Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of C.pilosula;nonetheless,until now,no validated ...Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of C.pilosula;nonetheless,until now,no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction(RT–qPCR)data.Methods:To screen stable reference genes of S.ureilytica DW2,the expression of its eight candidate reference genes(16S rRNA,ftsZ,ftsA,mreB,recA,slyD,thiC,and zipA)under different treatment conditions(pH,temperature,culture time,and salt content)was assayed by RT–qPCR.The expression stability of these genes was analyzed using different algorithms(geNorm,NormFinder,and BestKeeper).To verify the reliability of the data,the expression of the glucose dehydrogenase(gdh)gene under different soluble phosphate levels was quantified using the most stably expressed reference gene.Results:The results showed that the zipA and 16S rRNA genes were the most stable reference genes,and the least stable genes were thiC and recA.The expression of gdh was consistent with the phosphate solubilization ability on plates containing the National Botanical Research Institute phosphate growth medium.Conclusion:Therefore,this study provides a stable and reliable reference gene of Serratia for the accurate quantification of functional gene expression in future studies.展开更多
Multichannel synthetic aperture radar (SAR) in azimuth can resolve the contradiction between high resolution and wide swath faced with traditional SAR imaging. However, channel errors will degrade the performance of i...Multichannel synthetic aperture radar (SAR) in azimuth can resolve the contradiction between high resolution and wide swath faced with traditional SAR imaging. However, channel errors will degrade the performance of imaging. This paper compares the performances of four channel error estimation algorithms under different clutter distributions and SNR conditions. Further, explanations are given for performance differences of the four algorithms, which provide evidence for method selection in engineering applications.展开更多
Strain and stress were simulated using finite element method(FEM)for threeⅢ-V-on-Insulator(Ⅲ-VOI)structures,i.e.,InP/SiO2/Si,InP/Al2O3/SiO2/Si,and GaAs/Al2O3/SiO2/Si,fabricated by ion-slicing as the substrates for o...Strain and stress were simulated using finite element method(FEM)for threeⅢ-V-on-Insulator(Ⅲ-VOI)structures,i.e.,InP/SiO2/Si,InP/Al2O3/SiO2/Si,and GaAs/Al2O3/SiO2/Si,fabricated by ion-slicing as the substrates for optoelectronic devices on Si.The thermal strain/stress imposes no risk for optoelectronic structures grown on InPOI at a normal growth temperature using molecular beam epitaxy.Structures grown on GaAsOI are more dangerous than those on InPOI due to a limited critical thickness.The intermedia Al2O3 layer was intended to increase the adherence while it brings in the largest risk.The simulated results reveal thermal stress on Al2O3 over 1 GPa,which is much higher than its critical stress for interfacial fracture.InPOI without an Al2O3 layer is more suitable as the substrate for optoelectronic integration on Si.展开更多
Erythropoiesis is a complex and sophisticated multi-stage process regulated by a variety of factors,including the transcription factor GATA1 and non-coding RNA.GATA1 is regarded as an essential transcriptional regulat...Erythropoiesis is a complex and sophisticated multi-stage process regulated by a variety of factors,including the transcription factor GATA1 and non-coding RNA.GATA1 is regarded as an essential transcriptional regulator promoting transcription of erythroidspecific genes—such as long non-coding RNAs(lncRNA).Here,we comprehensively screened lncRNAs that were potentially regulated by GATA1 in erythroid cells.We identified a novel lncRNA—PCED1B-AS1—and verified its role in promoting erythroid differentiation of K562 erythroid cells.We also predicted a model in which PCED1B-AS1 participates in erythroid differentiation via dynamic chromatin remodeling involving GATA1.The relationship between lncRNA and chromatin in the process of erythroid differentiation remains to be revealed,and in our study we have carried out preliminary explorations.展开更多
基金supported by the General Program of Natural Science Foundation of China(32071770)the Fundamental Research Program of Shanxi Province(Award No.202103021223380)the Fund for Shanxi“1331 Project”Key Subjects Construction(1331KSC).
文摘Background:Serratia ureilytica DW2 is a highly efficient phosphate-solubilizing bacteria isolated from Codonopsis pilosula rhizosphere soil that can promote the growth of C.pilosula;nonetheless,until now,no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction(RT–qPCR)data.Methods:To screen stable reference genes of S.ureilytica DW2,the expression of its eight candidate reference genes(16S rRNA,ftsZ,ftsA,mreB,recA,slyD,thiC,and zipA)under different treatment conditions(pH,temperature,culture time,and salt content)was assayed by RT–qPCR.The expression stability of these genes was analyzed using different algorithms(geNorm,NormFinder,and BestKeeper).To verify the reliability of the data,the expression of the glucose dehydrogenase(gdh)gene under different soluble phosphate levels was quantified using the most stably expressed reference gene.Results:The results showed that the zipA and 16S rRNA genes were the most stable reference genes,and the least stable genes were thiC and recA.The expression of gdh was consistent with the phosphate solubilization ability on plates containing the National Botanical Research Institute phosphate growth medium.Conclusion:Therefore,this study provides a stable and reliable reference gene of Serratia for the accurate quantification of functional gene expression in future studies.
文摘Multichannel synthetic aperture radar (SAR) in azimuth can resolve the contradiction between high resolution and wide swath faced with traditional SAR imaging. However, channel errors will degrade the performance of imaging. This paper compares the performances of four channel error estimation algorithms under different clutter distributions and SNR conditions. Further, explanations are given for performance differences of the four algorithms, which provide evidence for method selection in engineering applications.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFE0131300)the National Natural Science Foundation of China(Grant Nos.U1732268,61874128,11622545,61851406,11705262,and 61804157)+5 种基金the Frontier Science Key Program of the Chinese Academy of Sciences(Grant No.QYZDY-SSW-JSC032)the Chinese-Austrian Cooperative Research and Development Project(Grant No.GJHZ201950)the Science and Technology Innovation Action Plan Program of Shanghai,China(Grant No.17511106202)the Program of Shanghai Academic Research Leader,China(Grant No.19XD1404600)the Sailing Program of Shanghai,China(Grant Nos.19YF1456200 and 19YF1456400)the K C Wong Education Foundation(Grant No.GJTD-2019-11).
文摘Strain and stress were simulated using finite element method(FEM)for threeⅢ-V-on-Insulator(Ⅲ-VOI)structures,i.e.,InP/SiO2/Si,InP/Al2O3/SiO2/Si,and GaAs/Al2O3/SiO2/Si,fabricated by ion-slicing as the substrates for optoelectronic devices on Si.The thermal strain/stress imposes no risk for optoelectronic structures grown on InPOI at a normal growth temperature using molecular beam epitaxy.Structures grown on GaAsOI are more dangerous than those on InPOI due to a limited critical thickness.The intermedia Al2O3 layer was intended to increase the adherence while it brings in the largest risk.The simulated results reveal thermal stress on Al2O3 over 1 GPa,which is much higher than its critical stress for interfacial fracture.InPOI without an Al2O3 layer is more suitable as the substrate for optoelectronic integration on Si.
基金supported by the National Key R&D Program of China(2017YFE0131300)the National Natural Science Foundation of China(62174167,61874128)+4 种基金the Frontier Science Key Program of CAS(QYZDY-SSW-JSC032)the Key Research Project of Zhejiang Laboratory(2021MD0AC01)the Program of Shanghai Academic Research Leader(19XD1404600)K.C.Wong Education Foundation(GJTD-2019-11)NCBiR within the Polish-China(WPC/130/NIR-Si/2018)。
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16010602)the National Natural Science Foundation of China(81670109,81700097,81870097,81700116).
文摘Erythropoiesis is a complex and sophisticated multi-stage process regulated by a variety of factors,including the transcription factor GATA1 and non-coding RNA.GATA1 is regarded as an essential transcriptional regulator promoting transcription of erythroidspecific genes—such as long non-coding RNAs(lncRNA).Here,we comprehensively screened lncRNAs that were potentially regulated by GATA1 in erythroid cells.We identified a novel lncRNA—PCED1B-AS1—and verified its role in promoting erythroid differentiation of K562 erythroid cells.We also predicted a model in which PCED1B-AS1 participates in erythroid differentiation via dynamic chromatin remodeling involving GATA1.The relationship between lncRNA and chromatin in the process of erythroid differentiation remains to be revealed,and in our study we have carried out preliminary explorations.
