Cell cycle regulation plays a critical role in plant growth and development.In this study,the role of a tomato cell cycle gene SlCycB1 has been characterized.Expression analysis revealed that SlCycB1 was mostly expres...Cell cycle regulation plays a critical role in plant growth and development.In this study,the role of a tomato cell cycle gene SlCycB1 has been characterized.Expression analysis revealed that SlCycB1 was mostly expressed in stem,root,and leaves,with relative lower expression in flower and fruit.Tomato plants overexpressing SlCycB1 exhibited a reduction in cell number and increased cell size leading to the growth retardation.Furthermore,yeast two-hybrid analysis and bimolecular fluorescence complementation revealed that SlCycB1 interacted with histone H3.2,an essential component of the nucleosome.Histone H3.2 was transcriptionally up-regulated in the SlCycB1 overexpressing tomato lines.Furthermore,the overexpression of histone H3.2 in transgenic plants showed similar phenotypes to SlCycB1 overexpressing lines.Based on these findings,we concluded that SlCycB1 overexpression altered tomato architecture in association with histone H3.2.展开更多
The Dof （DNA binding with One Finger） family encoding single zinc finger proteins has been known as a family of plant-specific transcription factors. These transcription factors are involved in a variety of function...The Dof （DNA binding with One Finger） family encoding single zinc finger proteins has been known as a family of plant-specific transcription factors. These transcription factors are involved in a variety of functions of importance for different biological processes in plants. In the current study, we identified 34 Dof family genes in tomato （Solanum lycopersicum L.）, distributed on 11 chromosomes. A complete overview of SlDof genes in tomato is presented, including the gene structures, chromosome locations, phylogeny, protein motifs and evolution pattern. Phylogenetic analysis of 34 SlDof proteins resulted in four classes constituting six clusters. In addition, a comparative analysis between these genes in tomato, Arabidopsis （Arabidopsis thaliana L.） and rice （Oryza sativa L.） was also performed. The tomato Dof family expansion has been dated to recent duplication events, and segmental duplication is predominant for the SlDof genes. Furthermore, the SlDof genes displayed differential expression either in their transcript abundance or in their expression patterns under normal growth conditions. This is the first step towards genome-wide analyses of the Dof genes in tomato. Our study provides a very useful reference for cloning and functional analysis of the members of this gene family in tomato and other species.展开更多
基金This work was supported by grants from the National Key Research&Development Plan(Grant Nos.2021YFD12002012018YFD1000800)+4 种基金National Natural Science Foundation of China(Grant Nos.3199118231972426)Wuhan Frontier Projects for Applied Foundation(Grant No.2019020701011492)International Cooperation Promotion Plan of Shihezi University(Grant No.GJHZ202104)Key Project of Hubei Hongshan Laboratory(Grant No.2021hszd007).
文摘Cell cycle regulation plays a critical role in plant growth and development.In this study,the role of a tomato cell cycle gene SlCycB1 has been characterized.Expression analysis revealed that SlCycB1 was mostly expressed in stem,root,and leaves,with relative lower expression in flower and fruit.Tomato plants overexpressing SlCycB1 exhibited a reduction in cell number and increased cell size leading to the growth retardation.Furthermore,yeast two-hybrid analysis and bimolecular fluorescence complementation revealed that SlCycB1 interacted with histone H3.2,an essential component of the nucleosome.Histone H3.2 was transcriptionally up-regulated in the SlCycB1 overexpressing tomato lines.Furthermore,the overexpression of histone H3.2 in transgenic plants showed similar phenotypes to SlCycB1 overexpressing lines.Based on these findings,we concluded that SlCycB1 overexpression altered tomato architecture in association with histone H3.2.
基金supported by the State Major Basic Research Development Program (2011CB100600)the National Natural Science Foundation of China (31171974 and 30800755)
文摘The Dof （DNA binding with One Finger） family encoding single zinc finger proteins has been known as a family of plant-specific transcription factors. These transcription factors are involved in a variety of functions of importance for different biological processes in plants. In the current study, we identified 34 Dof family genes in tomato （Solanum lycopersicum L.）, distributed on 11 chromosomes. A complete overview of SlDof genes in tomato is presented, including the gene structures, chromosome locations, phylogeny, protein motifs and evolution pattern. Phylogenetic analysis of 34 SlDof proteins resulted in four classes constituting six clusters. In addition, a comparative analysis between these genes in tomato, Arabidopsis （Arabidopsis thaliana L.） and rice （Oryza sativa L.） was also performed. The tomato Dof family expansion has been dated to recent duplication events, and segmental duplication is predominant for the SlDof genes. Furthermore, the SlDof genes displayed differential expression either in their transcript abundance or in their expression patterns under normal growth conditions. This is the first step towards genome-wide analyses of the Dof genes in tomato. Our study provides a very useful reference for cloning and functional analysis of the members of this gene family in tomato and other species.
