Effects of All-trans Retinoic Acid (ATRA) against Bacterial Infection in Chickens

In order to investigate the effects of all-trans retinoic acid （ATRA） against bacterial infection in chickens, 35 3-day-old AA broiler chickens were fed adaptively for two days and randomly divided into five groups, including Escherichia coli experimental group （ group 1 ）, Escherichia coli control group （group 2）, blank control group （ group 3 ）, PasteureUa experimental group （ group 4）, and PasteureUa control group （ group 5 ）. At 5 days of age, the chickens in group 1 and group 4 were drenched with 5 p.mol/kg ATRA for seven consecutive days according to their weight; the chickens in group 2, group 3 and group 5 were drenched with an equal volume of dimethyl sulfoxlde （DMSO）. The clinical symptoms and weight changes in each group were observed and recorded. Seven days later, the chickens were euthanized and dissected to determine the immune organ indexes. The results showed that there were significant differences in body weight between ATRA-administrated chickens and non-administrated chickens after bacterial infection （P 〈 0.05 ） ; moreover, the immune organ indexes of ATRA-administrated chickens exhibited significant differences compared with control group （P 〈 0.05 ）, indicating that ATRA could promote the development of immune organs of poultry, thereby enhancing the body immunity against bacterial infection.

Synthesis,characterization and cell imaging properties of rare earth compounds based on hydroxamate ligand

Six binuclear rare earth compounds were synthesized through the ligand of 4-Bromo-N-hydroxy-Nmethyl-benzamide（sH_2 bha）. Compounds 1-3 are isomorphous, and compound 1 is crystallized in the monoclinic system with the P2_1/c space group. Compounds 4 and 5 are isomorphous, and compound 4 is crystallized in the triclinic system with the P-1 space group. Compound 6 is crystallized in the triclinic system with the P-1 space group. The compounds were characterized by thermogravimetric analysis（TGA） and IR spectroscopy. The fluorescence spectrum in the visible area shows characteristic peaks of Eu, Tb, and Dy compounds. The efficiency of Eu compound on the viability of PC3 cells was assessed using CCK8 assays. From the CCK8 results, ionic concentration may have a great effect on PC3 cells＇ proliferation. Eu compound shows red fluorescence in the cytoplasm of PC3 cell. As for Eu compound, it might have potential application in cell imaging technology.