The present study was conducted to assess boar sperm susceptibility to oxidative stress generated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Semen was collected in replicates from three...The present study was conducted to assess boar sperm susceptibility to oxidative stress generated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Semen was collected in replicates from three experimental large white boars using the gloved-hand technique. Semen ejaculates from three boars were treated with different concentrations of H<sub>2</sub>O<sub>2</sub> for three hours. SYBR-14 and Propidium Iodide (PI) Live/ Dead assay kit was used to determine cell viability, and Yo-pro-1 and PI apoptosis kit was used to determine cell death, namely, apoptosis. Boar sperm motility obtained using computer aided sperm analysis (CASA) was between 90% and 100% with more than 98% viability with 0% apoptotic cells. In H<sub>2</sub>O<sub>2</sub> treated boar sperm cells, rapid (RAP) and progressive motility (PM) increased. Also, H<sub>2</sub>O<sub>2</sub> treatment induced a high positive correlation with apoptosis but high negative correlation with viability. Hydrogen peroxide decreased boar semen total motility (TM) by 10%. In addition, most of the boar sperm cells became apoptotic and lost 55% of viability under oxidative stress induced by H2O2. This study illustrated that boar semen was more susceptible to oxidative stress induced by H<sub>2</sub>O<sub>2</sub>.展开更多
文摘The present study was conducted to assess boar sperm susceptibility to oxidative stress generated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Semen was collected in replicates from three experimental large white boars using the gloved-hand technique. Semen ejaculates from three boars were treated with different concentrations of H<sub>2</sub>O<sub>2</sub> for three hours. SYBR-14 and Propidium Iodide (PI) Live/ Dead assay kit was used to determine cell viability, and Yo-pro-1 and PI apoptosis kit was used to determine cell death, namely, apoptosis. Boar sperm motility obtained using computer aided sperm analysis (CASA) was between 90% and 100% with more than 98% viability with 0% apoptotic cells. In H<sub>2</sub>O<sub>2</sub> treated boar sperm cells, rapid (RAP) and progressive motility (PM) increased. Also, H<sub>2</sub>O<sub>2</sub> treatment induced a high positive correlation with apoptosis but high negative correlation with viability. Hydrogen peroxide decreased boar semen total motility (TM) by 10%. In addition, most of the boar sperm cells became apoptotic and lost 55% of viability under oxidative stress induced by H2O2. This study illustrated that boar semen was more susceptible to oxidative stress induced by H<sub>2</sub>O<sub>2</sub>.
