A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length...A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.展开更多
为阐明cilp基因在斑马鱼椎骨和肌间骨发育中的作用,利用CRISPR/Cas9建立斑马鱼cilp基因敲除纯合突变系(cilp^(-/-)),对其进行骨骼表型的观察,并进一步采用qRT-PCR分析14个骨骼发育相关基因在突变体胚胎发育阶段和成鱼骨骼中的表达水平...为阐明cilp基因在斑马鱼椎骨和肌间骨发育中的作用,利用CRISPR/Cas9建立斑马鱼cilp基因敲除纯合突变系(cilp^(-/-)),对其进行骨骼表型的观察,并进一步采用qRT-PCR分析14个骨骼发育相关基因在突变体胚胎发育阶段和成鱼骨骼中的表达水平变化。结果显示:与野生型斑马鱼相比,90 dph(Days post hatching)cilp^(-/-)斑马鱼的肌间骨数量显著减少了10.27%,而肌间骨的长度无明显变化;同时突变体斑马鱼中椎骨发生异常融合及髓棘缺失。qRT-PCR结果显示:与野生型相比,col1a1a、sp7、smad4a和smad5基因在胚胎发育的整个时期都存在显著的差异,在突变体成鱼尾部骨骼组织中bmp2a、bmp2b、smad5、sp7、runx2a、runx2b和bglap基因的表达量均显著低于野生型,表明cilp基因敲除导致了BMPs和SMADs家族基因的表达水平下降,并下调了下游的成骨细胞发育相关基因的表达量,推测cilp功能缺失可能通过抑制BMPs信号通路影响成骨细胞分化和骨形成,从而导致了肌间骨数量的减少和脊椎骨异常融合。展开更多
基金Supported by the National Science and Technology Support Plan Project(2012BDA25B10)the Science and Technology Project of Heilongjiang Province(GZ11B201)+1 种基金the Fundamental Research Fund for Central Public Welfare Research Institutes(201109)the Open Project of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture(2012NYBSJ-03)
文摘A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.
文摘为阐明cilp基因在斑马鱼椎骨和肌间骨发育中的作用,利用CRISPR/Cas9建立斑马鱼cilp基因敲除纯合突变系(cilp^(-/-)),对其进行骨骼表型的观察,并进一步采用qRT-PCR分析14个骨骼发育相关基因在突变体胚胎发育阶段和成鱼骨骼中的表达水平变化。结果显示:与野生型斑马鱼相比,90 dph(Days post hatching)cilp^(-/-)斑马鱼的肌间骨数量显著减少了10.27%,而肌间骨的长度无明显变化;同时突变体斑马鱼中椎骨发生异常融合及髓棘缺失。qRT-PCR结果显示:与野生型相比,col1a1a、sp7、smad4a和smad5基因在胚胎发育的整个时期都存在显著的差异,在突变体成鱼尾部骨骼组织中bmp2a、bmp2b、smad5、sp7、runx2a、runx2b和bglap基因的表达量均显著低于野生型,表明cilp基因敲除导致了BMPs和SMADs家族基因的表达水平下降,并下调了下游的成骨细胞发育相关基因的表达量,推测cilp功能缺失可能通过抑制BMPs信号通路影响成骨细胞分化和骨形成,从而导致了肌间骨数量的减少和脊椎骨异常融合。
