Suppression of tumorigenesis by human mesenchymal stem cells in a hepatoma model

人的间充质的干细胞(hMSCs ) 装家到肿瘤地点并且禁止肿瘤细胞的生长。很少对内在的分子的机制被知道连接 hMSCs 到肿瘤房间的指向的抑制。在这研究，我们从 hMSCs 用一个动物移植模型，一个合作文化系统和调节媒介在二根人的 hepatoma 房间线(H7402 和 HepG2 ) 上调查了 hMSCs 的效果。当 SCID 老鼠与 H7402 细胞和 Z3 hMSCs 的一个相等的数字被注射时，动物移植研究证明肿瘤形成的潜伏的时间被延长并且肿瘤尺寸更小。当 co 有教养时与 Z3 房间， H7402 细胞增殖减少了，增加的 apoptosis，和 Bcl-2 的表示， c-Myc，原子抗原(PCNA ) 和 survivin 低是的增殖的房间调整了。在有调节媒介的处理源于 Z3 hMSC 文化以后， H4702 房间出现了减少的形成殖民地的能力和减少的增长。Immunoblot 分析证明 beta-catenin， Bcl-2， c-Myc， PCNA 和 survivin 表示是在 H7402 和 HepG2 房间调整的 down。总起来说，我们的调查结果证明 hMSCs 禁止 H7402 和 HepG2 人的肝癌症房间线的恶意的显型，它包括增长，形成殖民地的能力和 oncogene 表示试管内和体内。而且，我们的研究提供表明小径的 Wnt 可以在调停 hMSC 的指向和肿瘤房间抑制有一个角色的证据。

Convolutional Multi-Head Self-Attention on Memory for Aspect Sentiment Classification

This paper presents a method for aspect based sentiment classification tasks, named convolutional multi-head self-attention memory network(CMA-Mem Net). This is an improved model based on memory networks, and makes it possible to extract more rich and complex semantic information from sequences and aspects. In order to fix the memory network’s inability to capture context-related information on a word-level,we propose utilizing convolution to capture n-gram grammatical information. We use multi-head self-attention to make up for the problem where the memory network ignores the semantic information of the sequence itself. Meanwhile, unlike most recurrent neural network(RNN) long short term memory(LSTM), gated recurrent unit(GRU) models, we retain the parallelism of the network. We experiment on the open datasets Sem Eval-2014 Task 4 and Sem Eval-2016 Task 6. Compared with some popular baseline methods, our model performs excellently.

Tensile Behavior of Strain Hardening Cementitious Composites (SHCC) Containing Reactive Recycled Powder from Various C&D Waste

This work investigates the feasibility of utilizing reactive recycled powder(RP)from construction and demolition(C&D)waste as supplementary cementitious material(SCM)to achieve a ductile strain hardening cementitious composites(SHCC).The recycled mortar powder(RMP)from mortar waste,recycled concrete powder(RCP)from concrete waste and recycled brick powder(RBP)from clay brick waste were first prepared,and the micro-properties and tensile behavior of SHCC containing various types and replacement ratios of RPs were determined.The incorporated RP promotes pozzolanic and filler effects,while the hydration products in cementitious materials decrease with RP incorporation;therefore,the incorporated RP decreases the compressive strength of SHCC.Attributed to the reduction in the matrix strength,the incorporated RP increases the crack-bridging extent and ductility of SHCC;the irregular micro-structure and high reactivity of RP also help the strain-hardening performance of the prepared SHCC.In addition,the strainhardening performance of SHCC containing RMP and RBP is surperior to that of SHCC with RCP and is slightly lower than that of SHCC with fly ash(FA);for instance,the ultimate strain of SHCC containing 54%FA,RMP,RCP and RBP is 3.67%,3.61%,2.52%and 3.53%,respectively.In addition,the strain-hardening behavior of an SHCC doubled mix with FA and RMP or RBP has a similar ultimate strain and a higher ultimate stress than SHCC containing only FA.

Sentence-Level Paraphrasing for Machine Translation System Combination

In this paper, we propose to enhance machine translation system combination (MTSC) with a sentence-level paraphrasing model trained by a neural network. This work extends the number of candidates in MTSC by paraphrasing the whole original MT translation sentences. First we train a neural paraphrasing model of Encoder-Decoder, and leverage the model to paraphrase the MT system outputs to generate synonymous candidates in the semantic space. Then we merge all of them into a single improved translation by a state-of-the-art system combination approach (MEMT) adding some new paraphrasing features. Our experimental results show a significant improvement of 0.28 BLEU points on the WMT2011 test data and 0.41 BLEU points without considering the out-of-vocabulary (OOV) words for the sentence-level paraphrasing model.

Preparation of nanocrystalline Na_2ZrO_3 for high-temperature CO_2 acceptors:chemistry and mechanism

Nanocrystalline Na2ZrO3 was demonstrated as a promising acceptor for CO2 capture at elevated temperatures. The mechanism of nanocrystalline Na2ZrO3 formation from the soft-chemistry route is elucidated by varying precursors, preparation methods, and calciantion temperatures, combining detailed characterizations by X-ray diffraction （XRD） and scanning electron microscope （SEM） at different steps in the process. The results revealed that the drying method such as spraying drying and simple evaporation-drying did not influence the final product prop- erties. However both Na and Zr precursors had remarkable influences on the Na2ZrO3 formation. The solid reaction of Na intermediate and nanocrystalline ZrO2 in the calcination was identified as the key step for the Na2ZrO3 formation, where the formation of molten phase Na intermediate was found to be crucial to facilitate the solid reaction. We provided principles for rational design of the chemistry for the Na2ZrO3 formation where the formation of Na intermediate with low melting points is essential. Pure nanocrystalline Na2ZrO3 can be synthesized from a mixture containing sodium nitrate and zirconoxy citrate via the formation of NaNO3 with low melting point. However, it is not possible to form pure nanocrystalline Na2ZrO3 at relatively low temperatures from the mixtures of NaAc/ZrO（NO3）2 or NaCA/ZrOC12 due to the formation of Na2CO3 and NaC1 with high melting points.

Identification of differential mRNA and lncRNA expression in AcMNPV-infected Sf9 cells

Sf9Sf9 are the ovarian cells of Spodoptera frugiperda that is the host of Autographa californica multiple nucleopolyhedrovirus(AcMNPV),and hence can serve as an effective test vehicle to understand the AcMNPV infection mechanism.In this study,through high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection,3463 pieces of time-series differentially expressed RNA(1,200 mRNA and 2,263 lncRNA)are identified and justified by experimental verification of randomly selected samples from them,proving the validity of the bioinformatical analysis on this topic.Functional enrichment analysis and target prediction are performed on those differentially expressed RNA,from which the major functional enrichment distribution of those differentially expressed mRNA is derived.It has been found that the differential genes are mainly in the cellular anatomical entity and intracellular in terms of the cellular component,and in the binding and catalytic activity in terms of the molecular function.Also,the differential mRNA are mainly concentrated in global and overview maps,signal transduction,infectious diseases,and viral,etc.Moreover,those mRNA targeted by lncRNA are predicted.The correlation between those differentially expressed lncRNA and mRNA indicates that lncRNA is very likely playing an important role in the interaction between virus and host.Aided by an advanced co-expression analysis approach,the“hub”RNA is also identified.The study in this work pave the way for further analyzing and understanding how AcMNPV escapes from the host’s immunity,manipulates the host to realize the selfmultiplication,and realizes the timely conversion between its two particle forms,laying the foundation for uncovering the host’s immune response process.

microR-1294-5p inhibits glycolytic metabolism of non-small cell lung cancer cells via targeting TMPRSS11B

Non-smallcell lung cancer(NSCLC)cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust cell growth and resist cell death.MicroRNAs(miRNAs)have been known to play pivotal roles in NSCLC development partly through mediating glycolysis.However,only a few miRNAs have been experimentally confirmed as critical regulators of glycolysis in NSCLC.TCGA datasets were analyzed to screen for differentially expressed miRNAs between NSCLC and normal tissues.The function of miR-1294-5p was determined in NSCLC cells by cell proliferation,glucose uptake,lactate release,and Extracellular Acidification Rate(ECAR)assays.The target of miR-1294-5p was predicted by TargetScan and miRDB,which was further validated by flow cytometry analysis,RT-qPCR,western blotting,a dual-luciferase reporter assay,and RNA immunoprecipitation(RIP)assay.In the present study,it was found that miR-1294-5p was a significantly downregulated miRNA in lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC).The overexpression of miR-1294-5p inhibited glycolysis,lactate export,ECAR,and cell proliferation in NSCLC cells.Analysis with bioinformatic tools,Western Blotting,RT-qPCR,flow cytometry analysis,dual-luciferase reporter assay,and RIP assay showed that miR-1294-5p directly bound to complementary sites in the 3’-Untranslated Region(UTR)of TMPRSS11B resulted in downregulation of TMPRSS11B expression.In addition,transfection of recombinant TMPRSS11B rescued the functions of miR-1294-5p on glycolysis and proliferation of NSCLC cells.The findings provided novel insights for understanding the regulation of glycolytic metabolism in NSCLC.

A Context-Aware Model Using Distributed Representations for Chinese Zero Pronoun Resolution

Previous approaches to Chinese zero pronoun resolution mainly use syntactic information and probabilistic methods, but the context information is ignored. To make full use of the context and semantic information, we build a context-aware model. We propose a key words extraction strategy and design a classification model by using distributed representations as context feature. To our best knowledge, this is the first work using distributed representations in Chinese zero pronoun resolution. Experimental results show that our approach achieves a better performance than previous supervised methods.

新冠病毒N蛋白与G3BP发生相分离从而促进应激颗粒解组装和病毒产生

新型冠状病毒SARS-CoV-2的爆发导致全世界数以百万计人员的感染和死亡,有效的治疗和干预措施对疫情的控制至关重要.然而到目前为止,新冠病毒逃过宿主抗病毒反应的机制尚未明确.病毒感染宿主细胞时,宿主能够迅速启动细胞的压力应答机制,终止细胞内的蛋白翻译,形成应激颗粒.本研究发现新型冠状病毒核衣壳蛋白(N蛋白)—一种在病毒组装过程非常关键的RNA结合蛋白,与宿主的G3BP蛋白(应激颗粒组装过程中关键的RNA结合蛋白)相互作用,并促进应激颗粒的解组装.N蛋白通过与G3BP发生相分离从而渗入应激颗粒中,阻断了G3BP与其他应激颗粒内蛋白的相互作用,最终破坏了应激颗粒的组装.这一N蛋白介导的相分离过程最终会促进病毒逃过宿主的抗病毒反应,从而有利于病毒的产生.

Effect of Ermiao Fang with Xixin(Herba Asari Mandshurici) on bone marrow stem cell directional homing to a focal zone in an osteoarthritis rat model

OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone.

Recent advances in the catalytic conversion of CO_2 to value added compunds

CO2 is a major greenhouse gas,and it can also be used as a chemical feedstock for synthesis of chemicals and fuels by passing the petrochemical source.Herein,we present the recent progress of our research work in the catalytic conversion of CO2 to chemicals,with particular attention paid to catalytic reactivity and reaction mechanism.We also give the recommendations regarding the challenges and potential directions of the future research in this field.

Super broadband absorbing hierarchical CoFe alloy/porous carbon@carbon nanotubes nanocomposites derived from metal-organic frameworks

The exploitation of electromagnetic wave absorption(EMA) materials has attracted ever-increasing attention,not only because electromagnetic wave(EMW) originated from overuse of electronic products has threatened human' health seriously,but also because EMA materials can effectively protect radar stealth from being detected.However,it is still a challenge to obtain broadband and efficient EMA materials to satisfy practical applications.In this work,we developed a series of hierarchical CoFe alloy/porous carbon@carbon nanotubes(CoFe/PC@CNTs) nanocomposites through revising the proportion of Fe^(2+) in the metal-organic frameworks(MOFs)(CoFe-ZIF) precursor and one-step simple pyrolysis process.The cross deposition between CoFe alloy,carbon nanotubes(CNTs),and porous carbon(PC) formed a double conductive network,favoring for achieving excellent EMA property.Surprisingly,when the filler mass ratio is only 10 wt%,the optimized CoFe/PC@CNTs nanocomposites display the best EMA capability,whose minimum reflection loss(RL_(min)) value is-68.94 dB at 13.69 GHz and the effective absorption band(EAB,<-10 dB) reaches up 9.14 GHz with a matching thickness of 2.63 mm.In addition,the largest EAB can achieve up to 11 GHz(3.35-14.35 GHz) during all matching thicknesses.The splendid EMA performance benefits the favorable dielectric loss provided by the double conductive network,the good magnetic loss benefited from the evenly distributed CoFe alloy,the excellent impedance matching,rich transmission paths,and multiple polarization.Therefore,such EMA materials with super broadband absorbing provide desirable candidates for lightweight and high-efficient microwave absorbers.

Differentiated super-enhancers in lung cancer cells

Super-enhancers(SEs) are regulatory elements with enriched accumulation of key transcription factors.Few studies were done investigating SEs in lung cancers.Here we analyzed epigenetic profiling data to identify SEs in lung cancer cell lines.Enhancers were classified as SEs and typical enhancers(TEs).Most of the TEs were overlapped between normal cell and cancer cells.A great portion of SEs were differentiated comparing these cells.Analysis of GO terms associated with SEs revealed SE remodeling(lost on some sites while gain on others) between normal and lung cancer cells.By comparing the average number of SEs in each GO term in cancer cells with the number in control cells,surprisingly,no GO terms with significantly increased SE number in cancer condition were observed.On the contrary,in aspects such as "cell-cell adhesion","receptor activity" and"negative regulation of canonical Wnt signaling pathway",the related SEs were significantly reduced in cancer cells.These findings suggest that in lung cancer,cells may not gain decisive gene expression in the related aspect,instead,they may have lost control of the fateful genes.Taken together,our work with the usability of omics data identified SEs in lung cancer cells and further showed cancer-specific features of SE-related terms.