The core G protein signaling module,which consists of Gαand extra-large Gα(XLG)subunits coupled with the Gβγdimer,is a master regulator of various stress responses.In this study,we compared the basal and salt stre...The core G protein signaling module,which consists of Gαand extra-large Gα(XLG)subunits coupled with the Gβγdimer,is a master regulator of various stress responses.In this study,we compared the basal and salt stress-induced transcriptomic,metabolomic and phenotypic profiles in Gα,Gβ,and XLG-null mutants of two plant species,Arabidopsis thaliana and Marchantia polymorpha,and showed that G protein mediates the shift of transcriptional and metabolic homeostasis to stress readiness status.We demonstrated that such stress readiness serves as an intrinsic protection mechanism against further stressors through enhancing the phenylpropanoid pathway and abscisic acid responses.Furthermore,WRKY transcription factors were identified as key intermediates of G protein-mediated homeostatic shifts.Statistical and mathematical model comparisons between A.thaliana and M.polymorpha revealed evolutionary conservation of transcriptional and metabolic networks over land plant evolution,whereas divergence has occurred in the function of plant-specific atypical XLG subunit.Taken together,our results indicate that the shifts in transcriptional and metabolic homeostasis at least partially act as the mechanisms of G protein-coupled stress responses that are conserved between two distantly related plants.展开更多
Saline-alkaline soils are found worldwide, affecting over 1 billion hectares of arable lands especially in arid and semiarid regions. Alkaline soils are characterized by the high pH and low nutrient availability as we...Saline-alkaline soils are found worldwide, affecting over 1 billion hectares of arable lands especially in arid and semiarid regions. Alkaline soils are characterized by the high pH and low nutrient availability as well as high concentrations of bicarbonate and carbonate, being highly detrimental to crop production. According to the UN Food and Agriculture Organization ( FAO and ITPS, 2015 ), approximately 60% of saline soils are concurrently affected by alkalization, posing a serious threat to food security. In recent decades, the breeding objectives for staple crops were mainly focused on yield, quality, and resistance to diseases, leaving alkaline tolerance largely overlooked. Now more than ever, we need to understand the mechanisms of plant alkaline tolerance to develop new crop varieties better adapted to alkaline soil conditions.展开更多
基金This study was supported by the Agency for Science,Technology and Research(A*STAR)Singapore under the Industry Alignment Fund Pre-positioning Program,the High Performance Precision Agriculture(HiPPA)system(A19E4a0101)the Singapore-MIT Aliance for Research and Technology,and Disruptive&Sustainable Technologies for Agricul-tural Precision(DISTAP)(to D.U.)a discovery grant from the Natural Sciences and Engineering Research Council of Canada(ARGPIN-2020-07097)(to K-LL)。
文摘The core G protein signaling module,which consists of Gαand extra-large Gα(XLG)subunits coupled with the Gβγdimer,is a master regulator of various stress responses.In this study,we compared the basal and salt stress-induced transcriptomic,metabolomic and phenotypic profiles in Gα,Gβ,and XLG-null mutants of two plant species,Arabidopsis thaliana and Marchantia polymorpha,and showed that G protein mediates the shift of transcriptional and metabolic homeostasis to stress readiness status.We demonstrated that such stress readiness serves as an intrinsic protection mechanism against further stressors through enhancing the phenylpropanoid pathway and abscisic acid responses.Furthermore,WRKY transcription factors were identified as key intermediates of G protein-mediated homeostatic shifts.Statistical and mathematical model comparisons between A.thaliana and M.polymorpha revealed evolutionary conservation of transcriptional and metabolic networks over land plant evolution,whereas divergence has occurred in the function of plant-specific atypical XLG subunit.Taken together,our results indicate that the shifts in transcriptional and metabolic homeostasis at least partially act as the mechanisms of G protein-coupled stress responses that are conserved between two distantly related plants.
文摘Saline-alkaline soils are found worldwide, affecting over 1 billion hectares of arable lands especially in arid and semiarid regions. Alkaline soils are characterized by the high pH and low nutrient availability as well as high concentrations of bicarbonate and carbonate, being highly detrimental to crop production. According to the UN Food and Agriculture Organization ( FAO and ITPS, 2015 ), approximately 60% of saline soils are concurrently affected by alkalization, posing a serious threat to food security. In recent decades, the breeding objectives for staple crops were mainly focused on yield, quality, and resistance to diseases, leaving alkaline tolerance largely overlooked. Now more than ever, we need to understand the mechanisms of plant alkaline tolerance to develop new crop varieties better adapted to alkaline soil conditions.
