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Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway 被引量:7
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作者 Juan DONG Guo ZHU +1 位作者 tian-cheng wang Fu-shan SHI 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2017年第5期445-448,共4页
目的:研究人参皂甙Rg1对小鼠脂肪干细胞神经样分化的促进作用,并初步探讨其作用机理。创新点:证明了人参皂甙Rg1可以通过mi RNA-124途径促进脂肪干细胞的神经样分化。方法:从BALB/c小鼠腹股沟和睾丸脂肪垫处分离培养脂肪干细胞,利用流... 目的:研究人参皂甙Rg1对小鼠脂肪干细胞神经样分化的促进作用,并初步探讨其作用机理。创新点:证明了人参皂甙Rg1可以通过mi RNA-124途径促进脂肪干细胞的神经样分化。方法:从BALB/c小鼠腹股沟和睾丸脂肪垫处分离培养脂肪干细胞,利用流式细胞仪检测分离的脂肪干细胞纯度。试验分为以下五组:磷酸缓冲盐溶液(PBS)组、3-异丁基-1-甲基黄嘌呤(IBMX)组、IBMX+Rg1低剂量组、IBMX+Rg1中剂量组和IBMX+Rg1高剂量组。用细胞免疫组化方法检测了脂肪干细胞向神经样细胞的分化效率,用荧光定量聚合酶链式反应(qP CR)方法检测mi RNA-124的表达变化,用免疫印迹的方法检测巢蛋白(nestin)、βIII-微管蛋白(βIII-tubulin)及羧基端小结构域磷酸酶1(SCP1)的表达水平。结论:免疫组化结果显示,IBMX可以成功诱导小鼠脂肪干细胞向神经样细胞的分化;免疫印迹结果显示,Rg1可以显著提高神经样细胞标记蛋白的表达水平;荧光定量PCR结果显示,Rg1可以促进miRNA-124的表达量,进而降解神经分化抑制因子SCP1的表达,促进脂肪干细胞的神经样分化效率。 展开更多
关键词 脂肪干细胞 人参皂甙RG1 神经样分化
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Evaluating and Constraining Hardware Assertions with Absent Scenarios
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作者 Hui-Na Chao Hua-Wei Li +2 位作者 Xiaoyu Song tian-cheng wang Xiao-Wei Li 《Journal of Computer Science & Technology》 SCIE EI CSCD 2020年第5期1198-1216,共19页
Mining from simulation data of the golden model in hardware design verification is an effective solution to assertion generation.While the simulation data is inherently incomplete,it is necessary to evaluate the truth... Mining from simulation data of the golden model in hardware design verification is an effective solution to assertion generation.While the simulation data is inherently incomplete,it is necessary to evaluate the truth values of the mined assertions.This paper presents an approach to evaluating and constraining hardware assertions with absent scenarios.A Belief-fail Rate metric is proposed to predict the truth/falseness of generated assertions.By considering both the occurrences of free variable assignments and the conflicts of absent scenarios,we use the metric to sort true assertions in higher ranking and false assertions in lower ranking.Our Belief-failRate guided assertion constraining method leverages the quality of generated assertions.The experimental results show that the Belief-failRate framework performs better than the existing methods.In addition,the assertion evaluating and constraining procedure can find more assertions that cover new design functionality in comparison with the previous methods. 展开更多
关键词 hardware formal verification assertion generation data mining assertion evaluation assertion coverage
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