Objective: To observe cyclooxygenase (COX)-2 expression in normal oral mucosa (NOM), oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) and explore its significance in the incidence of oral cancer. Metho...Objective: To observe cyclooxygenase (COX)-2 expression in normal oral mucosa (NOM), oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) and explore its significance in the incidence of oral cancer. Methods: The immunohistochemical method and RT-PCR method were applied to detect the expression of COX-2 and MMP-7 in 10 cases with NOM, 33 cases of with OLP and 38 cases with OSCC. Results: The expression of COX-2 mRNA in OSCC tissues (68.4%, 26/38) was significantly higher than in the OLP (24.2%, 8/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 mRNA in OSCC tissues (65.8%, 25/38) was significantly higher than in the OLP (30.3%, 10/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 in OLP was significantly higher than in the NOM ( P<0.05). There was no significant expression of COX-2 protein in NOM, and the positive rate was 42.4% (14/33) and 89.5% (34/38) in OLP and OSCC group, respectively. The COX-2 expression in cancer tissues was significantly higher than in NOM and OLP ( P<0.05). The MMP-7 protein expression in cancer tissues (84.2%, 32/38) was significantly higher than in NOM (10.0%, 1/10) and in OLP (42.4%, 14/33), and the positive rate in OLP was significantly higher than in NOM ( P<0.01). The COX-2 expression was associated with clinical stage ( P<0.05), the MMP-7 expression was associated with clinical stage and lymph node metastasis ( P<0.05). The expressions of COX-2 and MMP-7 mRNA were positively correlated with OSCC. Conclusions: The abnormal expressions of COX-2 and MMP-7 are closely related to the biological behavior of OSCC, the MMP-7 may be induced by COX-2, and further lead to the invasion and metastasis of OSCC.展开更多
A multi-modal action control approach is proposed for an autonomous soccer robot when the bottom hardware is unchangeable. Different from existing methods, the proposed control approach defines actions with the princi...A multi-modal action control approach is proposed for an autonomous soccer robot when the bottom hardware is unchangeable. Different from existing methods, the proposed control approach defines actions with the principle of "perception-planning-action" inspired by human intelligence. Character extraction is used to divide the perception input into different modes. Different control modes are built by combining different control methods for the linear velocity and angular velocity. Based on production rules, the motion control is realized by connecting different perceptions to the corresponding control mode. Simulation and real experiments are conducted with the middle-sized robot Frontier-I, and the proposed method is compared with a proportional-integral-derivative (PID) control method to display its feasibility and performance. The results show that the multi-modal action control method can make robots react rapidly in a dynamic environment.展开更多
AIM: To investigate whether the recombinant adenovirus induces the TNF-α-mediated apoptosis in vivo.METHODS: Human hepatocarcinoma cell line (HepG2) cells were transfected into BALB/c nude mice, and the tumor growth ...AIM: To investigate whether the recombinant adenovirus induces the TNF-α-mediated apoptosis in vivo.METHODS: Human hepatocarcinoma cell line (HepG2) cells were transfected into BALB/c nude mice, and the tumor growth curve was drawn. We analyzed apoptosis in HepG2 cells by TUNEL, HE staining and electron microscopy.RESULTS: AdIκBαM was expressed stably and eff iciently in HepG2 and could not be degraded by induction of TNF-α. Tumor growth in mice could be reduced remarkably if treated by AdIκBαM plus TNF-α. There was apoptosis of > 70% of cells treated with AdIκBαM plus TNF-α and about 50% of cells treated with AdIκBαM. In contrast, there was few cell apoptosis in HepG2 cells treated with phosphate buffered saline and AdIκBα. HepG2 cells in mice also exhibited a high level of apoptosis after in vivo injection with AdIκBαM. The tumor growth curve indicated the tumor transfected with AdIκBαM could be restrained.CONCLUSION: AdIκBαM gene therapy greatly enhances apoptosis due to inhibition of an NF-κB-mediated antiapoptosis signaling pathway.展开更多
Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral m...Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progression, RNA sequencing (RNA-Seq) was performed using samples from KCOT and primary stromal fibroblasts isolated from gingival tissues. Seven candidate genes that possess a function potentially related to KCOT progression were selected and their expression levels were confirmed by quantitative PCR, immunohistochemistry and enzyme-linked immunosorbent assay. Expression of lysyl oxidase-like 4 (LOXL4), the only candidate gene that encodes a secreted protein, was enhanced at both the mRNA and protein levels in KCOT stromal tissues and primary KCOT stromal fibroblasts compared to control tissues and primary fibroblasts (P〈0.05). In vitro, high expression of LOXL4 could enhance proliferation and migration of the human umbilical vein endothelial cells (HUVECs). There was a significant, positive correlation between LOXL4 protein expression and MVD in stroma of KCOT and control tissues (r=0.882). These data suggest that abnormal expression of LOXL4 of KCOT may enhance angiogenesis in KCOT, which may help to promote the locally aggressive biological behavior of KCOT.展开更多
Objective:To observe the effects of different anesthesia depths on the level of plasma cortisol during operation and the score of postoperative MMSE in elderly patients underwent radical gastrectomy.Methods: A total o...Objective:To observe the effects of different anesthesia depths on the level of plasma cortisol during operation and the score of postoperative MMSE in elderly patients underwent radical gastrectomy.Methods: A total of eighty seven elderly patients undergoing radical gastrectomy were enrolled in this study. Continuous infusion of propofol and remifentanil for anesthesia and maintenance of propofol were divided into two groups: BIS-I (BIS: 45-55) and BIS-II (BIS: 55-65) by adusting the doses of propofol. The data were recorded as following, thewhole dosage of propofol, the time of extubation and the score of VAS. The level of plasma cortisol was finished at the time of incision and tracheal intubation. MMSE scores were recorded on the day before operation and follow-up at 3 and 7 d postoperatively. Record the patient's operation to discharge time, postoperative complications.Results: The concentration of cortisol in BIS-I group was higher than that in BIS-II group. There was no significant difference in extubation time, extubation VAS score and MMSE score after operation.Conclusion:There were no significant differences in the BIS values between 45 and 65 for the elderly patients undergoing upper abdominal surgery, and there were no significant differences in the patients' postoperative stress, wake, extubation, early postoperative pain scores and postoperative cognitive function. BIS values in 45-55 compared to 55-65 patients with low plasma cortisol levels may be associated with better control of stress levels, to maintain the BIS value between 45-55 anesthesia depths is more reasonable.展开更多
Smooth and compact nickel layers were successfully prepared in the ionic liquid of 1-butyl-3-methylimidazolium tetrafluoroborate(BMIMBF4)by constant current electrodeposition method.The effects of temperature,additive...Smooth and compact nickel layers were successfully prepared in the ionic liquid of 1-butyl-3-methylimidazolium tetrafluoroborate(BMIMBF4)by constant current electrodeposition method.The effects of temperature,additive content,current density,and deposition time on the performance of the nickel layers were systematically analyzed.Scanning electron microscopy(SEM)and X-ray diffraction(XRD)were used to analyze the surface morphology and composition of the nickel layers.Meanwhile,the tensile strengths of the nickel layers were tested by universal tensile testing machine.The results show that different process conditions have a great effect on morphology and performance of the electrodeposited nickel layer.The optimization of process parameters is as follows:BMIMBF4 to ethylene glycol(EG)volume ratio of 2:1,deposition temperature of 120℃,and current density of 1.2 mA·cm^(-2).Current density has a greater influence on the tensile strength of the nickel layer,and the maximum value of tensile strength is 1275 MPa.展开更多
Molybdenum is a kind of refractory metal and can hardly be deposited in aqueous solution since it is more likely to form molybdenum oxide rather than elementary Mo,but molten salts and ionic liquid become alternative ...Molybdenum is a kind of refractory metal and can hardly be deposited in aqueous solution since it is more likely to form molybdenum oxide rather than elementary Mo,but molten salts and ionic liquid become alternative to obtain Mo layer.This paper shows an available process to obtain metallic state molybdenum deposit from ionic liquid.Mo layer was electrodeposited under constant current in ionic liquid 1-Butyl-3-methylimidazolium tetrafluoroborate(BMIMBF_4).Micromorphology of the electrodeposited Mo layer was observed by scanning electron microscope(SEM),energy dispersive spectroscopy(EDS)and X-ray photoelectron spectroscopy(XPS).Results show that elementary Mo layer was electrodeposited under constant current in ionic liquid BMIMBF4.Properties of the Mo layer are affected significantly by additive ethylene glycol(EG),deposition temperature and current density.By parallel tests taking into consideration of different technical factors,optimized process parameters are obtained as volume ratio of 2:1 in BMIMBF4+EG system,deposition temperature of 150℃and current density of 0.75 mA·cm^(-2).展开更多
In order to prepare electroformed nickel with excellent properties,the effect of different waveforms on microstructure and mechanical properties was investigated by optical microscopy(OM),X-ray diffraction(XRD) and te...In order to prepare electroformed nickel with excellent properties,the effect of different waveforms on microstructure and mechanical properties was investigated by optical microscopy(OM),X-ray diffraction(XRD) and tensile test,respectively.The results show that the samples are composed of micron columnar grains with high-density nanoscale coherent twin boundaries which are parallel to the growth plane.The tensile strength and elongation display opposite tendency with the increase in current density when direct current(DC) electroforming was applied.However,under the condition of pulse current(unidirectional,bidirectional) electroforming,the change regulation of tensile strength and elongation is close while cathode(peak,positive peak) current density increases.XRD results show that electroformed nickel layers with high purity are all fcc crystal configuration under different conditions of the current waveform.The nickel layers formed under bidirectional pulse current behave superior global performance with tensile strength of 692.0 MPa and elongation of 27.6 %.展开更多
目的:验证日粮中粗蛋白质浓度对断奶仔猪氨基酸转运载体的影响效应。创新点:从营养物质感应体的角度分析采用低氮日粮的可行性,并探索营养限制对营养转运体的影响及它们间的相互作用。方法:十八头初始体重9.57公斤的断奶仔猪被随机分为...目的:验证日粮中粗蛋白质浓度对断奶仔猪氨基酸转运载体的影响效应。创新点:从营养物质感应体的角度分析采用低氮日粮的可行性,并探索营养限制对营养转运体的影响及它们间的相互作用。方法:十八头初始体重9.57公斤的断奶仔猪被随机分为三组(每组6个重复),分别饲喂含有14%、17%和20%的粗蛋白日粮45天。按照理想蛋白质模型,日粮分别添加赖氨酸、蛋氨酸+半胱氨酸、苏氨酸和色氨酸来满足断奶仔猪的需要,整个试验期自由采食。试验结束后,屠宰仔猪并采集小肠样品。与14%粗蛋白组相比,空肠中ASCT2、4F2hc和ATB0 m RNA表达在17%和20%粗蛋白水平组分别上升23.00%、12.00%、6.00%和48.00%、47.00%、56.00%。结论:结果表明,14%粗蛋白水平组外源添加合成氨基酸并不能促进氨基酸转运载体增加氨基酸的吸收来满足断奶仔猪的生长性能。然而,17%粗蛋白水平组可以减少氮排放到环境中,同时又能满足此阶段断奶仔猪的生长发育。因此,17%粗蛋白水平组对这个阶段断奶仔猪最合适。展开更多
基金supported by Jinan Science and Technology Development Plans Grant (No.201121040)
文摘Objective: To observe cyclooxygenase (COX)-2 expression in normal oral mucosa (NOM), oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC) and explore its significance in the incidence of oral cancer. Methods: The immunohistochemical method and RT-PCR method were applied to detect the expression of COX-2 and MMP-7 in 10 cases with NOM, 33 cases of with OLP and 38 cases with OSCC. Results: The expression of COX-2 mRNA in OSCC tissues (68.4%, 26/38) was significantly higher than in the OLP (24.2%, 8/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 mRNA in OSCC tissues (65.8%, 25/38) was significantly higher than in the OLP (30.3%, 10/33) and NOM (0.0%, 0/10) ( P<0.01). The expression of MMP-7 in OLP was significantly higher than in the NOM ( P<0.05). There was no significant expression of COX-2 protein in NOM, and the positive rate was 42.4% (14/33) and 89.5% (34/38) in OLP and OSCC group, respectively. The COX-2 expression in cancer tissues was significantly higher than in NOM and OLP ( P<0.05). The MMP-7 protein expression in cancer tissues (84.2%, 32/38) was significantly higher than in NOM (10.0%, 1/10) and in OLP (42.4%, 14/33), and the positive rate in OLP was significantly higher than in NOM ( P<0.01). The COX-2 expression was associated with clinical stage ( P<0.05), the MMP-7 expression was associated with clinical stage and lymph node metastasis ( P<0.05). The expressions of COX-2 and MMP-7 mRNA were positively correlated with OSCC. Conclusions: The abnormal expressions of COX-2 and MMP-7 are closely related to the biological behavior of OSCC, the MMP-7 may be induced by COX-2, and further lead to the invasion and metastasis of OSCC.
基金Supported in part by research grants from the National Natural Science Foundation of China,No.30300298the National Natural Science Foundation of China's Joint Research Fund for Overseas Chinese Young Scholars,No.30228026
基金supported by National Natural Science Foundation of China (No.60443004)Science and Technology Project of CQ Education Committee (No.KJ080621)
文摘A multi-modal action control approach is proposed for an autonomous soccer robot when the bottom hardware is unchangeable. Different from existing methods, the proposed control approach defines actions with the principle of "perception-planning-action" inspired by human intelligence. Character extraction is used to divide the perception input into different modes. Different control modes are built by combining different control methods for the linear velocity and angular velocity. Based on production rules, the motion control is realized by connecting different perceptions to the corresponding control mode. Simulation and real experiments are conducted with the middle-sized robot Frontier-I, and the proposed method is compared with a proportional-integral-derivative (PID) control method to display its feasibility and performance. The results show that the multi-modal action control method can make robots react rapidly in a dynamic environment.
基金Supported by the Foundation of Hi-tech Research and Develop-ment Program of China (863 Program), No. 2001AA217121Co-fi rst-author: Li-Ping Jia
文摘AIM: To investigate whether the recombinant adenovirus induces the TNF-α-mediated apoptosis in vivo.METHODS: Human hepatocarcinoma cell line (HepG2) cells were transfected into BALB/c nude mice, and the tumor growth curve was drawn. We analyzed apoptosis in HepG2 cells by TUNEL, HE staining and electron microscopy.RESULTS: AdIκBαM was expressed stably and eff iciently in HepG2 and could not be degraded by induction of TNF-α. Tumor growth in mice could be reduced remarkably if treated by AdIκBαM plus TNF-α. There was apoptosis of > 70% of cells treated with AdIκBαM plus TNF-α and about 50% of cells treated with AdIκBαM. In contrast, there was few cell apoptosis in HepG2 cells treated with phosphate buffered saline and AdIκBα. HepG2 cells in mice also exhibited a high level of apoptosis after in vivo injection with AdIκBαM. The tumor growth curve indicated the tumor transfected with AdIκBαM could be restrained.CONCLUSION: AdIκBαM gene therapy greatly enhances apoptosis due to inhibition of an NF-κB-mediated antiapoptosis signaling pathway.
基金supported by the National Natural Science Foundation of China (grant nos. 81030018, 30872900 and 30901680)the Doctoral Fund of Ministry of Education of China (grant no. 20120001110043)
文摘Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progression, RNA sequencing (RNA-Seq) was performed using samples from KCOT and primary stromal fibroblasts isolated from gingival tissues. Seven candidate genes that possess a function potentially related to KCOT progression were selected and their expression levels were confirmed by quantitative PCR, immunohistochemistry and enzyme-linked immunosorbent assay. Expression of lysyl oxidase-like 4 (LOXL4), the only candidate gene that encodes a secreted protein, was enhanced at both the mRNA and protein levels in KCOT stromal tissues and primary KCOT stromal fibroblasts compared to control tissues and primary fibroblasts (P〈0.05). In vitro, high expression of LOXL4 could enhance proliferation and migration of the human umbilical vein endothelial cells (HUVECs). There was a significant, positive correlation between LOXL4 protein expression and MVD in stroma of KCOT and control tissues (r=0.882). These data suggest that abnormal expression of LOXL4 of KCOT may enhance angiogenesis in KCOT, which may help to promote the locally aggressive biological behavior of KCOT.
文摘Objective:To observe the effects of different anesthesia depths on the level of plasma cortisol during operation and the score of postoperative MMSE in elderly patients underwent radical gastrectomy.Methods: A total of eighty seven elderly patients undergoing radical gastrectomy were enrolled in this study. Continuous infusion of propofol and remifentanil for anesthesia and maintenance of propofol were divided into two groups: BIS-I (BIS: 45-55) and BIS-II (BIS: 55-65) by adusting the doses of propofol. The data were recorded as following, thewhole dosage of propofol, the time of extubation and the score of VAS. The level of plasma cortisol was finished at the time of incision and tracheal intubation. MMSE scores were recorded on the day before operation and follow-up at 3 and 7 d postoperatively. Record the patient's operation to discharge time, postoperative complications.Results: The concentration of cortisol in BIS-I group was higher than that in BIS-II group. There was no significant difference in extubation time, extubation VAS score and MMSE score after operation.Conclusion:There were no significant differences in the BIS values between 45 and 65 for the elderly patients undergoing upper abdominal surgery, and there were no significant differences in the patients' postoperative stress, wake, extubation, early postoperative pain scores and postoperative cognitive function. BIS values in 45-55 compared to 55-65 patients with low plasma cortisol levels may be associated with better control of stress levels, to maintain the BIS value between 45-55 anesthesia depths is more reasonable.
基金financially supported by the National Aerospace Science Foundation of China(No.2012ZE51058)。
文摘Smooth and compact nickel layers were successfully prepared in the ionic liquid of 1-butyl-3-methylimidazolium tetrafluoroborate(BMIMBF4)by constant current electrodeposition method.The effects of temperature,additive content,current density,and deposition time on the performance of the nickel layers were systematically analyzed.Scanning electron microscopy(SEM)and X-ray diffraction(XRD)were used to analyze the surface morphology and composition of the nickel layers.Meanwhile,the tensile strengths of the nickel layers were tested by universal tensile testing machine.The results show that different process conditions have a great effect on morphology and performance of the electrodeposited nickel layer.The optimization of process parameters is as follows:BMIMBF4 to ethylene glycol(EG)volume ratio of 2:1,deposition temperature of 120℃,and current density of 1.2 mA·cm^(-2).Current density has a greater influence on the tensile strength of the nickel layer,and the maximum value of tensile strength is 1275 MPa.
基金financially supported by the Aeronautical Science Foundation of China(No.2012ZE51058)。
文摘Molybdenum is a kind of refractory metal and can hardly be deposited in aqueous solution since it is more likely to form molybdenum oxide rather than elementary Mo,but molten salts and ionic liquid become alternative to obtain Mo layer.This paper shows an available process to obtain metallic state molybdenum deposit from ionic liquid.Mo layer was electrodeposited under constant current in ionic liquid 1-Butyl-3-methylimidazolium tetrafluoroborate(BMIMBF_4).Micromorphology of the electrodeposited Mo layer was observed by scanning electron microscope(SEM),energy dispersive spectroscopy(EDS)and X-ray photoelectron spectroscopy(XPS).Results show that elementary Mo layer was electrodeposited under constant current in ionic liquid BMIMBF4.Properties of the Mo layer are affected significantly by additive ethylene glycol(EG),deposition temperature and current density.By parallel tests taking into consideration of different technical factors,optimized process parameters are obtained as volume ratio of 2:1 in BMIMBF4+EG system,deposition temperature of 150℃and current density of 0.75 mA·cm^(-2).
基金financially supported by the Aviation Science Foundation,China (No.2012ZE51058)。
文摘In order to prepare electroformed nickel with excellent properties,the effect of different waveforms on microstructure and mechanical properties was investigated by optical microscopy(OM),X-ray diffraction(XRD) and tensile test,respectively.The results show that the samples are composed of micron columnar grains with high-density nanoscale coherent twin boundaries which are parallel to the growth plane.The tensile strength and elongation display opposite tendency with the increase in current density when direct current(DC) electroforming was applied.However,under the condition of pulse current(unidirectional,bidirectional) electroforming,the change regulation of tensile strength and elongation is close while cathode(peak,positive peak) current density increases.XRD results show that electroformed nickel layers with high purity are all fcc crystal configuration under different conditions of the current waveform.The nickel layers formed under bidirectional pulse current behave superior global performance with tensile strength of 692.0 MPa and elongation of 27.6 %.
基金supported by the National Natural Science Foundation of China(Nos.31330075,31402089,31301988,31572420,31272450,and 31272451)the National Key Technology R&D Program of China(No.2016YFD0500504)+2 种基金the Changsha Lvye Biotechnology Limited Company Academician Expert Workstationthe Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additivesthe Animal Intestinal Health Hunan New Wellful Co.,Ltd.,Academician Workstation,Changsha,China
基金Project supported by the National Basic Research Program(973)of China(No.2013CB127301)the Natural Science Foundation of Hunan Province,China(No.12JJ2014)the National Natural Science Foundation of China(No.31402088)
文摘目的:验证日粮中粗蛋白质浓度对断奶仔猪氨基酸转运载体的影响效应。创新点:从营养物质感应体的角度分析采用低氮日粮的可行性,并探索营养限制对营养转运体的影响及它们间的相互作用。方法:十八头初始体重9.57公斤的断奶仔猪被随机分为三组(每组6个重复),分别饲喂含有14%、17%和20%的粗蛋白日粮45天。按照理想蛋白质模型,日粮分别添加赖氨酸、蛋氨酸+半胱氨酸、苏氨酸和色氨酸来满足断奶仔猪的需要,整个试验期自由采食。试验结束后,屠宰仔猪并采集小肠样品。与14%粗蛋白组相比,空肠中ASCT2、4F2hc和ATB0 m RNA表达在17%和20%粗蛋白水平组分别上升23.00%、12.00%、6.00%和48.00%、47.00%、56.00%。结论:结果表明,14%粗蛋白水平组外源添加合成氨基酸并不能促进氨基酸转运载体增加氨基酸的吸收来满足断奶仔猪的生长性能。然而,17%粗蛋白水平组可以减少氮排放到环境中,同时又能满足此阶段断奶仔猪的生长发育。因此,17%粗蛋白水平组对这个阶段断奶仔猪最合适。