Anthocyanins are widely found in plants and are responsible for the purple coloration of plants. Anthocyanin biosynthesis is induced by environmental stresses, plant hormones, sugar, and so on. Tyrosine (Tyr) is the p...Anthocyanins are widely found in plants and are responsible for the purple coloration of plants. Anthocyanin biosynthesis is induced by environmental stresses, plant hormones, sugar, and so on. Tyrosine (Tyr) is the precursor of melanin that exits in both animals and plants. However, until now it has been unknown whether Tyr induces anthocyanin biosynthesis. In this study, the seedlings of Arabidopsis thaliana were treated with exogenous Tyr and then the anthocyanin accumulation was determined. The results showed that Tyr induced anthocyanin accumulation in Arabidopsis thaliana in a dose-dependent manner. Furthermore, the expression of the late anthocyanin biosynthetic genes including DFR, LDOX, and UF3GT, and the transcription factor genes PAP1, PAP2, and EGL3 was induced by Tyr. Taken together, these results demonstrated that Tyr is able to induce anthocyanin accumulation and suggested that Tyr up-regulates transcription factors PAP1, PAP2, and EGL3, which mediates the expression of the late anthocyanin biosynthetic genes and then induces anthocyanin biosynthesis.展开更多
Tyrosine (Tyr) and sucrose (Suc) play an important role in plant growth.However,the crosstalk between Tyr and Suc in regulating the plant growth has remained unclear.In this study,it was found that Suc is able to enha...Tyrosine (Tyr) and sucrose (Suc) play an important role in plant growth.However,the crosstalk between Tyr and Suc in regulating the plant growth has remained unclear.In this study,it was found that Suc is able to enhance Tyr-induced inhibition of growth in Arabidopsis seedlings.Both fresh weight and root length of Arabidopsis seedlings were significantly reduced when the medium was added with high concentrations (such as 0.5 or 1.0 mM)of Tyr whereas they were clearly increased with an addition of appropriate concentrations of Suc (such as 1 or 3%) in the medium,which suggested that the growth of Arabidopsis seedlings is inhibited by high concentrations of Tyr but promoted by appropriate concentrations of Suc.Interestingly,the inhibition of growth in Arabidopsis seedlings by 0.5 or 1.0 mM Tyr was significantly intensified once 1%or 3%of Suc was added in the medium.This study revealed a positive effect of Suc on the Tyr-induced inhibition of plant growth,and the results help us to further investigate the crosstalk of Suc and Tyr in the regulation of plant growth.展开更多
文摘Anthocyanins are widely found in plants and are responsible for the purple coloration of plants. Anthocyanin biosynthesis is induced by environmental stresses, plant hormones, sugar, and so on. Tyrosine (Tyr) is the precursor of melanin that exits in both animals and plants. However, until now it has been unknown whether Tyr induces anthocyanin biosynthesis. In this study, the seedlings of Arabidopsis thaliana were treated with exogenous Tyr and then the anthocyanin accumulation was determined. The results showed that Tyr induced anthocyanin accumulation in Arabidopsis thaliana in a dose-dependent manner. Furthermore, the expression of the late anthocyanin biosynthetic genes including DFR, LDOX, and UF3GT, and the transcription factor genes PAP1, PAP2, and EGL3 was induced by Tyr. Taken together, these results demonstrated that Tyr is able to induce anthocyanin accumulation and suggested that Tyr up-regulates transcription factors PAP1, PAP2, and EGL3, which mediates the expression of the late anthocyanin biosynthetic genes and then induces anthocyanin biosynthesis.
基金Supported by the Program for Key Basic Research of the Ministry of Science and Technology of China(2014CB160308)the National Science Foundation of China(31571802)
文摘Tyrosine (Tyr) and sucrose (Suc) play an important role in plant growth.However,the crosstalk between Tyr and Suc in regulating the plant growth has remained unclear.In this study,it was found that Suc is able to enhance Tyr-induced inhibition of growth in Arabidopsis seedlings.Both fresh weight and root length of Arabidopsis seedlings were significantly reduced when the medium was added with high concentrations (such as 0.5 or 1.0 mM)of Tyr whereas they were clearly increased with an addition of appropriate concentrations of Suc (such as 1 or 3%) in the medium,which suggested that the growth of Arabidopsis seedlings is inhibited by high concentrations of Tyr but promoted by appropriate concentrations of Suc.Interestingly,the inhibition of growth in Arabidopsis seedlings by 0.5 or 1.0 mM Tyr was significantly intensified once 1%or 3%of Suc was added in the medium.This study revealed a positive effect of Suc on the Tyr-induced inhibition of plant growth,and the results help us to further investigate the crosstalk of Suc and Tyr in the regulation of plant growth.
