Advancements in microscale electrode technology have revolutionized the field of neuroscience and clinicalapplications by offering high temporal and spatial resolution of recording and stimulation. Flexible neural pro...Advancements in microscale electrode technology have revolutionized the field of neuroscience and clinicalapplications by offering high temporal and spatial resolution of recording and stimulation. Flexible neural probes, withtheir mechanical compliance to brain tissue, have been shown to be superior to rigid devices in terms of stability andlongevity in chronic recordings. Shuttle devices are commonly used to assist flexible probe implantation;however, theprotective membrane of the brain still makes penetration difficult. Hidden damage to brain vessels duringimplantation is a significant risk. Inspired by the anatomy of the mosquito mouthparts, we present a biomimeticneuroprobe system that integrates high-sensitivity sensors with a high-fidelity multichannel flexible electrode array.This customizable system achieves distributed and minimally invasive implantation across brain regions. Mostimportantly, the system’s nonvisual monitoring capability provides an early warning detection for intracranial softtissues, such as vessels, reducing the potential for injury during implantation. The neural probe system demonstratesexceptional sensitivity and adaptability to environmental stimuli, as well as outstanding performance in postoperativeand chronic recordings. These findings suggest that our biomimetic neural-probe device offers promising potential forfuture applications in neuroscience and brain-machine interfaces.展开更多
The combination of optogenetics and electrophysiological recording enables high-precision bidirectional interactions between neural interfaces and neural circuits,which provides a promising approach for the study of p...The combination of optogenetics and electrophysiological recording enables high-precision bidirectional interactions between neural interfaces and neural circuits,which provides a promising approach for the study of progressive neurophysiological phenomena.Opto-electrophysiological neural probes with sufficient flexibility and biocompatibility are desirable to match the low mechanical stiffness of brain tissue for chronic reliable performance.However,lack of rigidity poses challenges for the accurate implantation of flexible neural probes with less invasiveness.Herein,we report a hybrid probe(Silk-Optrode)consisting of a silk protein optical fiber and multiple flexible microelectrode arrays.The Silk-Optrode can be accurately inserted into the brain and perform synchronized optogenetic stimulation and multichannel recording in freely behaving animals.Silk plays an important role due to its high transparency,excellent biocompatibility,and mechanical controllability.Through the hydration of the silk optical fiber,the Silk-Optrode probe enables itself to actively adapt to the environment after implantation and reduce its own mechanical stiffness to implant into the brain with high fidelity while maintaining mechanical compliance with the surrounding tissue.The probes with 128 recording channels can detect high-yield well-isolated single units while performing intracranial light stimulation with low optical losses,surpassing previous work of a similar type.Two months of post-surgery results suggested that as-reported Silk-Optrode probes exhibit better implant-neural interfaces with less immunoreactive glial responses and tissue lesions.展开更多
In implantable electrophysiological recording systems,the headstage typically comprises neural probes that interface with brain tissue and integrated circuit chips for signal processing.While advancements in MEMS and ...In implantable electrophysiological recording systems,the headstage typically comprises neural probes that interface with brain tissue and integrated circuit chips for signal processing.While advancements in MEMS and CMOS technology have significantly improved these components,their interconnection still relies on conventional printed circuit boards and sophisticated adapters.This conventional approach adds considerable weight and volume to the package,especially for high channel count systems.To address this issue,we developed a through-polymer via(TPV)method inspired by the through-silicon via(TSV)technique in advanced three-dimensional packaging.This innovation enables the vertical integration of flexible probes,amplifier chips,and PCBs,realizing a flexible,lightweight,and integrated device(FLID).The total weight of the FLIDis only 25%that of its conventional counterparts relying on adapters,which significantly increased the activity levels of animals wearing the FLIDs to nearly match the levels of control animals without implants.Furthermore,by incorporating a platinum-iridium alloy as the top layer material for electrical contact,the FLID realizes exceptional electrical performance,enabling in vivo measurements of both local field potentials and individual neuron action potentials.These findings showcase the potential of FLIDs in scaling up implantable neural recording systems and mark a significant advancement in the field of neurotechnology.展开更多
基金This work was partially supported by the National Key R&D Program of China(Grant Nos.2019YFA0905200,2021ZD0201600,2021YFC2501500,2021YFF1200700,2022ZD0209300,2022ZD0212300,2022YFF0706500)National Natural Science Foundation of China(Grant No.61974154)+8 种基金Key Research Program of Frontier Sciences,CAS(Grant No.ZDBS-LYJSC024)Shanghai Pilot Program for Basic Research—Chinese Academy of Science,Shanghai Branch(Grant No.JCYJ-SHFY-2022-01)Shanghai Municipal Science and Technology Major Project(Grant No.2021SHZDZX)CAS Pioneer Hundred Talents Program,Shanghai Pujiang Program(Grant Nos.19PJ1410900,21PJ1415100)the Science and Technology Commission Foundation of Shanghai(No.21JM0010200)Shanghai Rising-Star Program(Grant No.22QA1410900)the Innovative Research Team of High-level Local Universities in Shanghai,the Jiangxi Province 03 Special Project and 5G Project(Grant No.20212ABC03W07)Fund for Central Government in Guidance of Local Science and Technology Development(Grant No.20201ZDE04013)Special Fund for Science and Technology Innovation Strategy of Guangdong Province(Grant Nos.2021B0909060002,2021B0909050004).
文摘Advancements in microscale electrode technology have revolutionized the field of neuroscience and clinicalapplications by offering high temporal and spatial resolution of recording and stimulation. Flexible neural probes, withtheir mechanical compliance to brain tissue, have been shown to be superior to rigid devices in terms of stability andlongevity in chronic recordings. Shuttle devices are commonly used to assist flexible probe implantation;however, theprotective membrane of the brain still makes penetration difficult. Hidden damage to brain vessels duringimplantation is a significant risk. Inspired by the anatomy of the mosquito mouthparts, we present a biomimeticneuroprobe system that integrates high-sensitivity sensors with a high-fidelity multichannel flexible electrode array.This customizable system achieves distributed and minimally invasive implantation across brain regions. Mostimportantly, the system’s nonvisual monitoring capability provides an early warning detection for intracranial softtissues, such as vessels, reducing the potential for injury during implantation. The neural probe system demonstratesexceptional sensitivity and adaptability to environmental stimuli, as well as outstanding performance in postoperativeand chronic recordings. These findings suggest that our biomimetic neural-probe device offers promising potential forfuture applications in neuroscience and brain-machine interfaces.
基金partially supported by the National Key R&D Program of China(Grant Nos.2019YFA0905200,2021ZD0201600,2021YFC2501500,2021YFF1200700,2022ZD0209300,2022ZD0212300)National Natural Science Foundation of China(Grant No.61974154)+8 种基金Key Research Program of Frontier Sciences,CAS(Grant No.ZDBSLY-JSC024)Shanghai Pilot Program for Basic Research—Chinese Academy of Science,Shanghai Branch(Grant No.JCYJ-SHFY-2022-01)Shanghai Municipal Science and Technology Major Project(Grant No.2021SHZDZX)CAS Pioneer Hundred Talents Program,Shanghai Pujiang Program(Grant Nos.19PJ1410900,21PJ1415100)the Science and Technology Commission Foundation of Shanghai(No.21JM0010200)Shanghai Rising-Star Program(Grant No.22QA1410900)the Innovative Research Team of High-level Local Universities in Shanghai,the Jiangxi Province 03 Special Project and 5G Project(Grant No.20212ABC03W07)Fund for Central Government in Guidance of Local Science and Technology Development(Grant No.20201ZDE04013)Special Fund for Science and Technology Innovation Strategy of Guangdong Province(Grant Nos.2021B0909060002,2021B0909050004)。
文摘The combination of optogenetics and electrophysiological recording enables high-precision bidirectional interactions between neural interfaces and neural circuits,which provides a promising approach for the study of progressive neurophysiological phenomena.Opto-electrophysiological neural probes with sufficient flexibility and biocompatibility are desirable to match the low mechanical stiffness of brain tissue for chronic reliable performance.However,lack of rigidity poses challenges for the accurate implantation of flexible neural probes with less invasiveness.Herein,we report a hybrid probe(Silk-Optrode)consisting of a silk protein optical fiber and multiple flexible microelectrode arrays.The Silk-Optrode can be accurately inserted into the brain and perform synchronized optogenetic stimulation and multichannel recording in freely behaving animals.Silk plays an important role due to its high transparency,excellent biocompatibility,and mechanical controllability.Through the hydration of the silk optical fiber,the Silk-Optrode probe enables itself to actively adapt to the environment after implantation and reduce its own mechanical stiffness to implant into the brain with high fidelity while maintaining mechanical compliance with the surrounding tissue.The probes with 128 recording channels can detect high-yield well-isolated single units while performing intracranial light stimulation with low optical losses,surpassing previous work of a similar type.Two months of post-surgery results suggested that as-reported Silk-Optrode probes exhibit better implant-neural interfaces with less immunoreactive glial responses and tissue lesions.
基金supported by the National Key R&D Program of China(Grant Nos.2021ZD0201600,2022YFF0706504,2022ZD0209300,2019YFA0905200,2021YFC2501500,2021YFF1200700,2022ZD0212300)the National Natural Science Foundation of China(Grant No.61974154)+11 种基金the Key Research Program of Frontier Sciences,CAS(Grant No.ZDBS-LY-JSC024)the Shanghai Pilot Program for Basic Research-Chinese Academy of Science,the Shanghai Branch(Grant No.JCYJ-SHFY-2022-01 and JCYJ-SHFY-2022-0xx)the Shanghai Municipal Science and Technology Major Project(Grant No.2021SHZDZX)the CAS Pioneer Hundred Talents Program,the Shanghai Pujiang Program(Grant Nos.21PJ1415100,19PJ1410900)the Science and Technology Commission Foundation of Shanghai(Nos.21JM0010200 and 21142200300)the Shanghai Rising-Star Program(Grant No.22QA1410900)Shanghai Sailing Program(No.22YF1454700)the Innovative Research Team of High-level Local Universities in Shanghai,the Jiangxi Province 03 Special Project and 5 G Project(Grant No.20212ABC03W07)Fund for Central Government in Guidance of Local Science and Technology Development(Grant No.20201ZDE04013)Special Fund for Science and Technology Innovation Strategy of Guangdong Province(Grant Nos.2021B0909060002,2021B0909050004)the Young Scientists Fund of the National Natural Science Foundation of China(Grant No.62305368)the Youth Innovation Promotion Association for Excellent Members,CAS.
文摘In implantable electrophysiological recording systems,the headstage typically comprises neural probes that interface with brain tissue and integrated circuit chips for signal processing.While advancements in MEMS and CMOS technology have significantly improved these components,their interconnection still relies on conventional printed circuit boards and sophisticated adapters.This conventional approach adds considerable weight and volume to the package,especially for high channel count systems.To address this issue,we developed a through-polymer via(TPV)method inspired by the through-silicon via(TSV)technique in advanced three-dimensional packaging.This innovation enables the vertical integration of flexible probes,amplifier chips,and PCBs,realizing a flexible,lightweight,and integrated device(FLID).The total weight of the FLIDis only 25%that of its conventional counterparts relying on adapters,which significantly increased the activity levels of animals wearing the FLIDs to nearly match the levels of control animals without implants.Furthermore,by incorporating a platinum-iridium alloy as the top layer material for electrical contact,the FLID realizes exceptional electrical performance,enabling in vivo measurements of both local field potentials and individual neuron action potentials.These findings showcase the potential of FLIDs in scaling up implantable neural recording systems and mark a significant advancement in the field of neurotechnology.