Does nest sanitation elicit egg rejection in an open-cup nesting cuckoo host rejecter?

Background: Nest sanitation behavior is one of the most important means to ensure high reproductive efficiency. In avian brood parasitism, nest sanitation behavior may be a pre-adaptation of host birds that allows them to identify the parasitic eggs, so that egg discrimination behavior may have evolved from nest sanitation behavior. However, whether nest sanitation behavior could improve egg rejection in cuckoo hosts was inconclusive.Methods: In this study, we investigated the relationship between nest sanitation and egg discrimination behavior in a potential cuckoo host, the Brown-breasted Bulbul(Pycnonotus xanthorrhous) with two experimental groups. In the first group, we added a blue, non-mimetic egg to the nest of the host, while in the second group we added a blue, non-mimetic egg and a peanut half-shell.Results: The results showed that in the first group, the probability of rejecting the non-mimetic eggs was 53.8%(n = 26 nests). In comparison, all of the Brown-breasted Bulbuls in the second group were able to rapidly remove the peanut shells from the nest, but only 52.6%(n = 19 nests) rejected the non-mimetic eggs. The rejection rates of the non-mimetic eggs in both experimental groups were not significantly different.Conclusions: Our study indicated that nest sanitation behavior of Brown-breasted Bulbuls did not influence their egg recognition and that egg discrimination ability of Brown-breasted Bulbuls was not directly related to nest sanitation behavior.

Russet Sparrows spot alien chicks from their nests

Background： In coevolutionary interactions between brood parasites and their hosts, host parents are under strong selection to evolve defenses against parasitism. Egg rejection is an efficient and common defense against parasitism, although some apparently suitable hosts do not reject cuckoo eggs.Methods： Sparrows Ploceidae are widespread throughout the Old World, and they have a suitable diet for rearing cuckoos, but still they are rarely exploited by brood parasites. To solve such puzzle, we conducted artificial parasitism and cross-fostering experiments in Russet Sparrow （Posset cinnomomeus）.Results： The present study showed that Russet Sparrows have no egg recognition ability, but recognize their own nestlings and eject alien chicks or starve them to death. They may use visual cues in chick recognition, although they accept sister species Tree Sparrow （Posset montonus）.Conclusions： By rejecting nestlings of foreign species, Russet Sparrows have succeeded to escape from the brood parasitism by cuckoos and other parasites. Our studies shed light on the puzzle why some species are not utilized by cuckoo parasites as hosts,

Last but not the least: effects of laying sequence on egg color variation and embryonic development of Russet Sparrow(Passer cinnamomeus)

Background: Laying sequence has important effects on eggshell color and embryonic development in birds. Some birds can allocate resources unevenly among the eggs within a clutch, prioritizing those at the beginning of the laying sequence, in order to maximize reproductive success. The changes in egg color according to laying sequence may be an adaptation to pressure from predators or brood parasites.Methods: In this study, effects of laying sequence on egg color and embryonic heart rate in Russet Sparrows(Passer cinnamomeus) were investigated using artificial nest boxes. The eggs were divided into three groups: first to be laid, intermediate in the laying sequence, and last to be laid. We maintained the eggs in an incubator and measured embryonic heart rates.Results: Avian visual modeling showed that the background color brightness of the last eggs laid was significantly higher(whiter) than those of the other eggs. All eggs were about the same size and hatched around 13 days, indicating that laying sequence significantly affected embryonic development speed; the last eggs to be laid developed significantly faster than did the first in the clutch.Conclusions: Our study quantified the effect of laying sequence on egg color variation and proved that laying sequence has an important effect on embryonic heart rate in Russet Sparrows.

Coevolution between Himalayan cuckoos and 2 sympatric Pycnonotidae hosts

Selection due to cuckoo parasitism is responsible for the evolution of anti-parasitism defenses in hosts.Different host species breeding sympatrically with a single parasitic cuckoo may evolve different strategies to reduce the risk of counter cuckoo parasitism,resulting in different interactions between cuckoos and hosts in areas of sympatry.Here,we studied the coevolutionary interactions between Himalayan cuckoos Cuculus saturatus and 2 sympatric and closely related potential hosts belonging to the family Pycnonotidae,the brown-breasted bulbul Pycnonotus xanthorrhous and the collared finchbill Spizixos semitorques.We investigated parasitism rates and nest-site selection(nest height,nest cover,human disturbance,perch height,forest distance,and degree of concealment)related to parasitism risk,nest defense against a cuckoo dummy,and egg rejection against cuckoo model eggs.Bulbuls used specific nest sites that were further away from forests than those of finchbills,and they behaved more aggressively toward cuckoos than finchbills.In contrast,bulbuls possessed moderate egg rejection ability,whereas the finchbill rejected 100%of cuckoo model eggs.We suggest that selection of a nest site away from forests by the bulbul explains the absence of parasitism by Himalayan cuckoos.We suggest that these interspecific differences in nest-site selection and nest defense indicate alternative responses to selection due to cuckoos.