Magnetic fluorescent dual-drug nanocomposites（MFDDs） were developed with the aim of simultaneouly delivering two different anticancer drugs, kaempferol（KAE） and paclitaxel（PTX）. Firstly, Fe3O4/bovine serum albu...Magnetic fluorescent dual-drug nanocomposites（MFDDs） were developed with the aim of simultaneouly delivering two different anticancer drugs, kaempferol（KAE） and paclitaxel（PTX）. Firstly, Fe3O4/bovine serum albumin（Fe3O4/BSA） composite microspheres with physically entrapped KAE were prepared, then microspheres were modified with PTX/graphene quantum dots（PTX/GQDs） through chemically bonding, and the MFDDs were obtained. The properties of nancomposites were characterized by X-ray diffractometry, Fourier-transform infrared spectroscopy, transmission electron microscopy, vibrating sample magnetometry and X-ray fluorescence spectrometry. It was found that the superparamagnetic nanocomposites had ultrafine size（below 110 nm）, high saturation magnetization of 24.36 emu/g, and significant fluorescence. Furthermore, the cumulative in vitro release of the MFDDs exhibited controlled drug release. Cell viability experiments confirmed that the co-administration of KAE with PTX had a superior cytotoxicity to the Hela cells compared with single drug-loaded forms. Therefore, dual anticancer drug-loaded MFDDs have the potential to be used for cancer combined chemotherapy.展开更多
Objective To investigate the value of P-selectin, thrombomodulin(TM)and von Willebrand factor(vWF)in identifying severe acute pancreatitis(SAP)at an early stage of the disease.Methods 80 patients with acute pancreatit...Objective To investigate the value of P-selectin, thrombomodulin(TM)and von Willebrand factor(vWF)in identifying severe acute pancreatitis(SAP)at an early stage of the disease.Methods 80 patients with acute pancreatitis(25 mild, 30 moderate and 25 severe)were selected in this study when they were admitted within 24 hours of the onset of symptoms. 20 healthy volunteers were collected as the healthy control. Enzyme-linked immunosorbent assay(ELISA)was used to determine the levels of vascular endothelial cell damage markers(P-selectin, TM and vWF)in SAP patients and the control group.Results The levels of 3 markers were significantly higher in SAP patients than in the mild and moderate acute pancreatitis patients and healthy volunteers(P<0.05). P-selectin, TM and vWF were independent markers for the development of SAP and had higher discriminative powers than conventional marker tumor necrosis factor(TNF-α)(P<0.05). They were similar to the APACHE-Ⅱ scoring system in distinguishing SAP at an early stage.Conclusions P-selectin, TM and vWF are independent markers to identify the development of SAP at an early stage. They are obviously superior to TNF-α and similar to the APACHE-Ⅱscoring system.展开更多
基金Funded by the Natural Science Fund of Jiangsu Overseas Research&Training Program for University Prominent Young&Middle-aged Teachers and Presidentsthe Natural Science Fund of Jiangsu Province(No.BK20130094)+1 种基金the Enterpriseuniversities Cooperative Innovation Fund of Jiangsu Province(No.BY2014016)the National Natural Science Foundation of China(No.51103066)
文摘Magnetic fluorescent dual-drug nanocomposites（MFDDs） were developed with the aim of simultaneouly delivering two different anticancer drugs, kaempferol（KAE） and paclitaxel（PTX）. Firstly, Fe3O4/bovine serum albumin（Fe3O4/BSA） composite microspheres with physically entrapped KAE were prepared, then microspheres were modified with PTX/graphene quantum dots（PTX/GQDs） through chemically bonding, and the MFDDs were obtained. The properties of nancomposites were characterized by X-ray diffractometry, Fourier-transform infrared spectroscopy, transmission electron microscopy, vibrating sample magnetometry and X-ray fluorescence spectrometry. It was found that the superparamagnetic nanocomposites had ultrafine size（below 110 nm）, high saturation magnetization of 24.36 emu/g, and significant fluorescence. Furthermore, the cumulative in vitro release of the MFDDs exhibited controlled drug release. Cell viability experiments confirmed that the co-administration of KAE with PTX had a superior cytotoxicity to the Hela cells compared with single drug-loaded forms. Therefore, dual anticancer drug-loaded MFDDs have the potential to be used for cancer combined chemotherapy.
文摘Objective To investigate the value of P-selectin, thrombomodulin(TM)and von Willebrand factor(vWF)in identifying severe acute pancreatitis(SAP)at an early stage of the disease.Methods 80 patients with acute pancreatitis(25 mild, 30 moderate and 25 severe)were selected in this study when they were admitted within 24 hours of the onset of symptoms. 20 healthy volunteers were collected as the healthy control. Enzyme-linked immunosorbent assay(ELISA)was used to determine the levels of vascular endothelial cell damage markers(P-selectin, TM and vWF)in SAP patients and the control group.Results The levels of 3 markers were significantly higher in SAP patients than in the mild and moderate acute pancreatitis patients and healthy volunteers(P<0.05). P-selectin, TM and vWF were independent markers for the development of SAP and had higher discriminative powers than conventional marker tumor necrosis factor(TNF-α)(P<0.05). They were similar to the APACHE-Ⅱ scoring system in distinguishing SAP at an early stage.Conclusions P-selectin, TM and vWF are independent markers to identify the development of SAP at an early stage. They are obviously superior to TNF-α and similar to the APACHE-Ⅱscoring system.
