The protein assay with bromocresol green (BCG) method is commonly ed because of itsconvenience, but it is not well understood on a molecular level. In this paper, the study on thereaction between BCG and bovine serum ...The protein assay with bromocresol green (BCG) method is commonly ed because of itsconvenience, but it is not well understood on a molecular level. In this paper, the study on thereaction between BCG and bovine serum albumin (BSA) is reported The binding numbers and thebinding conStants are given at different pH by using three methods with relevant comparison. It wasProved that there were three sorts of binding siteS of BSA to react with BCG and BCG transformedinto its boo form in the presence of BSA.展开更多
The binding of bromo-pyrogallol red (BPR) to proteins is accompanied by the enhancement of Rayleigh light scattering at 332nm. and based on this a new quantitative protein assay method is put forward.
文摘The protein assay with bromocresol green (BCG) method is commonly ed because of itsconvenience, but it is not well understood on a molecular level. In this paper, the study on thereaction between BCG and bovine serum albumin (BSA) is reported The binding numbers and thebinding conStants are given at different pH by using three methods with relevant comparison. It wasProved that there were three sorts of binding siteS of BSA to react with BCG and BCG transformedinto its boo form in the presence of BSA.
文摘The binding of bromo-pyrogallol red (BPR) to proteins is accompanied by the enhancement of Rayleigh light scattering at 332nm. and based on this a new quantitative protein assay method is put forward.
