The in vitro metabolism o. butylphthalide (HBP) by rat liver microsomes was studied in this paper. Capillary GC/MS and BSTFA/1%TMCS derivatization were used for the separation and structure identification of the six m...The in vitro metabolism o. butylphthalide (HBP) by rat liver microsomes was studied in this paper. Capillary GC/MS and BSTFA/1%TMCS derivatization were used for the separation and structure identification of the six metabolites. 3-Hydroxylation and r-hydroxylation have been proved to be the main metabolism pathways of HBP by rat liver microsomes.展开更多
A sensitive and selective method has been developed for the detection of free ethacrynic acid in human urine. Using 4-(bromomethyl)-7-methoxycoumarin, ethacrynic acid was transformed into a fluorescent derivative, and...A sensitive and selective method has been developed for the detection of free ethacrynic acid in human urine. Using 4-(bromomethyl)-7-methoxycoumarin, ethacrynic acid was transformed into a fluorescent derivative, and was analysed by HPLC-fluorescence detector. The detection limit is 0.1ug/ml urine. The method is suitable for screening ethacrynic acid in doping control and studying its metabolism.展开更多
Three new metabolites(N-acetyl-pseudoephedrine,N-acetyl- norpseudoephedrine and N,O-diacetylnorpseudoephedrine)of ephedrines were d(?)ected and confirmed by GC/MS method and chemical derivatiza- tion.The metabolic pat...Three new metabolites(N-acetyl-pseudoephedrine,N-acetyl- norpseudoephedrine and N,O-diacetylnorpseudoephedrine)of ephedrines were d(?)ected and confirmed by GC/MS method and chemical derivatiza- tion.The metabolic pathway of acetylation of hydroxyl group is reported for the first time.展开更多
文摘The in vitro metabolism o. butylphthalide (HBP) by rat liver microsomes was studied in this paper. Capillary GC/MS and BSTFA/1%TMCS derivatization were used for the separation and structure identification of the six metabolites. 3-Hydroxylation and r-hydroxylation have been proved to be the main metabolism pathways of HBP by rat liver microsomes.
文摘A sensitive and selective method has been developed for the detection of free ethacrynic acid in human urine. Using 4-(bromomethyl)-7-methoxycoumarin, ethacrynic acid was transformed into a fluorescent derivative, and was analysed by HPLC-fluorescence detector. The detection limit is 0.1ug/ml urine. The method is suitable for screening ethacrynic acid in doping control and studying its metabolism.
文摘Three new metabolites(N-acetyl-pseudoephedrine,N-acetyl- norpseudoephedrine and N,O-diacetylnorpseudoephedrine)of ephedrines were d(?)ected and confirmed by GC/MS method and chemical derivatiza- tion.The metabolic pathway of acetylation of hydroxyl group is reported for the first time.
