Protective Effects of Six PPTs on Hypoxia/Reoxygenation Induced Cardiomyocyte Injury by Different Treatments

[Objectives]To detect the protective effects of six protopanaxatriols(PPTs)on hypoxia/reoxygenation(H/R)induced cardiomyocyte injury by different treatments.[Methods]The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay was used for detecting the protective effects of six PPTs including ginsenoside Rg1,Re,Rf,Rg2,(R)Rh1 and(S)Rh1 on cell viability reduced by H/R in different treatments.And the adenosine triphosphate(ATP)content and mitochondrial membrane potential(MMP)were used for detecting the mitochondrial function change during PPTs treatment.[Results]Among six PPTs,ginsenoside Rg1,Re,Rf,Rg2 and(R)Rh1 at the concentration of 12.5μM significantly increased the cell survival when treated before and during H/R.These five PPTs also significantly increased the ATP content and MMP reduced by H/R in the same manner.In comparison,only Rg1 significantly increased the cell viability compared with H/R group by pretreating and treating the cells during hypoxia process.[Conclusions]Different treatments affect the protective effects of PPTs.When treated before and during H/R,ginsenoside Rg1,Re,Rf,Rg2 and(R)Rh1 protect the cardiomyocyte against H/R injury mitochondrial function,and only ginsenoside Rg1 has protective effects when treated before and during hypoxia process.