Objective:Pancreatic ductal adenocarcinoma(PDAC)is an aggressive malignancy.CD8^(+)T cells,cancer stem cells(CSCs),and tumor budding(TB)have been significantly correlated with the outcome of patients with PDAC,but the...Objective:Pancreatic ductal adenocarcinoma(PDAC)is an aggressive malignancy.CD8^(+)T cells,cancer stem cells(CSCs),and tumor budding(TB)have been significantly correlated with the outcome of patients with PDAC,but the correlations have been independently reported.In addition,no integrated immune-CSC-TB profile for predicting survival in patients with PDAC has been established.Methods:Multiplexed immunofluorescence and artificial intelligence(AI)-based comprehensive analyses were used for quantification and spatial distribution analysis of CD8^(+)T cells,CD133^(+)CSCs,and TB.In vivo humanized patient-derived xenograft(PDX)models were established.Nomogram analysis,calibration curve,time-dependent receiver operating characteristic curve,and decision curve analyses were performed using R software.Results:The established‘anti-/pro-tumor’models showed that the CD8^(+)T cell/TB,CD8^(+)T cell/CD133^(+)CSC,TB-adjacent CD8^(+)T cell,and CD133^(+)CSC-adjacent CD8^(+)T cell indices were positively associated with survival of patients with PDAC.These findings were validated using PDX-transplanted humanized mouse models.An integrated nomogram-based immune-CSC-TB profile that included the CD8^(+)T cell/TB and CD8^(+)T cell/CD133^(+)CSC indices was established and shown to be superior to the tumor-nodemetastasis stage model in predicting survival of patients with PDAC.Conclusions:‘Anti-/pro-tumor’models and the spatial relationship among CD8^(+)T cells,CSCs,and TB within the tumor microenvironment were investigated.Novel strategies to predict the prognosis of patients with PDAC were established using AI-based comprehensive analysis and machine learning workflow.The nomogram-based immune-CSC-TB profile can provide accurate prognosis prediction for patients with PDAC.展开更多
Despite major improvements in cancer survival,metastasis is responsible for almost 90%of cancer-related mortality^(1,2).Nonetheless,the pathogenesis and molecular mechanisms of cancer metastasis remain poorly understood.
Pancreatic ductal adenocarcinoma(PDAC)is the leading cause of cancer-related death worldwide.As PDAC is more common in older adults and the population is aging,the incidence of pancreatic adenocarcinoma is expected to...Pancreatic ductal adenocarcinoma(PDAC)is the leading cause of cancer-related death worldwide.As PDAC is more common in older adults and the population is aging,the incidence of pancreatic adenocarcinoma is expected to increase in the coming years.As a result,the mechanism and clinical management of PDAC in the elderly population is receiving more attention.This review will discuss age-related morphological and pathological changes,clinical management,surgery and adjuvant therapies,and molecular changes in elderly PDAC patients.More research is needed to clarify molecular mechanisms and develop new prevention and treatment strategies for PDAC in elderly patients.展开更多
Highly virulent porcine epidemic diarrhea virus(PEDV)strains re-emerged and circulated in China at the end of 2010,causing significant economic losses in the pork industry worldwide.To understand the genetic dynamics ...Highly virulent porcine epidemic diarrhea virus(PEDV)strains re-emerged and circulated in China at the end of 2010,causing significant economic losses in the pork industry worldwide.To understand the genetic dynamics of PEDV during its passage in vitro,the PEDV G2 strain FJzzl was serially propagated in Vero cells for up to 200 passages.The susceptibility and adaptability of the FJzzl strain increased gradually as it was serially passaged in vitro.Sequence analysis revealed that amino acid(aa)changes were mainly concentrated in the S glycoprotein,which accounted for 72.22%-85.71%of all aa changes.A continuous aa deletion(^(55)I^(56)G^(57)E→^(55)K^(56)Δ^(57)Δ)occurred in the N-terminal domain of S1(Sl-NTD).To examine how the aa changes affected its virulence,FJzzl-F20 and FJzzl-F200 were selected to simultaneously evaluate their pathogenicity in suckling piglets.All the piglets in the FJzzl-F20-infected group showed typical diarrhea at 24 h postinfection,and the piglets died successively by 48 h postinfection.However,the clinical signs of the piglets in the FJzzl-F200-infected group were significantly weaker,and no deaths occurred.The FJzzl-F200-infected group also showed a lower level of fecal viral shedding and lower viral loads in the intestinal tissues,and no obvious histopathological lesions.TypeⅠandⅢinterferon were induced in the FJzzl-F200 infection group,together with pro-inflammatory cytokines,such as TNF-α,IL-1βand IL-8.These results indicate that the identified genetic changes may contribute to the attenuation of FJzzl strain,and the attenuated FJzzl-F200 may have the potential for developing PEDV live-attenuated vaccines.展开更多
基金supported by The Science&Technology Development Fund of Tianjin Education Commission for Higher Education(Grant No.2017KJ198)。
文摘Objective:Pancreatic ductal adenocarcinoma(PDAC)is an aggressive malignancy.CD8^(+)T cells,cancer stem cells(CSCs),and tumor budding(TB)have been significantly correlated with the outcome of patients with PDAC,but the correlations have been independently reported.In addition,no integrated immune-CSC-TB profile for predicting survival in patients with PDAC has been established.Methods:Multiplexed immunofluorescence and artificial intelligence(AI)-based comprehensive analyses were used for quantification and spatial distribution analysis of CD8^(+)T cells,CD133^(+)CSCs,and TB.In vivo humanized patient-derived xenograft(PDX)models were established.Nomogram analysis,calibration curve,time-dependent receiver operating characteristic curve,and decision curve analyses were performed using R software.Results:The established‘anti-/pro-tumor’models showed that the CD8^(+)T cell/TB,CD8^(+)T cell/CD133^(+)CSC,TB-adjacent CD8^(+)T cell,and CD133^(+)CSC-adjacent CD8^(+)T cell indices were positively associated with survival of patients with PDAC.These findings were validated using PDX-transplanted humanized mouse models.An integrated nomogram-based immune-CSC-TB profile that included the CD8^(+)T cell/TB and CD8^(+)T cell/CD133^(+)CSC indices was established and shown to be superior to the tumor-nodemetastasis stage model in predicting survival of patients with PDAC.Conclusions:‘Anti-/pro-tumor’models and the spatial relationship among CD8^(+)T cells,CSCs,and TB within the tumor microenvironment were investigated.Novel strategies to predict the prognosis of patients with PDAC were established using AI-based comprehensive analysis and machine learning workflow.The nomogram-based immune-CSC-TB profile can provide accurate prognosis prediction for patients with PDAC.
基金supported by the National Key Research and Development Program of China(2021YFA1201100)National Natural Science Foundation of China(Grant Nos.82072716,81802433,82030092,81720108028,82072657,82072659,82173295,81871968,81871978,and 82103006)+2 种基金Tianjin Natural Science Foundation(Grant No.20JCQNJC01330)Collaboration Program of Beijing Tianjin and Hebei(Grant No.22JCZXJC00120)Tianjin Research Innovation Project for Postgraduate Students(Grant No.2021YJSB262)。
文摘Despite major improvements in cancer survival,metastasis is responsible for almost 90%of cancer-related mortality^(1,2).Nonetheless,the pathogenesis and molecular mechanisms of cancer metastasis remain poorly understood.
基金supported by the National Key Research and Development Program of China(2021YFA1201100)the National Natural Science Foundation of China(grants 82072716,82030092)+1 种基金Collaboration Program of Beijing,Tianjin and Hebei(grant 22JCZXJC00120)Tianjin Research Innovation Project for Postgraduate Students(2021YJSB262).
文摘Pancreatic ductal adenocarcinoma(PDAC)is the leading cause of cancer-related death worldwide.As PDAC is more common in older adults and the population is aging,the incidence of pancreatic adenocarcinoma is expected to increase in the coming years.As a result,the mechanism and clinical management of PDAC in the elderly population is receiving more attention.This review will discuss age-related morphological and pathological changes,clinical management,surgery and adjuvant therapies,and molecular changes in elderly PDAC patients.More research is needed to clarify molecular mechanisms and develop new prevention and treatment strategies for PDAC in elderly patients.
基金supported by the National Program on Key Research Project of China(2016YFD0500100)the Shanghai Youth Scientific and Technological Yang Fan Program Grant(20YF1457800)+3 种基金the National Natural Science Foundation of China(31472207)the earmarked fund for Modern Agro-industry Technology Research System of China(CARS-36)the China Postdoctoral Science Foundation(2020M670555)Shanghai Minhang District talent development special funds。
文摘Highly virulent porcine epidemic diarrhea virus(PEDV)strains re-emerged and circulated in China at the end of 2010,causing significant economic losses in the pork industry worldwide.To understand the genetic dynamics of PEDV during its passage in vitro,the PEDV G2 strain FJzzl was serially propagated in Vero cells for up to 200 passages.The susceptibility and adaptability of the FJzzl strain increased gradually as it was serially passaged in vitro.Sequence analysis revealed that amino acid(aa)changes were mainly concentrated in the S glycoprotein,which accounted for 72.22%-85.71%of all aa changes.A continuous aa deletion(^(55)I^(56)G^(57)E→^(55)K^(56)Δ^(57)Δ)occurred in the N-terminal domain of S1(Sl-NTD).To examine how the aa changes affected its virulence,FJzzl-F20 and FJzzl-F200 were selected to simultaneously evaluate their pathogenicity in suckling piglets.All the piglets in the FJzzl-F20-infected group showed typical diarrhea at 24 h postinfection,and the piglets died successively by 48 h postinfection.However,the clinical signs of the piglets in the FJzzl-F200-infected group were significantly weaker,and no deaths occurred.The FJzzl-F200-infected group also showed a lower level of fecal viral shedding and lower viral loads in the intestinal tissues,and no obvious histopathological lesions.TypeⅠandⅢinterferon were induced in the FJzzl-F200 infection group,together with pro-inflammatory cytokines,such as TNF-α,IL-1βand IL-8.These results indicate that the identified genetic changes may contribute to the attenuation of FJzzl strain,and the attenuated FJzzl-F200 may have the potential for developing PEDV live-attenuated vaccines.
基金supported by the National Key R&D Program of China(2018YFA0506900,2018YFA0800901)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12030211)the National Natural Science Foundation of China(31671226,31871194)。