Objective: To evaluate the antibacterial efficacy of five Indian medicinal plants such as Acalypha indica L.(A. indica), Aerva lanata(L.) Juss. ex Schult.(A. lanata), Clerodendrum inerme(L.) Gaertn., Pergularia daemia...Objective: To evaluate the antibacterial efficacy of five Indian medicinal plants such as Acalypha indica L.(A. indica), Aerva lanata(L.) Juss. ex Schult.(A. lanata), Clerodendrum inerme(L.) Gaertn., Pergularia daemia(Forsk.) Chiov. and Solanum surattense Burm. f. against opportunistic bacterial pathogens isolated from HIV infected patients for the potential phytoconstituents in plant extracts.Methods: The opportunistic bacterial pathogens such as Escherichia coli(E. coli),Pseudomonas aeruginosa, Salmonella typhi and Serratia marcescens from Gramnegative group and Staphylococcus aureus from Gram-positive group were isolated from HIV infected patients. The antibacterial efficacy of ethanolic extracts of selected medicinal plants was carried out by disc diffusion method. The potential phytoconstituents of medicinal plant extracts were identified by gas chromatography and mass spectrometry(GC–MS) analysis.Results: Among the five medicinal plants tested, A. indica and A. lanata showed the significant antibacterial activity. A. indica showed potential activity against Staphylococcus aureus and E. coli. A. lanata significantly exhibited antibacterial activity against E. coli, Salmonella typhi and Pseudomonas aeruginosa. A total of 19 phytoconstituents were identified in the ethanolic extract of A. indica and A. lanata by GC–MS analysis respectively.Conclusions: The results of the present investigation revealed that A. indica and A. lanata, possessed significant antibacterial activity when compared with the other plant extracts tested. The presence of 3-O-methyl-D-glucose by GC–MS analysis in both A. indica and A. lanata extracts has not been reported elsewhere in the literature and the findings in this study could be the first one to report.展开更多
Objective:To evaluate the antibacterial activity of zinc oxide nanoparticles synthesized using phycobilins of Anabaena variabilis NTSS17.Methods:The cyanobacterial isolate was collected from paddy field and morphologi...Objective:To evaluate the antibacterial activity of zinc oxide nanoparticles synthesized using phycobilins of Anabaena variabilis NTSS17.Methods:The cyanobacterial isolate was collected from paddy field and morphologically identified as Anabaena variabilis NTSS17,that produces a pigment i.e.phycobiliproteins.The biosynthesized zinc nanoparticles were characterized by different spectroscopic and analytical techniques such as UV-visible spectrophotometer,Fourier transform infrared spectroscopy and X-ray diffraction which confirmed the formation of zinc nanoparticles.Results:Antibacterial activity of zinc oxide nanoparticles was examined against Escherichia coli,Rhodococcus rhodochrous and Pseudomonas aeruginosa.The maximum zone of inhibition occurred at 5 mg/1000 mL concentration of zinc oxide nanoparticles.Conclusions:Due to potent antimicrobial and intrinsic properties of zinc oxide,it can be actively used for biomedical applications.展开更多
基金Supported by Department of Biotechnology Govt.of India(BT/PR4815/AAQ/3/587/2012)
文摘Objective: To evaluate the antibacterial efficacy of five Indian medicinal plants such as Acalypha indica L.(A. indica), Aerva lanata(L.) Juss. ex Schult.(A. lanata), Clerodendrum inerme(L.) Gaertn., Pergularia daemia(Forsk.) Chiov. and Solanum surattense Burm. f. against opportunistic bacterial pathogens isolated from HIV infected patients for the potential phytoconstituents in plant extracts.Methods: The opportunistic bacterial pathogens such as Escherichia coli(E. coli),Pseudomonas aeruginosa, Salmonella typhi and Serratia marcescens from Gramnegative group and Staphylococcus aureus from Gram-positive group were isolated from HIV infected patients. The antibacterial efficacy of ethanolic extracts of selected medicinal plants was carried out by disc diffusion method. The potential phytoconstituents of medicinal plant extracts were identified by gas chromatography and mass spectrometry(GC–MS) analysis.Results: Among the five medicinal plants tested, A. indica and A. lanata showed the significant antibacterial activity. A. indica showed potential activity against Staphylococcus aureus and E. coli. A. lanata significantly exhibited antibacterial activity against E. coli, Salmonella typhi and Pseudomonas aeruginosa. A total of 19 phytoconstituents were identified in the ethanolic extract of A. indica and A. lanata by GC–MS analysis respectively.Conclusions: The results of the present investigation revealed that A. indica and A. lanata, possessed significant antibacterial activity when compared with the other plant extracts tested. The presence of 3-O-methyl-D-glucose by GC–MS analysis in both A. indica and A. lanata extracts has not been reported elsewhere in the literature and the findings in this study could be the first one to report.
基金Supported by DST(Ref No.DST/IS-STAC/CO2-SR-163/13(G)).
文摘Objective:To evaluate the antibacterial activity of zinc oxide nanoparticles synthesized using phycobilins of Anabaena variabilis NTSS17.Methods:The cyanobacterial isolate was collected from paddy field and morphologically identified as Anabaena variabilis NTSS17,that produces a pigment i.e.phycobiliproteins.The biosynthesized zinc nanoparticles were characterized by different spectroscopic and analytical techniques such as UV-visible spectrophotometer,Fourier transform infrared spectroscopy and X-ray diffraction which confirmed the formation of zinc nanoparticles.Results:Antibacterial activity of zinc oxide nanoparticles was examined against Escherichia coli,Rhodococcus rhodochrous and Pseudomonas aeruginosa.The maximum zone of inhibition occurred at 5 mg/1000 mL concentration of zinc oxide nanoparticles.Conclusions:Due to potent antimicrobial and intrinsic properties of zinc oxide,it can be actively used for biomedical applications.