Clustered regularly interspaced short palindromic repeat(CRISPR)technologies have opened new scientific avenues widely used in biomedical research.But simple and efficient strategies to reversibly control CRISPR are l...Clustered regularly interspaced short palindromic repeat(CRISPR)technologies have opened new scientific avenues widely used in biomedical research.But simple and efficient strategies to reversibly control CRISPR are lacking.In contrast to previous methods of attaching molecules to the ribose of guide RNAs(gRNAs),we focused on molecules that can directly react with nucleobases.Here,we developed a new strategy to switch off the CRISPR system by efficiently installing 4-(bromomethyl)phenylboronic acid onto nucleobases in gRNAs.CRISPR can then be activated by hydrogen peroxide(H_(2)O_(2)).Collectively,this work demonstrates boronic acid reversibly modulating CRISPR systems through a H_(2)O_(2)-responsive manner.展开更多
基金the National Natural Science Foundation of China(grant nos.22177089,91853119,21721005,91753201,21877086,and 22177088)the Hubei Natural Science Foundation for Distinguished Young Scholars(grant no.2019CFA064)the Fundamental Research Funds for the Central Universities(grant no.2042019-kf0189).
文摘Clustered regularly interspaced short palindromic repeat(CRISPR)technologies have opened new scientific avenues widely used in biomedical research.But simple and efficient strategies to reversibly control CRISPR are lacking.In contrast to previous methods of attaching molecules to the ribose of guide RNAs(gRNAs),we focused on molecules that can directly react with nucleobases.Here,we developed a new strategy to switch off the CRISPR system by efficiently installing 4-(bromomethyl)phenylboronic acid onto nucleobases in gRNAs.CRISPR can then be activated by hydrogen peroxide(H_(2)O_(2)).Collectively,this work demonstrates boronic acid reversibly modulating CRISPR systems through a H_(2)O_(2)-responsive manner.
