Gamma-aminobutyric acid enhances miR-21-5p loading into adipose-derived stem cell extracellular vesicles to alleviate myocardial ischemia-reperfusion injury via TXNIP regulation

BACKGROUND Myocardial ischemia-reperfusion injury(MIRI)poses a prevalent challenge in current reperfusion therapies,with an absence of efficacious interventions to address the underlying causes.AIM To investigate whether the extracellular vesicles(EVs)secreted by adipose mesenchymal stem cells(ADSCs)derived from subcutaneous inguinal adipose tissue(IAT)underγ-aminobutyric acid(GABA)induction(GABA-EVs^(IAT))demonstrate a more pronounced inhibitory effect on mitochondrial oxidative stress and elucidate the underlying mechanisms.METHODS We investigated the potential protective effects of EVs derived from mouse ADSCs pretreated with GABA.We assessed cardiomyocyte injury using terminal deoxynucleotidyl transferase dUTP nick end-labeling and Annexin V/propidium iodide assays.The integrity of cardiomyocyte mitochondria morphology was assessed using electron microscopy across various intervention backgrounds.To explore the functional RNA diversity between EVs^(IAT)and GABA-EVs^(IAT),we employed microRNA(miR)sequencing.Through a dual-luciferase reporter assay,we confirmed the molecular mechanism by which EVs mediate thioredoxin-interacting protein(TXNIP).Western blotting and immunofluorescence were conducted to determine how TXNIP is involved in mediation of oxidative stress and mitochondrial dysfunction.RESULTS Our study demonstrates that,under the influence of GABA,ADSCs exhibit an increased capacity to encapsulate a higher abundance of miR-21-5p within EVs.Consequently,this leads to a more pronounced inhibitory effect on mitochondrial oxidative stress compared to EVs from ADSCs without GABA intervention,ultimately resulting in myocardial protection.On a molecular mechanism level,EVs regulate the expression of TXNIP and mitigating excessive oxidative stress in mitochondria during MIRI process to rescue cardiomyocytes.CONCLUSION Administration of GABA leads to the specific loading of miR-21-5p into EVs by ADSCs,thereby regulating the expression of TXNIP.The EVs derived from ADSCs treated with GABA effectively ameliorates mitochondrial oxidative stress and mitigates cardiomyocytes damage in the pathological process of MIRI.

A Study on the Impact of A spherical video-based virtual reality on Teaching English Writing Guided by Experiential Learning Circle Theory

English as an international language,English writing is a very important skill as an important form of language output,which is not only an important means of expressing emotions and awareness,but also reflects the ability to use language in an integrated way.In language teaching,the development of English writing skills is an important and challenging teaching task.English writing language is expressed not only at the basic level of vocabulary,grammar,and sentence structure,but also at the higher-order level of text organization,logical reasoning,and argumentation.However,in traditional English writing teaching,students usually do not have the opportunity to have an in-depth experience of the context of the topic,which leads to a lack of motivation and poor writing results.Therefore,this study developed an SVVR learning system based on spherical video-based virtual reality(SVVR)technology and conducted a quasi-experiment in a university English major classroom.The results of the study showed that the SVVR-supported approach to English writing could improve students'writing performance,enrich writing content,and increase learning motivation.

Review on the petroleum market in China:history,challenges and prospects

The petroleum industry plays an essential role in driving China's economic development.In the past few decades,severa reforms in the petroleum industry have been implemented;however,there are still some is sues that have not been resolved Moreover,with the new-normal economy,the transition to green energy and international trade disputes,the petroleum marke is also facing emerging challenges.Therefore,the purpose of the present study is to review the historical development o China's petroleum market,identify the current challenges and propose corresponding countermeasures for future prospects As a conclusion,five main challenges are highlighted totally,namely lack of marketization,excess oil refining capacity,hig external dependency,environment pollution and unstable international trading relationship.To address these challenges it is encouraged to deepen petroleum market reform,accelerate the elimination of inefficient refining capacity,diversify of supply sources,as well as improve domestic petroleum enterprises'ability to resist price risks.

Extracellular vesicles from hypoxia-preconditioned mesenchymal stem cells alleviates myocardial injury by targeting thioredoxininteracting protein-mediated hypoxia-inducible factor-1αpathway

BACKGROUND Extracellular vesicles(EVs)derived from hypoxia-preconditioned(HP)mesenchymal stem cells(MSCs)have better cardioprotective effects against myocardial infarction(MI)in the early stage than EVs isolated from normoxic(NC)-MSCs.However,the cardioprotective mechanisms of HP-EVs are not fully understood.AIM To explore the cardioprotective mechanism of EVs derived from HP MSCs.METHODS We evaluated the cardioprotective effects of HP-EVs or NC-EVs from mouse adipose-derived MSCs(ADSCs)following hypoxia in vitro or MI in vivo,in order to improve the survival of cardiomyocytes(CMs)and restore cardiac function.The degree of CM apoptosis in each group was assessed by the terminal deoxynucleotidyl transferase dUTP nick end-labeling and Annexin V/PI assays.MicroRNA(miRNA)sequencing was used to investigate the functional RNA diversity between HP-EVs and NC-EVs from mouse ADSCs.The molecular mechanism of EVs in mediating thioredoxin-interacting protein(TXNIP)was verified by the dual-luciferase reporter assay.Co-immunoprecipitation,western blotting,and immunofluorescence were performed to determine if TXNIP is involved in hypoxia-inducible factor-1 alpha(HIF-1α)ubiquitination and degradation via the chromosomal region maintenance-1(CRM-1)-dependent nuclear transport pathway.RESULTS HP-EVs derived from MSCs reduced both infarct size(necrosis area)and apoptotic degree to a greater extent than NC-EVs from CMs subjected to hypoxia in vitro and mice with MI in vivo.Sequencing of EV-associated miRNAs showed the upregulation of 10 miRNAs predicted to bind TXNIP,an oxidative stress-associated protein.We showed miRNA224-5p,the most upregulated miRNA in HP-EVs,directly combined the 3’untranslated region of TXNIP and demonstrated its critical protective role against hypoxia-mediated CM injury.Our results demonstrated that MI triggered TXNIP-mediated HIF-1αubiquitination and degradation in the CRM-1-mediated nuclear transport pathway in CMs,which led to aggravated injury and hypoxia tolerance in CMs in the early stage of MI.CONCLUSION The anti-apoptotic effects of HP-EVs in alleviating MI and the hypoxic conditions of CMs until reperfusion therapy may partly result from EV miR-224-5p targeting TXNIP.

Sensitivity Analysis and Selection of Check Index of Signal Intersection Simulation Model Based on VISSIM

Selecting check index quantitatively is the core of the calibration of micro traffic simulation parameters at signal intersection. Five indexes in the node (intersection) module of VISSIM were selected as the check index set. Twelve simulation parameters in the core module were selected as the simulation parameters set. Optimal process of parameter calibration was proposed and model of the intersection of Huangcun west street and Xinghua street in Beijing was built in VISSIM to verify it. The sensitivity analysis between each check index and simulation parameter in their own set was conducted respectively. Sensitive parameter sets of different check indices were obtained and compared. The results show that different indexes have different size of set, and average vehicle delay’s is maximum, so it’s necessary to select index quantitatively. The results can provide references for scientific selection of the check indexes and improve the study efficiency of parameter calibration.

Quantitative investigation of nanofluid imbibition in tight oil reservoirs based on NMR technique

Nanofluids have been effective chemical additives for enhanced oil recovery(EOR)in tight oil reservoirs due to their special properties.However,oil imbibition recoveries vary for different nanofluids.The oil/water distribution in rocks during imbibition using various nanofluids was less discussed in previous studies.In this study,we systematically examined the imbibition efficiencies of various nanofluids at60℃.Furthermore,the migration of nanofluids and oil distribution in the rock pores were monitored using nuclear magnetic resonance(NMR).The nanofluids were prepared by dispersing silica nanoparticles and five different types of surfactants i.e.,anionic-nonionic,anionic,nonionic,amphoteric and cationic surfactants in deionized(DI)water.Subsequently,interfacial tension(IFT)and contact angle measurements were conducted to reveal the underlying EOR mechanisms of various nanofluids.The experimental results showed that the EOR potential of the different types of nanofluids was in the order anionic-nonionic>anionic>nonionic>amphoteric>cationic>brine.Anionic-nonionic(sodium lauryl ether sulfate(SLES))and anionic(sodium dodecyl sulfonate(SDS))nanofluids exhibited excellent capability of wettability alteration,and increased oil recovery by 27.96%and 23.08%,respectively,compared to brine.The NMR results also showed that mesopores(0.1-1μm)were the dominant developed pores in the rocks,and contributed the most to imbibition efficiency.In addition,the imbibition of nanofluids initially took place in mesopores and micropores before moving into macropores.This study provides fundamental information on the selection of nanofluids for EOR in tight oil reservoirs.The study also improved the understanding of oil/water distribution during the imbibition of the proposed nanofluids.

Occurrence of lymphoma in non-gonadal organ during pregnancy: a report on four cases and literature review

Lymphoma rarely occurs during pregnancy, making this condition difficult to define. Lymphomas that occur in reproductive organs during pregnancy exhibit unique clinical characteristics. Among the limited cases, non-Hodgkin's lymphoma(NHL) shows a considerably higher incidence rate than Hodgkin's lymphoma(HL); NHL also displays clinical characteristics, such as high aggressiveness, advanced stage, and poor outcome. This study reports on four cases of lymphomas in non-gonadal organs(HL,n=2; NHL, n=2) during pregnancy. The tumors rapidly progressed in all patients during pregnancy but remitted at the end of pregnancy and/or therapy. The two HL cases were nodular sclerosis classical HL and treated with chemotherapy after terminating the pregnancy. One of the NHL cases was primary cutaneous follicular center lymphoma, a B cell-derived indolent lymphoma.The patient was followed up without any therapy after terminating her pregnancy. The other case was a follicular lymphoma grade3 B, which was treated with chemotherapy after delivery. We also conducted a literature review of 165 lymphoma cases occurring during pregnancy reported from 1976 to 2013 to reveal the correlation between pregnancy and lymphoma progression.Immunohistochemistry studies were performed to determine the expression of estrogen/progesterone receptors(ER/PR), and ER was weakly positive and sporadic. We concluded that lymphomas occurring during pregnancy should be managed with a prompt and reasonable treatment. High estrogen level in maternal body may affect lymphoma progression.

火箭基组合循环发动机总体布局研究进展（英文）

本综述从总体布局层面综述火箭基组合循环(RBCC)发动机在各个国家的发展现状,旨在展现该型发动机在单级入轨任务中的发展前景,为设计组合循环发动机以及进行空天往返任务规划提供参考。本文将RBCC按照构型特点进行归类并举例介绍,概述了发动机-机身一体化设计情况,并简要介绍了RBCC动力飞行器的任务规划和多目标优化方法。当前,尽管RBCC的研究面临着很多艰难的挑战,但是RBCC具有单级入轨的潜力,能够降低空天往返的成本。对RBCC发动机系统中各个子系统的研究也有利于促进其他相关学科的发展。

Eu^(3+)-doped Bovine Serum Albumin-derived Carbon Dots for Ratiometric Fluorescent Detection of Tetracycline

The abuse of antibiotics has become a global concern,thus it is significant to develop simple antibiotic assays.Herein,a novel type of lanthanide-doped bovine serum albumin(BSA)derived carbon dots(BCDs)dubbed Eu-BCDs has been unveiled for ratiometric fluorescent detection of tetracycline(TC),which was formed from the one-step hydrothermal reaction of Eu^(3+)and BSA.Upon challenge with TC,the blue fluorescence of Eu-BCDs at 448 nm was gradually quenched due to the internal filtration effect,while the characteristic red fluorescence of Eu^(3+)at 618 nm was significantly enhanced owning to the antenna effect,leading to the ratiometric fluorescent detection of TC with fast response(2 min),a wide linear dynamic range(0-80μmol/L),and high selectivity and sensitivity(limit of detection(LOD)of 3 nmol/L).Moreover,Eu-BCDs can be successfully applied for the ratiometric fluorescent detection of TC in environmental and biological samples,such as river water,milk,honey and serum.The Eu-BCDs sensing platform would have great application potential in view of its simple preparation,rapid response,high sensitivity and good selectivity.

大展弦比复合材料机翼结构设计与分析（英文）

目的:大展弦比机翼具有弯矩较大、扭转刚度较差的特点。在机翼结构上利用复合材料能很好地改善机翼结构性能。本文旨在设计一个满足刚度和强度要求的大展弦比复合材料机翼,并对大展弦比机翼遇到的大变形问题提出改进方案。创新点:1.通过流固耦合的方法对大展弦比机翼进行气动仿真和有限元静力分析;2.针对大展弦比机翼产生的大变形现象,提出增加机翼外挂或在翼尖处增加翼尖小翼的方法进行改进。方法:1.通过数值仿真建立机翼的有限元模型,并对机翼进行气动分析;2.通过流固耦合,将在FLUENT中的气动力加载到有限元静力分析模块进行分析;3.通过Workbench中的ACP复合材料专用模块,对复合材料结构进行铺层。结论:1.综合考虑刚度、强度以及减重效果,确定12 mm为本文大展弦比复合材料机翼的最佳蒙皮厚度;2.利用增加外挂的方法减小机翼大变形时,当外挂重心位置在机翼重心线前15%时机翼变形减小的程度最大;3.在翼尖处增加高度为300mm的翼尖小翼时机翼变形减小程度最大。4.在相同受载情况下,相比于金属材料机翼,复合材料机翼结构能够有效减小机翼的翼尖最大位移和最大应力。

Suppression mechanisms on proliferation of glioma U251 cells by FePt nanoparticles through intracellular oxidative stress

FePt nanoparticles(NPs)have attracted a great deal of attention in biomedical applications due to their excellent superparamagnetic property and anticancer activity,which display promising potential as the MRI/CT contrast imag-ing agent for cancer detection and therapy.In this study,the suppression mechanism induced by FePt NPs on U251 cells is investigated.Glioma U251 cells are co-cultured with FePt NPs at different concentrations,and the intra-cellular oxidative stress was studied by detecting the activities of various redox enzymes and expression of several apoptosis-related proteins.The cell viability assay shows that the highest suppression on the proliferation of glioma U251 cells is 10.5%±5.6%after treatment with 5μg·ml^(-1)FePt NPs for 24 h.In this case,the LDH release remarkably increases.Flow cytometry and fluorescence assay show that FePt NPs promote the apoptosis and G0/G1 phase arrest of U251 cells.

基于数值计算方法的翼型参数化、建模与优化研究（英文）

目的:1.比较并改善翼型参数化方法,获得设计变量少、拟合精度高的参数化方法;2.在参数化的基础上利用数值模拟的方法获取翼型流场参数,优化并获得特定条件下升阻比最大的翼型。创新点:1.通过与多项式拟合方法的对比证明了类别/形状函数转换(CST)法在翼型拟合方面的优越性,并通过调整控制点分布,在不增加设计变量的基础上改善了CST方法;2.通过建立响应面模型,利用多岛遗传算法与非线性序列二次规划法相结合的方式获得了更好的翼型优化效果。方法:1.利用修饰后的CST法对翼型进行参数化拟合与设计,并通过与二项式拟合法比较来验证其优越性;2.通过数值方法对翼型周围流场进行计算并与实验结果对比,获得精确计算气动参数的仿真条件;3.通过拉丁超立方采样获得设计变量,建立设计变量与翼型升阻比之间的响应面模型,通过多岛遗传算法与非线性序列二次规划法的结合和优化,得到一定条件下升阻比最大的翼型。结论:1.CST法是一种优秀的参数化方法,本文的优化改善了形状函数控制点选取法则,使其对翼型头部和尾部的描述更加精确;与多项式相比,CST法可以通过更少的设计变量得到更高的拟合精度。2.基于多岛遗传算法的非线性序列二次规划法在本文中用以优化翼型使其具有更高升阻比。优化前后翼型的比较显示,两种优化方法的结合可以得到比单独使用各优化方法更好的结果。

Immunogenicity of allogeneic mesenchymal stem cells Iransplanted via different routes in diabetic rats

Due to their hypoimmunogenicity and unique immunosuppressive properties, mesenchymal stem cells （MSCs） are considered one of the most promising adult stem cell types for cell therapy. Although many studies have shown that MSCs exert therapeutic effects on several acute and subacute conditions, their long-term effects are not confirmed in chronic diseases. Immunogenicity is a major limitation for cell replacement therapy, and it is not well understood in vivo. We evaluated the immunogenicity of allogeneic MSCs in vivoby transplanting MSCs into normal and diabetic rats viathe tail vein or pancreas and found that MSCs exhibited low immunogenicity in normal recipients and even exerted some immunosuppressive effects in diabetic rats during the initial phase. However, during the later stage in the pancreas group, MSCs expressed insulin and MHC II, eliciting a strong immune response in the pancreas. Simultaneously, the peripheral blood mononuclear cells in the recipients in the pancreas group were activated, and alloantibodies developed in vivo. Conversely, in the tail vein group, MSCs remained immunoprivileged and displayed immunosuppressive effects in vivo. These data indicate that different transplanting mutes and microenvironments can lead to divergent immunogenicity of MSCs.

Lie Symmetries,Conservation Laws and Explicit Solutions for Time Fractional Rosenau–Haynam Equation

Under investigation in this paper is the invariance properties of the time fractional Rosenau-Haynam equation, which can be used to describe the formation of patterns in liquid drops. By using the Lie group analysis method, the vector fields and symmetry reductions of the equation are derived, respectively. Moreover, based on the power series theory, a kind of explicit power series solutions for the equation are well constructed with a detailed derivation. Finally, by using the new conservation theorem, two kinds of conservation laws of the equation are well constructed with a detailed derivation.

The interaction between dietary marine components and intestinal flora

The intestine is the natural habitat for a large and dynamic bacterial community,which perform vital metabolic and immune functions that significantly effect the nutritional and health status of the host.The major physiological functions of intestinal flora include metabolic activities that lead to important trophic effects on immune structure and function as well as protection of the colonized host against invasion by transient microbes.The intestinal flora is both a target for nutritional intervention and a factor influencing the biological activity of food compounds acquired orally.Marine environments,as a source of diverse organic compounds,can supply us with a number of beneficial and health-promoting ingredients.This review focuses on the physiological functions of intestinal flora and reciprocal interactions between intestinal flora and dietary marine components including polysaccharides,lipids,protein,taurine,carotenoids,minerals,and polyphenols.This review can serve as a valuable reference regarding the consumption of seafood and marine nutraceuticals.