Objective:To investigate granuloma associated mycobacteriosis in Pampus argenteus(P.argenteus)(silver pomfrets)and its seasonal prevalence in marine and coastal aquaculture systems of West Bengal,India.Methods:P.argen...Objective:To investigate granuloma associated mycobacteriosis in Pampus argenteus(P.argenteus)(silver pomfrets)and its seasonal prevalence in marine and coastal aquaculture systems of West Bengal,India.Methods:P.argenteus having granulomatous infection were sampled from coastal areas of East Midnapore District of West Bengal from January 2014 to December 2015,encompassing the various seasons.Tissue samples were collected for confirmation by biochemical studies and their prevalence and degree of infection and histopathology was conducted.Results:A total of 197 fishes having haemorrhage like infections in skin and viscera were confirmed for mycobacteria by biochemical analysis,with prevalence of 22.6%.Post-winter season showed higher prevalence(47.97%)followed by winter(35.25%),pre-winter(27.81%),summer(12.5%)and rainy season(2.57%)respectively.Histopathological assessment showed necrotic granuloma in skin,gills and some internal tissues.Ziehl-Neelsen staining of infected tissue samples showed the deposition of acid fast organisms causing swelling and haemorrhages in the tissues.Severity of infection was evaluated by mean cumulative granuloma score(7.89)score.Majority of the acid fast bacilli were recovered from skin,gills and other organs.Biochemical assessment of the acid fast rods confirmed the presence of Mycobacterium spp.Conclusions:The result suggests that the nontuberculous mycobacterial species are responsible for the granulomatous infection in P.argenteus.This infection seems to be influenced by environmental temperature,with the lower temperature favoring the pathogens.展开更多
文摘Objective:To investigate granuloma associated mycobacteriosis in Pampus argenteus(P.argenteus)(silver pomfrets)and its seasonal prevalence in marine and coastal aquaculture systems of West Bengal,India.Methods:P.argenteus having granulomatous infection were sampled from coastal areas of East Midnapore District of West Bengal from January 2014 to December 2015,encompassing the various seasons.Tissue samples were collected for confirmation by biochemical studies and their prevalence and degree of infection and histopathology was conducted.Results:A total of 197 fishes having haemorrhage like infections in skin and viscera were confirmed for mycobacteria by biochemical analysis,with prevalence of 22.6%.Post-winter season showed higher prevalence(47.97%)followed by winter(35.25%),pre-winter(27.81%),summer(12.5%)and rainy season(2.57%)respectively.Histopathological assessment showed necrotic granuloma in skin,gills and some internal tissues.Ziehl-Neelsen staining of infected tissue samples showed the deposition of acid fast organisms causing swelling and haemorrhages in the tissues.Severity of infection was evaluated by mean cumulative granuloma score(7.89)score.Majority of the acid fast bacilli were recovered from skin,gills and other organs.Biochemical assessment of the acid fast rods confirmed the presence of Mycobacterium spp.Conclusions:The result suggests that the nontuberculous mycobacterial species are responsible for the granulomatous infection in P.argenteus.This infection seems to be influenced by environmental temperature,with the lower temperature favoring the pathogens.
