Some studies have indicated that the Wnt/β-catenin signaling pathway is activated following spinal cord injury, and expression levels of specific proteins, including low-density lipoprotein receptor related protein-6...Some studies have indicated that the Wnt/β-catenin signaling pathway is activated following spinal cord injury, and expression levels of specific proteins, including low-density lipoprotein receptor related protein-6 phosphorylation, β-catenin, and glycogen synthase kinase-3β, are significantly altered. We hypothesized that methylprednisolone treatment contributes to functional recovery after spinal cord injury by inhibiting apoptosis and activating the Wnt/β-catenin signaling pathway. In the current study, 30 mg/kg methylprednisolone was injected into rats with spinal cord injury immediately post-injury and at 1 and 2 days post-injury. Basso, Beattie, and Bresnahan scores showed that methylprednisolone treatment significantly promoted locomotor functional recovery between 2 and 6 weeks post-injury. The number of surviving motor neurons increased, whereas the lesion size significantly decreased following methylprednisolone treatment at 7 days post-injury. Additionally, caspase-3, caspase-9, and Bax protein expression levels and the number of apoptotic cells were reduced at 3 and 7 days post-injury, while Bcl-2 levels at 7 days post-injury were higher in methylprednisolone-treated rats compared with saline-treated rats. At 3 and 7 days post-injury, methylprednisolone up-regulated expression and activation of the Wnt/β-catenin signaling pathway, including low-density lipoprotein receptor related protein-6 phosphorylation, β-catenin, and glycogen synthase kinase-3β phosphorylation. These results indicate that methylprednisolone-induced neuroprotection may correlate with activation of the Wnt/β-catenin signaling pathway.展开更多
Spinal cord injury(SCI)is a common traumatic disease of the central nervous system;it causes serious physical and psychological harm to patients and a huge economic burden on the entire society.[1]The tough situation ...Spinal cord injury(SCI)is a common traumatic disease of the central nervous system;it causes serious physical and psychological harm to patients and a huge economic burden on the entire society.[1]The tough situation of SCI treatment requires further exploration of the mechanism and obtaining a new therapeutic strategy.Traditional Chinese medicine(TCM)is based on syndrome differentiation and treatment and overall concepts,and TCM compounds are used as the main method to treat diseases.However,its specific molecular mechanism has not been elucidated.展开更多
基金supported by the National Natural Science Foundation of China,No.81471854
文摘Some studies have indicated that the Wnt/β-catenin signaling pathway is activated following spinal cord injury, and expression levels of specific proteins, including low-density lipoprotein receptor related protein-6 phosphorylation, β-catenin, and glycogen synthase kinase-3β, are significantly altered. We hypothesized that methylprednisolone treatment contributes to functional recovery after spinal cord injury by inhibiting apoptosis and activating the Wnt/β-catenin signaling pathway. In the current study, 30 mg/kg methylprednisolone was injected into rats with spinal cord injury immediately post-injury and at 1 and 2 days post-injury. Basso, Beattie, and Bresnahan scores showed that methylprednisolone treatment significantly promoted locomotor functional recovery between 2 and 6 weeks post-injury. The number of surviving motor neurons increased, whereas the lesion size significantly decreased following methylprednisolone treatment at 7 days post-injury. Additionally, caspase-3, caspase-9, and Bax protein expression levels and the number of apoptotic cells were reduced at 3 and 7 days post-injury, while Bcl-2 levels at 7 days post-injury were higher in methylprednisolone-treated rats compared with saline-treated rats. At 3 and 7 days post-injury, methylprednisolone up-regulated expression and activation of the Wnt/β-catenin signaling pathway, including low-density lipoprotein receptor related protein-6 phosphorylation, β-catenin, and glycogen synthase kinase-3β phosphorylation. These results indicate that methylprednisolone-induced neuroprotection may correlate with activation of the Wnt/β-catenin signaling pathway.
基金the National Natural Science Foundation of China(No.81801906)the Natural Science Foundation of Shandong Province(No.ZR2018PH024)the Jining Science and Technology Key R&D Plan(No.2017SMNS010).
文摘Spinal cord injury(SCI)is a common traumatic disease of the central nervous system;it causes serious physical and psychological harm to patients and a huge economic burden on the entire society.[1]The tough situation of SCI treatment requires further exploration of the mechanism and obtaining a new therapeutic strategy.Traditional Chinese medicine(TCM)is based on syndrome differentiation and treatment and overall concepts,and TCM compounds are used as the main method to treat diseases.However,its specific molecular mechanism has not been elucidated.
