Detection of circulating tumor cells(CTCs)plays an important role in early diagnosis of cancer and personalized therapy.However,isolated CTCs,especially those captured by positive sorting methods,are difficult to cult...Detection of circulating tumor cells(CTCs)plays an important role in early diagnosis of cancer and personalized therapy.However,isolated CTCs,especially those captured by positive sorting methods,are difficult to culture in subsequent assays because the cells have to be labeled or attached to a substrate for separation.In this study,a negative sorting method has been developed for isolation of CTCs through a microfluidic platform integrated with streptavidin-functionalized electro-spun polylactic-co-glycolic acid nanofibers.Through the specific biotin-streptavidin interaction,the device is able to sort out biotinylated anti-CD45 antibody-labeled white blood cells(WBCs)and enrich A549 human cancer cells from the blood or CTCs from patient suffering non-small cell lung cancer.We demonstrate that the WBC capture efficiency is as high as 97.0%,and the recovery rate of cancer cells reaches up to 97.5%.CTCs are enumerated from blood samples of patients suf-fering lung carcinoma.The number of CTCs increased with the progression of NCCN TNM stages and showed statistically significant difference between stage I and later stages.These results suggest that the integrated negative sorting device is promising to be used for diagnosis of cancer.展开更多
基金the National Natural Science Foundation of China(21405012 and 81761148028)the Science and Technology Commission of Shanghai Municipality(19XD1400100 and 20DZ2254900)the Shanghai Education Commission through the Shanghai Leading Talents Program,and the 111 Project(BP0719035).
文摘Detection of circulating tumor cells(CTCs)plays an important role in early diagnosis of cancer and personalized therapy.However,isolated CTCs,especially those captured by positive sorting methods,are difficult to culture in subsequent assays because the cells have to be labeled or attached to a substrate for separation.In this study,a negative sorting method has been developed for isolation of CTCs through a microfluidic platform integrated with streptavidin-functionalized electro-spun polylactic-co-glycolic acid nanofibers.Through the specific biotin-streptavidin interaction,the device is able to sort out biotinylated anti-CD45 antibody-labeled white blood cells(WBCs)and enrich A549 human cancer cells from the blood or CTCs from patient suffering non-small cell lung cancer.We demonstrate that the WBC capture efficiency is as high as 97.0%,and the recovery rate of cancer cells reaches up to 97.5%.CTCs are enumerated from blood samples of patients suf-fering lung carcinoma.The number of CTCs increased with the progression of NCCN TNM stages and showed statistically significant difference between stage I and later stages.These results suggest that the integrated negative sorting device is promising to be used for diagnosis of cancer.
