Effects of Bisphenol A on Testosterone Levels and Sexual Behaviors of Male Mice

Objective: To evaluate the effects of bisphenol A (BPA) on testosterone levels and sexual behaviors of male mice. Methods: Forty 12-week-old male mice, 22 - 25 g, were randomly allocated into four equal groups (n = 10 per group): the control group and three BPA exposure groups including low concentration group (10 mg/kg), middle concentration group (50 mg/kg) and high concentration group (100 mg/kg). Each mouse received BPA intraperitoneally injected for 21 consecutive days. Sexual behaviors and testosterone levels in serum and interstitial tissue of testis were measured after the last administration of BPA. Furthermore, we also observed the weights of sexual organs of each group, including testis, epididymis and seminal vesicle. Results: The mount latency in the high concentration group, the intromission latency in the middle and the high concentration group were 11.64 ± 2.67 min, 20.28 ± 3.40 min and 20.13 ± 2.06 min respectively. All of them were longer than the control group (all P < 0.05). The mount frequency, intromission frequency and copulatory efficacy in the high concentration group were 0.52 ± 0.15 numbers/min, 0.37 ± 0.12 numbers/min and 0.40 ± 0.03, which were all statistically lower than the control group (all P < 0.05). The average testis weights in the low, middle and high concentration group were 0.198 ± 0.032 g, 0.203 ± 0.037g and 0.183 ± 0.032 g, and the relative testis weight in the high concentration group was 0.637 ± 0.106. All of these data were lower than the control group (P < 0.05, P < 0.05, P < 0.01 and P < 0.05, respectively). The epididymis weight and the relative epididymis weight, the weight of seminal vesicle and the relative weight of seminal vesicle were 0.069 ± 0.010 g, 0.242 ± 0.040, 0.219 ± 0.042 g and 0.760 ± 0.143, and all of them were lower than the control group (all P < 0.05). The serum testosterone levels in the high concentration group, the intratesticular testosterone levels in the middle and the high concentration group were 7.88 ± 1.62 ng/ml, 75.5 ± 7.18 ng/g and 73.00 ± 9.57 ng/g, which were all lower than the control group (all P < 0.05). Conclusions: BPA can decline the testosterone levels in mice and inhibit their sexual behaviors.

建筑室内装饰装修技术管理问题和措施

建筑装修不仅要有实用性,也要满足居民对于建筑的美观性、功能性需求。建筑的装饰装修是建筑工程中的重要内容,关系着建筑的质量水平,建筑室内装饰装修工作十分重要,影响着建筑行业的经济发展。据此,笔者就建筑室内装饰装修质量和技术管理,以及应对措施,进行了研究分析。

Mycorrhizal Networks Interacting with Litter Improves Nutrients and Growth for One Plant through the Vary of N/P Ratio under Karst Soil

Arbuscular mycorrhizae(AM)fungi affect nutrient uptake for host plants,while it is unclear how AM fungi interacting with soil litter affect plant growth and nutrient utilization through mycorrhizal networks in karst soil of deficient nutrients beyond the rhizosphere.An experiment was conducted in a microcosm composed of a planting compartment for Cinnamomum camphora seedlings with or without Glomus mosseae fungus(M+vs.M−)and an adjacent litter compartment containing or not containing additional litter material of Arthraxon hispidus(L+vs.L−),where the compartments are connected either by nylon mesh of 20μm or 0.45μm which either allow available mycorrhizal networks within the litter compartment or prevent mycelium entering into the litter compartment(N+vs.N−).Plant biomass and nutrients were measured.The results showed that the addition of litter changed the symbiotic process in mycorrhizal colonization,spore,and hyphal density,which when in association with the host plant then affected the biomass,and accumulations of N(nitrogen)and P(phosphorus)in the individual plant as well as root,stem,and leaf respectively.AM fungi increased N and P accumulations and N/P ratio in individual plants and plant tissues.A decrease of the N/P ratio of the individual plant was observed when AM fungus interacted significantly with litter through mycorrhizal networks in the litter compartment.The results indicate that the C.camphora seedlings benefited from litter in nutrient utilization of N and P through the vary of N/P ratio when accessing mycorrhizal networks.These findings suggest that mycorrhizal networks interacting with litter improve growth and nutrients of N and P for plants through the vary of N/P ratio in order to alleviate nutrient limitation under karst soil.

Reflections on the High-quality Development of Rural Areas in Guangshan County,Xinyang City

The problems faced by the development of Guangshan County were analyzed from the aspects of rural management talents,industrial structure,e-commerce development,and farmers'ideology,and corresponding solutions were proposed to improve the development speed of rural areas in the county and promote the high-quality development of the county as a whole.

Research on the Development of Rural E-commerce in China

At present,rural e-commerce is characterized by obvious improvement in market prospect,development scale and development momentum,but there are also some problems,such as farmers’low acceptance of e-commerce,lack of promotion and training of rural e-commerce,and lack of rural e-commerce talents.Based on these problems,the government needs to increase financial support for rural e-commerce,improve the distribution logistics system,and strengthen the training of rural e-commerce personnel to promote the sustainable development of rural e-commerce.

Challenges and perspectives of covalent organic frameworks for advanced alkali-metal ion batteries

Covalent organic frameworks(COFs)are a class of porous crystalline polymers that have been widely investigated in various fields,including energy storage,photo/electrocatalysis,drug delivery.The covalent-bond interconnection allows COFs extraordinary chemical and thermal stability,and the porous structure ensures a high ion-diffusion coefficient.These merits compensate for the drawbacks of organic electrodes that are easy to dissolve and have low charge conductivity,and promote the development of novel electrode materials with excellent performance,environmental friendliness,and low price.However,the application of COFs also encountered many problems,such as poor electronic conductivity due to the large band gap.Moreover,in some three-dimensional(3D)COFs and stacked two-dimensional(2D)COFs,the huge crystal structure,aligned ultralong channels,and numerous crystal defects usually impede ion transport,and the large molecular weights of COFs generally decrease the specific capacities.These issues are urgently needed to be solved.Here in this review,we summarize the latest progress,core challenges and coping strategies concerning with the use of COFs in alkali-metal ion batteries,discuss the impact of material structure on energy storage,and propose strategies for the construction of high-performance COF-based electrodes.

LncRNA RP11-307C12.11 promotes the growth of hepatocellular carcinoma by acting as a molecular sponge of miR-138

Background:Abnormal expression of long non-coding RNAs(lncRNAs)has been found in almost all tumors in humans,providing numerous potential diagnostic and prognostic biomarkers,and therapeutic targets.Materials and methods:The Cancer Genome Atlas(TCGA)database was used to screen potential LncRNAs,and 30 paired hepatocellular carcinoma(HCC)tissues were used to investigate RP11-307C12.11 expression levels by qRT-PCR and another 105 HCC tissues by in situ hybridizsation(ISH).RP11-307C12.11 overexpression and knockdown experiments were performed to investigate the effects of RP11-307C12.11 on HCC growth through in vitro and in vivo assays(MTT assay,colony formation assay,EdU assay,and xenograft model).The molecular mechanism underlying these effects was confirmed by MS2-RIP-assay,RIP assay,luciferase assay,and rescue experiments.Results:RP11-307C12.11 expression level was significantly higher in tumor tissues than in the adjacent normal tissues.Elevated RP11-307C12.11 expression level was associated with poor prognosis of HCC patients,and it may be represented as an independent prognostic biomarker in patients with HCC.Functionally,RP11-307C12.11 overexpression promoted HCC growth both in vitro and in vivo;however,its knockdown reversed these effects.Mechanistically,we found that RP11-307C12.11 expressed predominantly in the cytoplasm and sponged microRNA(miR)-138 to regulate its common target CCND1 and PDK1.Conclusions:Thus,we found that RP11-307C12.11 acts as an oncogene in HCC by binding to miR-138,which might provide a novel target for HCC therapy.

Multi-functionalized nanofibers with reactive oxygen species scavenging capability and fibrocartilage inductivity for tendon-bone integration

The presence of excessive reactive oxygen species(ROS)after injuries to the enthesis could lead to cellular oxidative damage,high inflammatory response,chronic inflammation,and limited fibrochondral inductivity,making tissue repair and functional recovery difficult.Here,a multifunctional silk fibroin nanofiber modified with polydopamine and kartogenin was designed and fabricated to not only effectively reduce inflammation by scavenging ROS in the early stage of the enthesis healing but also enhance fibrocartilage formation with fibrochondrogenic induction in the later stages.The in vitro results confirmed the antioxidant capability and the fibrochondral inductivity of the functionalized nanofibers.In vivo studies showed that the multifunctional nanofiber can significantly improve the integration of tendon-bone and accelerate the regeneration of interface tissue,resulting in an excellent biomechanical property.Thus,the incorporation of antioxidant and bio-active molecules into extracellular matrix-like biomaterials in interface tissue engineering provides an integrative approach that facilitates damaged tissue regeneration and functional recovery,thereby improving the clinical outcome of the engineered tissue.

AM真菌和枯落物互作下两种喀斯特植物种间竞争较种内竞争更能促进植物养分利用

枯落物是植物养分获取和土壤养分转化的关键载体。丛枝菌根(Arbuscular mycorrhizae,AM)对植物养分摄取的影响已被广泛认知。然而,在养分亏缺的喀斯特生境中,不同竞争方式的植物如何通过AM真菌和枯落物利用养分尚不清楚。本研究对两种喀斯特适生植物构树(Broussonetia papyrifera)和云贵鹅耳枥(Carpinus pubescens)进行种内竞争和种间竞争种植处理,并通过幼套球囊霉(Glomus etunicatum)接种或不接种处理,以及土壤中添加或不添加两物种叶片混合枯落物处理,测定了植物生物量以及氮、磷、钾浓度等指标,研究植物的生长和养分利用。研究结果表明,AM真菌对两种植物养分摄取影响不同,AM真菌显著提高了种内和种间竞争下构树的养分摄取量,但降低了云贵鹅耳枥的养分摄取量。种间竞争下接种AM真菌,枯落物添加促进了云贵鹅耳枥对氮的摄取,抑制了构树对氮的摄取。接种AM真菌和添加枯落物条件下,种间竞争的构树对氮、磷和钾的摄取量及云贵鹅耳枥对氮的摄取量均高于种内竞争;种间竞争下两物种养分竞争力呈现明显差异,即构树对磷和钾养分竞争力显著提高,对氮则不显著;云贵鹅耳枥仅对钾的养分竞争力显著降低,对氮和磷则无显著影响。这些结果说明,在AM真菌与枯落物相互作用下,两种喀斯特植物种间竞争较种内竞争更能促进植物养分利用。