When harvested the potato has a high moisture content, this could make its transportation and storage difficult. To help solve the transportation difficulties, potato is made into whole flour by many food processing c...When harvested the potato has a high moisture content, this could make its transportation and storage difficult. To help solve the transportation difficulties, potato is made into whole flour by many food processing companies. Potato whole flour has high nutritional value and may be regarded as beneficial to human health and fitness. In addition to the direct use of potato whole flour,it can also be used as added ingredients into other food products. This study summarizes and analyzes the processing technology of potato whole flour and its application in noodle products.展开更多
目的:利用基因集富集分析(gene set enrichment analysis,GSEA)和巨噬细胞炎症反应模型,基于通路水平阐释补肺健脾方干预慢性阻塞性肺疾病的配伍机制。方法:利用LPS诱导巨噬细胞建立炎症反应模型,分别给予补肺健脾方的12味中药干预,利用...目的:利用基因集富集分析(gene set enrichment analysis,GSEA)和巨噬细胞炎症反应模型,基于通路水平阐释补肺健脾方干预慢性阻塞性肺疾病的配伍机制。方法:利用LPS诱导巨噬细胞建立炎症反应模型,分别给予补肺健脾方的12味中药干预,利用Illumina高通量测序平台得到基因表达谱数据。采用GSEA方法鉴定巨噬细胞炎症反应相关通路,通过富集评分(normalized enrichment score,NES)筛选中药干预后显著回调的通路,揭示补肺健脾方及其配伍的干预机制。结果:与对照组比较,模型组筛选到244条显著扰动通路(GSEA,FDR<0.05)。以得到的显著扰动通路为对象,补肺健脾方所含中药显著回调通路的NES为-1041.56,其中补肺配伍的为-71.25,健脾配伍的为-17.45,温肾配伍的为-211.42,止咳化痰配伍的为-363.94,活血通络配伍的为-377.5;补肺健脾方可显著回调214条通路(逆转率为87.70%),其中补肺配伍的为43条,健脾配伍的为19条,温肾配伍的为133条,止咳化痰配伍的为142条,活血通络配伍的为187条,逆转率分别为17.62%、7.79%、54.51%、58.20%、76.64%。Positive regulation of interleukin 1 production等7条通路在5个配伍中均被显著回调。Mitotic cell cycle arrest等通路在补肺配伍中特异回调。Response to acetylcholine通路在健脾配伍中特异回调。Negative regulation of viral entry into host cell等通路在温肾配伍中特异回调。Negative regulation of leukocyte chemotaxis等通路在止咳化痰配伍中特异回调。Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors等通路在活血通络配伍中特异回调。结论:补肺健脾方各配伍回调炎症反应相关通路的强度及数量均依次为活血通络、止咳化痰、温肾、补肺、健脾配伍。补肺健脾方可通过各配伍回调positive regulation of interleukin 1 production等共性通路及negative regulation of leukocyte chemotaxis等特异性通路干预炎症反应。展开更多
OBJECTIVE:To explore the underlying mechanisms of the effects of Yangqing Chenfei formula(养清尘肺方,YCF) on inflammation and fibrosis in silicosis via inhibition of macrophage polarization.METHODS:A silicotic rat mod...OBJECTIVE:To explore the underlying mechanisms of the effects of Yangqing Chenfei formula(养清尘肺方,YCF) on inflammation and fibrosis in silicosis via inhibition of macrophage polarization.METHODS:A silicotic rat model was established via a single intratracheal instillation of silica particles on the first day of week 0.Subsequently,YCF was administered intragastrically to silicotic rats during weeks 0-2 and 5-8 twice daily.The mouse-derived alveolar macrophage cell line was used to investigate the mechanisms of YCF in M1/M2 polarization.RESULTS:YCF treatment effectively inhibited lung pathological changes,including inflammatory cell infiltration and tissue damage,and increased the forced expiratory volume in the first 0.3 s,functional residual capacity,and maximal mid-expiratory flow in weeks 2 and 8.Furthermore,the treatment improved lung functions by upregulating tidal volume,pause increase,and expiratory flow at 50% tidal volume from weeks 5 to 8.Moreover,YCF could significantly suppressed the progression of inflammation and fibrosis,by reducing the levels of inflammatory cytokines,as well as collagen-Ⅰ and Ⅲ.YCF treatment also decreased the numbers of macrophages and M1/M2 macrophages and the level of transforming growth factor-β(TGF-β).Additionally,YCF5,the effective substance in YCF,decreased lipopolysaccharide and interferon-γ-induced M1 macrophage polarization in a concentration-dependent manner.The mechanism of anti-M1 polarization might be related to a decrease in extracellular signal-regulated kinase,c-JUN N-terminal kinase,P38,and P65 phosphorylation.Furthermore,YCF5 inhibited interleukin-4-induced M2 macrophages by decreasing the protein and m RNA expressions of arginase-1 and CD206 as well as the levels of profibrotic factors,such as TGF-β and connective tissue growth factor.The mechanisms underlying the anti-M2 polarization of YCF5 were primarily associated with the inhibition of the nuclear translocation of phosphorylated signal transducer and activator of transcription 6(pSTAT6).CONCLUSION:YCF significantly inhibits inflammation and fibrosis in silicotic rats probably via the suppression of M1/M2 macrophage polarization mediated by the inhibition of mitogen-activated protein kinase and nuclear factor kappa B signaling pathways and Janus kinase/STAT6 pathways.展开更多
目的从通路水平探究慢性阻塞性肺疾病有效方药补肺益肾方的干预机制。方法采用LPS诱导巨噬细胞建立炎症反应模型。基于基因集富集分析(Gene set enrichment analysis,GSEA)方法,筛选巨噬细胞炎症反应相关通路,通过富集评分(Normalized e...目的从通路水平探究慢性阻塞性肺疾病有效方药补肺益肾方的干预机制。方法采用LPS诱导巨噬细胞建立炎症反应模型。基于基因集富集分析(Gene set enrichment analysis,GSEA)方法,筛选巨噬细胞炎症反应相关通路,通过富集评分(Normalized enrichment score,NES)筛选药物干预后发生逆转的通路,揭示补肺益肾方及其配伍的干预机制。结果补肺益肾方所含中药的NES为-1377.23,其中补肾配伍的为-485.07、活血配伍的为-351.86、化痰配伍的为-303.71、益气配伍的为-236.59;补肺益肾方显著逆转的通路为213条,其中活血配伍的为184条、补肾配伍的为147条、化痰配伍的为134条、益气配伍的为133条,逆转率分别为75.41%、60.25%、54.92%、54.51%。TGF-βproduction等90条通路在4个配伍中均被显著逆转。Positive regulation of cytokine production involved in inflammatory response等为配伍特异性逆转通路。结论补肺益肾方各配伍组逆转炎症信号通路的强度依次为补肾、活血、化痰、益气配伍,逆转通路数量依次为活血、补肾、化痰、益气。补肺益肾方可通过调控各配伍共性及特异性逆转通路干预炎症反应。展开更多
基金Supported by National Key R&D Program of China(2016YFD0401302)National Natural Science Foundation of China(31701636)
文摘When harvested the potato has a high moisture content, this could make its transportation and storage difficult. To help solve the transportation difficulties, potato is made into whole flour by many food processing companies. Potato whole flour has high nutritional value and may be regarded as beneficial to human health and fitness. In addition to the direct use of potato whole flour,it can also be used as added ingredients into other food products. This study summarizes and analyzes the processing technology of potato whole flour and its application in noodle products.
文摘目的:利用基因集富集分析(gene set enrichment analysis,GSEA)和巨噬细胞炎症反应模型,基于通路水平阐释补肺健脾方干预慢性阻塞性肺疾病的配伍机制。方法:利用LPS诱导巨噬细胞建立炎症反应模型,分别给予补肺健脾方的12味中药干预,利用Illumina高通量测序平台得到基因表达谱数据。采用GSEA方法鉴定巨噬细胞炎症反应相关通路,通过富集评分(normalized enrichment score,NES)筛选中药干预后显著回调的通路,揭示补肺健脾方及其配伍的干预机制。结果:与对照组比较,模型组筛选到244条显著扰动通路(GSEA,FDR<0.05)。以得到的显著扰动通路为对象,补肺健脾方所含中药显著回调通路的NES为-1041.56,其中补肺配伍的为-71.25,健脾配伍的为-17.45,温肾配伍的为-211.42,止咳化痰配伍的为-363.94,活血通络配伍的为-377.5;补肺健脾方可显著回调214条通路(逆转率为87.70%),其中补肺配伍的为43条,健脾配伍的为19条,温肾配伍的为133条,止咳化痰配伍的为142条,活血通络配伍的为187条,逆转率分别为17.62%、7.79%、54.51%、58.20%、76.64%。Positive regulation of interleukin 1 production等7条通路在5个配伍中均被显著回调。Mitotic cell cycle arrest等通路在补肺配伍中特异回调。Response to acetylcholine通路在健脾配伍中特异回调。Negative regulation of viral entry into host cell等通路在温肾配伍中特异回调。Negative regulation of leukocyte chemotaxis等通路在止咳化痰配伍中特异回调。Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors等通路在活血通络配伍中特异回调。结论:补肺健脾方各配伍回调炎症反应相关通路的强度及数量均依次为活血通络、止咳化痰、温肾、补肺、健脾配伍。补肺健脾方可通过各配伍回调positive regulation of interleukin 1 production等共性通路及negative regulation of leukocyte chemotaxis等特异性通路干预炎症反应。
基金Special Project of Traditional Chinese Medicine Research of Henan Province(2021ZYZD01)Evaluation of the Therapeutic Effect and Characteristics of Traditional Chinese Medicine Dialectical Treatment for Coal Worker's Pneumoconiosis Based on A Multicenter,Randomized,Double-Blind,Parallel Controlled Trial+1 种基金National Natural Science Fund of China(81973822)Exploring the Mechanism of Bufei Yishen Formula Inhibiting Inflammatory Response in the Treatment of COPD Based on Group Allocation Theory。
文摘OBJECTIVE:To explore the underlying mechanisms of the effects of Yangqing Chenfei formula(养清尘肺方,YCF) on inflammation and fibrosis in silicosis via inhibition of macrophage polarization.METHODS:A silicotic rat model was established via a single intratracheal instillation of silica particles on the first day of week 0.Subsequently,YCF was administered intragastrically to silicotic rats during weeks 0-2 and 5-8 twice daily.The mouse-derived alveolar macrophage cell line was used to investigate the mechanisms of YCF in M1/M2 polarization.RESULTS:YCF treatment effectively inhibited lung pathological changes,including inflammatory cell infiltration and tissue damage,and increased the forced expiratory volume in the first 0.3 s,functional residual capacity,and maximal mid-expiratory flow in weeks 2 and 8.Furthermore,the treatment improved lung functions by upregulating tidal volume,pause increase,and expiratory flow at 50% tidal volume from weeks 5 to 8.Moreover,YCF could significantly suppressed the progression of inflammation and fibrosis,by reducing the levels of inflammatory cytokines,as well as collagen-Ⅰ and Ⅲ.YCF treatment also decreased the numbers of macrophages and M1/M2 macrophages and the level of transforming growth factor-β(TGF-β).Additionally,YCF5,the effective substance in YCF,decreased lipopolysaccharide and interferon-γ-induced M1 macrophage polarization in a concentration-dependent manner.The mechanism of anti-M1 polarization might be related to a decrease in extracellular signal-regulated kinase,c-JUN N-terminal kinase,P38,and P65 phosphorylation.Furthermore,YCF5 inhibited interleukin-4-induced M2 macrophages by decreasing the protein and m RNA expressions of arginase-1 and CD206 as well as the levels of profibrotic factors,such as TGF-β and connective tissue growth factor.The mechanisms underlying the anti-M2 polarization of YCF5 were primarily associated with the inhibition of the nuclear translocation of phosphorylated signal transducer and activator of transcription 6(pSTAT6).CONCLUSION:YCF significantly inhibits inflammation and fibrosis in silicotic rats probably via the suppression of M1/M2 macrophage polarization mediated by the inhibition of mitogen-activated protein kinase and nuclear factor kappa B signaling pathways and Janus kinase/STAT6 pathways.
文摘目的从通路水平探究慢性阻塞性肺疾病有效方药补肺益肾方的干预机制。方法采用LPS诱导巨噬细胞建立炎症反应模型。基于基因集富集分析(Gene set enrichment analysis,GSEA)方法,筛选巨噬细胞炎症反应相关通路,通过富集评分(Normalized enrichment score,NES)筛选药物干预后发生逆转的通路,揭示补肺益肾方及其配伍的干预机制。结果补肺益肾方所含中药的NES为-1377.23,其中补肾配伍的为-485.07、活血配伍的为-351.86、化痰配伍的为-303.71、益气配伍的为-236.59;补肺益肾方显著逆转的通路为213条,其中活血配伍的为184条、补肾配伍的为147条、化痰配伍的为134条、益气配伍的为133条,逆转率分别为75.41%、60.25%、54.92%、54.51%。TGF-βproduction等90条通路在4个配伍中均被显著逆转。Positive regulation of cytokine production involved in inflammatory response等为配伍特异性逆转通路。结论补肺益肾方各配伍组逆转炎症信号通路的强度依次为补肾、活血、化痰、益气配伍,逆转通路数量依次为活血、补肾、化痰、益气。补肺益肾方可通过调控各配伍共性及特异性逆转通路干预炎症反应。
