Teicoplanin (TCP) is a multiple drug-resistant lipoglycopeptide antibiotic produced by fermenting Actinoplanes teichomyceticus. In this study, a mixture of TCP with the Tris-HCl buffer (TCP-Tris- HCl) was used to simu...Teicoplanin (TCP) is a multiple drug-resistant lipoglycopeptide antibiotic produced by fermenting Actinoplanes teichomyceticus. In this study, a mixture of TCP with the Tris-HCl buffer (TCP-Tris- HCl) was used to simulate TCP fermentation broth. The reagent-free, rapid and simultaneous quantitative analysis models for TCP and Tris in the TCP-Tris-HCl mixtures were established by near-infrared (NIR) spectroscopy. The equidistant combination partial least squares (EC-PLS) method and the equivalent model sets were proposed, the simplest equivalent model with the smallest number of wavelengths were further selected. The initial wavelength, number of wavelengths, number of wavelength gaps, number of PLS factors were 1520 nm, 28, 5, 5 for TCP and 1084 nm, 13, 6, 4 for Tris, respectively. Compared with the optimal EC-PLS models, the simplest equivalent models adopted fewer wavelengths. Thus, the redundant wavelengths were removed, the models were further simplified. The root-mean-square errors (SEP) and correlation coefficients (R<sub>P</sub>) for prediction were 0.043 mg·mL<sup>-1</sup> and 0.9998 for TCP, and 0.222 mg·mL<sup>-1</sup> and 0.9989 for Tris, respectively. The results indicate that NIR method can be applied to highly accurate quantitative analysis for TCP and provide valuable references for further application to TCP fermentation broth.展开更多
Teicoplanin(TCP)is an important lipoglycopeptide antibiotic produced by fermenting Acti-noplanes teichomyceticus.The change in TCP concentration is important to measure in the fermentation process.In this study,a reag...Teicoplanin(TCP)is an important lipoglycopeptide antibiotic produced by fermenting Acti-noplanes teichomyceticus.The change in TCP concentration is important to measure in the fermentation process.In this study,a reagent-free and rapid quantification method for TCP in the TCP-Tris-HCl mixture samples was developed using near infrared(NIR)spectroscopy by focusing our attention on the fermentation process for TCP.The absorbance optimization(AO)partial least squares(PLS)was proposed and integrated with the moving window(MW)PLS,which is called AO-MW-PLS method,to select appropriate wavebands.Amodel set that includes various wavebands that were equivalent to the optimal AO-MW-PLS waveband was,proposed based on statistical considerations.The public region of all equivalent wavebands was just one of the equivalent wavebands.The obtained public regions were 1540-1868 nm for TCP and 1114-1310 nm for Tris.The root-mean-square error and correlation coeficient for leave-one-out cross validation were 0.046 mg mL^(-1)and 0.9998 mg mL^(-1)for TCP,and 0.235 mg mL^(-1)and 0.9986 mg mL^(-1)for Tris,respectively.All the models achieved highly accurate prediction effects,and the selected wavebands provided valuable references for designing specialized spectrometers.This study provided a valuable reference for further application of the proposed methods to TCP fermentation broth and to other spectroscopic analysis fields.展开更多
文摘Teicoplanin (TCP) is a multiple drug-resistant lipoglycopeptide antibiotic produced by fermenting Actinoplanes teichomyceticus. In this study, a mixture of TCP with the Tris-HCl buffer (TCP-Tris- HCl) was used to simulate TCP fermentation broth. The reagent-free, rapid and simultaneous quantitative analysis models for TCP and Tris in the TCP-Tris-HCl mixtures were established by near-infrared (NIR) spectroscopy. The equidistant combination partial least squares (EC-PLS) method and the equivalent model sets were proposed, the simplest equivalent model with the smallest number of wavelengths were further selected. The initial wavelength, number of wavelengths, number of wavelength gaps, number of PLS factors were 1520 nm, 28, 5, 5 for TCP and 1084 nm, 13, 6, 4 for Tris, respectively. Compared with the optimal EC-PLS models, the simplest equivalent models adopted fewer wavelengths. Thus, the redundant wavelengths were removed, the models were further simplified. The root-mean-square errors (SEP) and correlation coefficients (R<sub>P</sub>) for prediction were 0.043 mg·mL<sup>-1</sup> and 0.9998 for TCP, and 0.222 mg·mL<sup>-1</sup> and 0.9989 for Tris, respectively. The results indicate that NIR method can be applied to highly accurate quantitative analysis for TCP and provide valuable references for further application to TCP fermentation broth.
基金the Science and Tech-nology Project of Guangdong Province of China,(Nos.2014A020213016 and 2014A020212445)the Science and Technology Project of Guangzhou of China(No.2011Y5-00002).
文摘Teicoplanin(TCP)is an important lipoglycopeptide antibiotic produced by fermenting Acti-noplanes teichomyceticus.The change in TCP concentration is important to measure in the fermentation process.In this study,a reagent-free and rapid quantification method for TCP in the TCP-Tris-HCl mixture samples was developed using near infrared(NIR)spectroscopy by focusing our attention on the fermentation process for TCP.The absorbance optimization(AO)partial least squares(PLS)was proposed and integrated with the moving window(MW)PLS,which is called AO-MW-PLS method,to select appropriate wavebands.Amodel set that includes various wavebands that were equivalent to the optimal AO-MW-PLS waveband was,proposed based on statistical considerations.The public region of all equivalent wavebands was just one of the equivalent wavebands.The obtained public regions were 1540-1868 nm for TCP and 1114-1310 nm for Tris.The root-mean-square error and correlation coeficient for leave-one-out cross validation were 0.046 mg mL^(-1)and 0.9998 mg mL^(-1)for TCP,and 0.235 mg mL^(-1)and 0.9986 mg mL^(-1)for Tris,respectively.All the models achieved highly accurate prediction effects,and the selected wavebands provided valuable references for designing specialized spectrometers.This study provided a valuable reference for further application of the proposed methods to TCP fermentation broth and to other spectroscopic analysis fields.
