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基于异肽键连接的分子粘合剂研究进展 被引量:1
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作者 高德英 曹佳雯 +3 位作者 孙小宝 周可鑫 张铁涛 王谦 《生物工程学报》 CAS CSCD 北大核心 2019年第4期607-615,共9页
异肽键分子粘合剂是多肽链中的赖氨酸(Lys)残基和天冬酰胺或天冬氨酸(Asn/Asp)残基侧链自发结合形成的不可逆共价键,具有良好的特异性和稳定性。该反应可在极短的时间完成,且不需要特定的理化环境和催化剂。文中结合近年来国内外学者对... 异肽键分子粘合剂是多肽链中的赖氨酸(Lys)残基和天冬酰胺或天冬氨酸(Asn/Asp)残基侧链自发结合形成的不可逆共价键,具有良好的特异性和稳定性。该反应可在极短的时间完成,且不需要特定的理化环境和催化剂。文中结合近年来国内外学者对异肽键分子粘合剂的研究进展,综述了异肽键分子粘合剂的来源、类型、形成机制及其介导的分子环化和蛋白拓扑结构,并讨论了其在合成疫苗、水凝胶和细菌纳米生物反应器等领域的应用前景。 展开更多
关键词 异肽键 分子环化 机制 SpyTag/SpyCatcher 应用
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Effects of dietary protein level on nutrients digestibility and reproductive performance of female mink(Neovison vison)during gestation 被引量:4
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作者 Qingkui Jiang Guangyu Li +5 位作者 tietao zhang Haihua zhang Xiuhua Gao Xiumei Xing Jiaping Zhao Fuhe Yang 《Animal Nutrition》 SCIE 2015年第2期65-69,共5页
The objective of this study was to determine whether nutrient digestibility and reproductive performance of pregnant mink(Neovison vison) were affected by different dietary protein levels. One hundred and twenty femal... The objective of this study was to determine whether nutrient digestibility and reproductive performance of pregnant mink(Neovison vison) were affected by different dietary protein levels. One hundred and twenty female mink were randomly assigned to four groups, receiving diets of fresh material with different protein levels. The dietary protein levels,expressed as percentage of dry matter(DM),were 32,36, 40 and 44% respectively. These values corresponded to average 320, 360, 400 and 440 g protein/kg DM, respectively. Results were as follows. All of crude protein digestibility, nitrogen(N) intake, N retention increased along with dietary protein level increasing. Low protein level(32%) significantly reduced the above indicators(P < 0.05). DM digestibility and ether extract digestibility were not affected by dietary protein level. Results of mated females, barren females, kids per litter, live born kids per mated female, birth survival rate, and birth weight showed that mink achieved optimal reproductive performance when dietary protein level was 36%. In conclusion, dietary protein was anticipated to significantly influence some nutrients' utilization. Adopting the appropriate dietary protein level allow better reproduction performance. The most preferable reproductive performance was achieved when diet contained 275.5 g digestible protein per kg DM for female mink in gestation. 展开更多
关键词 Female mink Protein level GESTATION Reproductive performance
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Effects of dry dietary protein on digestibility, nitrogen-balance and growth performance of young male mink 被引量:2
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作者 tietao zhang Haihua zhang +6 位作者 Xuezhuang Wu Qiang Guo Zhi Liu Wang Qian Xiuhua Gao Fuhe Yang Guangyu Li 《Animal Nutrition》 SCIE 2015年第2期60-64,共5页
The experiment was to study the nutrient digestibility and metabolism performance of male minks,which were fed different protein level diets during growth period. Effects of protein quantity on growth and development ... The experiment was to study the nutrient digestibility and metabolism performance of male minks,which were fed different protein level diets during growth period. Effects of protein quantity on growth and development of minks and feed conversion ratio(FCR) were also investigated. Sixty healthy male minks of 45 d were randomly allocated into six groups with ten replicates, which was one sable for each replicate. The minks in six groups were fed diets in which protein levels were 28, 30,32, 34, 36 and 38%,respectively. The six groups were denoted as P28, P30, P32, P34, P36 and P38. After 2 wk, all minks were weighed, average daily gains(ADG) were calculated, and the digestibility values of nutrients were determined. The results indicated that digestibility of calcium, nitrogen of fence, nitrogen deposition, net protein utilization(NPU), and biological value of protein(BV) were similar(P > 0.05), however, nitrogen intake greatly varied among groups(P < 0.01). Compared with group P28, groups of P34, P36 and P38 showed significant difference(P < 0.01) in ADG and FCR. In conclusion, it was recommended that adding34% protein to mink diet would optimize production parameters including ADG, digestibility of nutrition,and FCR, and negative result was observed when diet protein was lower than 28%. 展开更多
关键词 MINK Protein DIGESTIBILITY Growth performance
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Effects of dietary methionine supplementation on growth performance of cubs,nutrient digestibility, nitrogen metabolism and serum biochemical indicators of female blue foxes(Alopex lagopus) 被引量:1
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作者 Jungang Guo Xuezhuang Wu +4 位作者 tietao zhang Zhi Liu Hu Cui Fuhe Yang Xiuhua Gao 《Animal Nutrition》 SCIE 2015年第4期378-382,共5页
The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of femal... The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of female blue foxes. One hundred primiparous female blue foxes that were similar in breeding date, pedigree, age, and weight were selected for the trial. The foxes were randomly assigned to four groups(n = 25 each group) and fed diets supplemented with Met at 2(Met2), 4(Met4), 6(Met6)and 8 g/kg(Met8), respectively, for 40 days. Our data showed that body weights at 20 and 40 d were significantly higher in the Met4 group than in the Met2 group(P < 0.05). The Met4 group also had the highest apparent digestibility of dry matter and crude protein compared with either the Met2, Met6, or Met8 group(P< 0.05). The serum Met and isoleucine(Ile) concentrations were significantly higher in the Met4 group than in the Met6 or Met8 group(P < 0.05). In summary, these data indicate that supplementary Met improves growth performance of cubs likely due to increased crude protein and dry matter and increased nitrogen retention of female blue foxes. The optimal amount of Met supplementation is10 g/kg basal diet. 展开更多
关键词 Blue fox METHIONINE Growth performance Nutrient digestibility Nitrogen metabolism Serum biochemical indicators
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