Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antim...Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antimicrobial and anti-inflammatory activity of the hydroethanolic extract of Kalanchoe pinnata (Lam.) Pers. leaves. After phytochemicals screening, the content of phenolic compounds, proanthocyanidol and flavonoids in the extract of this plant was determined spectrophotometrically. Antimicrobial activity was assessed using the micro-dilution technique on 96-well plates in liquid medium, combined with agar spreading. Anti-inflammatory activity was assessed using the 1% carrageenan induced rat paw oedema model. Phytochemical screening revealed the presence of alkaloids, saponins, triterpenes and sterols, phenols and flavonoids in the plant extract in varying proportions. The extract contained (0.049 ± 0.03 µg EAG/mg extract) total polyphenols, (0.215 ± 0.025 µg CE/mg extract) proanthocyanidins and (385.435 ± 0.0328 µg ER/mg ES) flavonoids. The hydroethanolic extract of the leaves of this plant inhibited the in vitro growth of the microbial strains studied to varying degrees. The MIC of the extract varied from 12.5 to 25 mg/mL and the BMC from 12.5 to 50 mg/mL. The plant did not show any activity on 1% carrageenan-induced rat paw edema.展开更多
This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated Decembe...This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated December 17, 2001 and n˚407 dated October 3, 2008 respectively. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazolyl-2-yl-2,5-diphenyltetrazolium bromide (MTT) test. No significant toxicity was observed after 14 and 28 days, although a dose-dependent decrease in creatinemia was observed in male rats (the recipe to be used to moderate creatinine levels). Cytotoxicity was without effect on NCM 365 cells. The results obtained justify the use of the recipe in traditional medicine.展开更多
文摘Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antimicrobial and anti-inflammatory activity of the hydroethanolic extract of Kalanchoe pinnata (Lam.) Pers. leaves. After phytochemicals screening, the content of phenolic compounds, proanthocyanidol and flavonoids in the extract of this plant was determined spectrophotometrically. Antimicrobial activity was assessed using the micro-dilution technique on 96-well plates in liquid medium, combined with agar spreading. Anti-inflammatory activity was assessed using the 1% carrageenan induced rat paw oedema model. Phytochemical screening revealed the presence of alkaloids, saponins, triterpenes and sterols, phenols and flavonoids in the plant extract in varying proportions. The extract contained (0.049 ± 0.03 µg EAG/mg extract) total polyphenols, (0.215 ± 0.025 µg CE/mg extract) proanthocyanidins and (385.435 ± 0.0328 µg ER/mg ES) flavonoids. The hydroethanolic extract of the leaves of this plant inhibited the in vitro growth of the microbial strains studied to varying degrees. The MIC of the extract varied from 12.5 to 25 mg/mL and the BMC from 12.5 to 50 mg/mL. The plant did not show any activity on 1% carrageenan-induced rat paw edema.
文摘This work consisted of assessing the toxicity of a recipe of three plants used in traditional Togolese medicine. Acute and sub-acute toxicity was assessed according to OECD chemical test guidelines n˚423 dated December 17, 2001 and n˚407 dated October 3, 2008 respectively. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazolyl-2-yl-2,5-diphenyltetrazolium bromide (MTT) test. No significant toxicity was observed after 14 and 28 days, although a dose-dependent decrease in creatinemia was observed in male rats (the recipe to be used to moderate creatinine levels). Cytotoxicity was without effect on NCM 365 cells. The results obtained justify the use of the recipe in traditional medicine.
