mandibular free end defects with high Hounsfield unit.
