期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Aged Garlic Extract Reduces ROS Production and Cell Death Induced by 6-Hydroxydopamine through Activation of the Nrf2-ARE Pathway in SH-SY5Y Cells
1
作者 Kohfuku Kohda Hitomi Goda +2 位作者 Kei Itoh Keijiro Samejima tomoko fukuuchi 《Pharmacology & Pharmacy》 2013年第1期31-40,共10页
Many degenerative or pathological processes, such as aging, cancer and coronary heart disease, are related to reactive oxygen species (ROS) and radical-mediated reactions. We examined the effectiveness of aged garlic ... Many degenerative or pathological processes, such as aging, cancer and coronary heart disease, are related to reactive oxygen species (ROS) and radical-mediated reactions. We examined the effectiveness of aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, for protection of cells from ROS produced by 6-hydroxy-dopamine (6-OHDA) using human neuroblastoma SH-SY5Y cells. Concomitant treatment of cells with AGE (2 and 4 mg/ml) showed the dose-dependent protective effect on the cell death induced by 6-OHDA. In addition, the AGE treatment significantly suppressed the increase of ROS generation by 6-OHDA. Furthermore, the protective effect of AGE was accompanied by activation of the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway and the increase of mRNAs of heme oxygenase-1 and NAD(P)H quinone oxidoreductase 1. These two enzymes are important in the cellular antioxidant system. These results indicated that AGE protected cells from ROS damage by not only capturing ROS directly but also activating the cellular antioxidant system by stimulating antioxidant gene expression via the Nrf2-ARE pathway. The present study suggested that AGE may be useful for prevention and treatment of cell damage caused by ROS. 展开更多
关键词 Aged GARLIC Extract (AGE) 6-OHDA ROS Nrf2-ARE PATHWAY SH-SY5Y Cells
下载PDF
Enhancing Effect of Aged Garlic Extract on Induction of Morphological Differentiation with Neurite Outgrowth in NGF-treated PC12 Cells
2
作者 Kohfuku Kohda Kei Itoh +6 位作者 Hitomi Goda Keijiro Samejima tomoko fukuuchi Naoki Morihara Kazuhiko Imamura Yukihiro Kodera Takami Oka 《Pharmacology & Pharmacy》 2012年第1期37-43,共7页
Background: We have been searching effective compounds that can stimulate the growth and differentiation of nerve cells. We found previously that fulleren derivatives enhanced induction of morphological differentiatio... Background: We have been searching effective compounds that can stimulate the growth and differentiation of nerve cells. We found previously that fulleren derivatives enhanced induction of morphological differentiation with neurite outgrowth in nerve growth factor (NGF)-treated PC12 cells. In the course of our further search for other effective compounds, we found the aged garlic extract (AGE) has the activity similar to fulleren. Methods: PC12 cells were used to examine the effectiveness of test compound. Results: AGE enhanced the stimulating effect of NGF to induce morphological differentiation with neurite outgrowth in PC12 cells. In order to examine the active constituents of AGE, it was fractionated into several components. The activity was mainly localized in the F1 fraction that contains low molecular weight polar compounds. S-Allymercaptocysteine (SAMC) is one of the sulfur components of AGE present in F1 fraction and found to exhibit the enhancing effect similar to AGE. Conclusion: AGE had the ability to induce morphological differentiation with neurite outgrowth in NGF-treated PC12 cell and SAMC was one of its active constituents. 展开更多
关键词 Aged GARLIC EXTRACT PC12 Cells NGF Differentiation NEURITE OUTGROWTH
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部