The reduction of geraniol to citronellol is the first step for the synthesis of natural phytol in the production of tocopherols and natural vitamin K.Baker's yeast was used in the bioreduction described above as a...The reduction of geraniol to citronellol is the first step for the synthesis of natural phytol in the production of tocopherols and natural vitamin K.Baker's yeast was used in the bioreduction described above as a whole-cell biocatalyst.However,the enzyme responsible for the reduction of geraniol to citronellol is not yet known.Four old yellow enzyme(OYE)genes were cloned from yeast and plants,and expressed in Escherichia coli for a high level of recombinant proteins.The recombinant protein displayed a catalytic activity of converting geraniol to citronellol as a sole product verified by GC-MS analyses.The recombinant OYE2 intact cells were found to show 3.7 and 1.9-fold higher activity than that of yeast cells and the recombinant crude extracts,respectively.Compared to the recombinant fusion enzyme,the entrokinase-cleaved enzyme displayed nearly identical activity for geraniol reduction.To our knowledge,this is the first enzyme identified to catalyze the formation of citronellol from geraniol by reducing the allylic alcohol double bond,which is normally known as inactivating group for the old yellow enzymes.展开更多
Background:To observe the effect of perioperative acupuncture on postoperative pain in patients undergoing standard sternotomy.Methods:57 patients who received standard median sternotomy were randomly divided into acu...Background:To observe the effect of perioperative acupuncture on postoperative pain in patients undergoing standard sternotomy.Methods:57 patients who received standard median sternotomy were randomly divided into acupuncture group(30 cases)and control group(27 cases).The control group was treated with routine operation and nursing,and the acupuncture group was treated with acupuncture for 10 days on the basis of perioperative period.The pain levels of the two groups before and after operation were compared.Results:The pain level of the acupuncture group was significantly lower than that of the control group on the 2nd,10th,and 30th days after operation(P<0.05),and the short-term pain levels in the two groups were statistically significant(P<0.05).Conclusion:The perioperative acupuncture treatment has a significant analgesic effect on short-term pain for patients undergoing anterior midline thoracotomy,and it is helpful for patients to recover quickly after surgery.展开更多
基金This work was supported by grants from the Ministry of Science and Technology of China(2007CB108802)State Key Laboratory of Phytochemistry and Plant Resources in West China(P2008-ZZ16,P2009-ZZ02).
文摘The reduction of geraniol to citronellol is the first step for the synthesis of natural phytol in the production of tocopherols and natural vitamin K.Baker's yeast was used in the bioreduction described above as a whole-cell biocatalyst.However,the enzyme responsible for the reduction of geraniol to citronellol is not yet known.Four old yellow enzyme(OYE)genes were cloned from yeast and plants,and expressed in Escherichia coli for a high level of recombinant proteins.The recombinant protein displayed a catalytic activity of converting geraniol to citronellol as a sole product verified by GC-MS analyses.The recombinant OYE2 intact cells were found to show 3.7 and 1.9-fold higher activity than that of yeast cells and the recombinant crude extracts,respectively.Compared to the recombinant fusion enzyme,the entrokinase-cleaved enzyme displayed nearly identical activity for geraniol reduction.To our knowledge,this is the first enzyme identified to catalyze the formation of citronellol from geraniol by reducing the allylic alcohol double bond,which is normally known as inactivating group for the old yellow enzymes.
文摘Background:To observe the effect of perioperative acupuncture on postoperative pain in patients undergoing standard sternotomy.Methods:57 patients who received standard median sternotomy were randomly divided into acupuncture group(30 cases)and control group(27 cases).The control group was treated with routine operation and nursing,and the acupuncture group was treated with acupuncture for 10 days on the basis of perioperative period.The pain levels of the two groups before and after operation were compared.Results:The pain level of the acupuncture group was significantly lower than that of the control group on the 2nd,10th,and 30th days after operation(P<0.05),and the short-term pain levels in the two groups were statistically significant(P<0.05).Conclusion:The perioperative acupuncture treatment has a significant analgesic effect on short-term pain for patients undergoing anterior midline thoracotomy,and it is helpful for patients to recover quickly after surgery.
