Osmotic conditions play an important role in the cell properties of human red blood cells(RBCs),which are crucial for the pathological analysis of some blood diseases such as malaria.Over the past decades,numerous eff...Osmotic conditions play an important role in the cell properties of human red blood cells(RBCs),which are crucial for the pathological analysis of some blood diseases such as malaria.Over the past decades,numerous efforts have mainly focused on the study of the RBC biomechanical properties that arise from the unique deformability of erythrocytes.Here,we demonstrate nonlinear optical effects from human RBCs suspended in different osmotic solutions.Specifically,we observe self-trapping and scattering-resistant nonlinear propagation of a laser beam through RBC suspensions under all three osmotic conditions,where the strength of the optical nonlinearity increases with osmotic pressure on the cells.This tunable nonlinearity is attributed to optical forces,particularly the forward-scattering and gradient forces.Interestingly,in aged blood samples(with lysed cells),a notably different nonlinear behavior is observed due to the presence of free hemoglobin.We use a theoretical model with an optical force-mediated nonlocal nonlinearity to explain the experimental observations.Our work on light self-guiding through scattering biosoft-matter may introduce new photonic tools for noninvasive biomedical imaging and medical diagnosis.展开更多
基金the National Key R&D Program of China(2017YFA0303800)the National Natural Science Foundation of China(91750204,11504184,11604058)+3 种基金the NSERC through the Steacie,Strategic,Discovery and Acceleration Grants Schemesthe Canada Research Chair Program(Canada)additional support by the Government of the Russian Federation through the ITMO Fellowship and Professorship Program(grant 074-U 01)the 1000 Talents Sichuan Program in China.
文摘Osmotic conditions play an important role in the cell properties of human red blood cells(RBCs),which are crucial for the pathological analysis of some blood diseases such as malaria.Over the past decades,numerous efforts have mainly focused on the study of the RBC biomechanical properties that arise from the unique deformability of erythrocytes.Here,we demonstrate nonlinear optical effects from human RBCs suspended in different osmotic solutions.Specifically,we observe self-trapping and scattering-resistant nonlinear propagation of a laser beam through RBC suspensions under all three osmotic conditions,where the strength of the optical nonlinearity increases with osmotic pressure on the cells.This tunable nonlinearity is attributed to optical forces,particularly the forward-scattering and gradient forces.Interestingly,in aged blood samples(with lysed cells),a notably different nonlinear behavior is observed due to the presence of free hemoglobin.We use a theoretical model with an optical force-mediated nonlocal nonlinearity to explain the experimental observations.Our work on light self-guiding through scattering biosoft-matter may introduce new photonic tools for noninvasive biomedical imaging and medical diagnosis.
